Reviewed, UniProtKB/Swiss-Prot Q2KW44 (PYRD_BORA1)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BAV2753

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024154
Sites
	Active site	183	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KW44-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 2B79F6391D1BAF15	FASTA	349	37,027
	10 20 30 40 50 60 MSILFNAYPL ARPALFAMDA ETAHEVTLAS LQRAYDCGTT RRWLHDQPQL PTTLMGMTLR 70 80 90 100 110 120 NPVGLAAGLD KNGAFIDALG NLGFGFVEVG TVTPRAQSGN PKPRMFRLPK ANALINRLGF 130 140 150 160 170 180 NNQGLDAFLA NVTRSRFRSQ GGILGLNIGK NADTPIERAA DDYLIGLAGV YPHADYVTVN 190 200 210 220 230 240 ISSPNTKNLR ALQGGDELSQ LLAALRDKRA ELAQQHARQV PLVVKIAPDL SQEQIDIIAD 250 260 270 280 290 300 TLLSNGVDGV IATNTTLSRE AVQGMPHAAE TGGLSGAPVH ELSLAVIERL RQRVGSALAI 310 320 330 340 IGVGGILSGQ QAREKIAAGA DAVQLYTGLI YRGPALVGEC VGTLKNTAR

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ50364.1.
RefSeq	YP_787251.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6266676.
GenomeReviews	Gene locus BAV2753 in contig AM167904_GR.
KEGG	bav:BAV2753.
NMPDR	fig|521.1.peg.356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KW44.
OMA	Q2KW44. AALNRMG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BORA1

Accession

Primary (citable) accession number: Q2KW44

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)