Reviewed, UniProtKB/Swiss-Prot Q2KVF5 (MURD_BORA1)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BAV2881

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257168
Regions
	Nucleotide binding	138 – 144	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2KVF5-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 8BB3FE665391C7A1	FASTA	508	53,709
	10 20 30 40 50 60 MNTELTSRAD APLVLILGLG ETGVAAARWC AREGARLRVA DTRMQPGGLE GLRAVLQEAQ 70 80 90 100 110 120 VEYHLGCGSH FDPALLDGVT QLVLSPGLAP GQEPAASLLA AAVSRGIEVL GEVELFARAL 130 140 150 160 170 180 ASLAEARAYH PRLLAVTGTN GKTTVTALTR QLIEACGLSA RAAGNISPAA LASLMEALDQ 190 200 210 220 230 240 DALPDVWVLE LSSFQLETTH SLQADAAVVL NVTQDHLDWH GDMQAYAQAK ARLLKMARVA 250 260 270 280 290 300 IVNRDDPLTL AMVADVNGLN VRSFGRDLPQ RVGDMGLELG QGVAWLVACE PSDFDEPVVR 310 320 330 340 350 360 RKKDAPPPQR GEGRMSRLMP VDALRIRGVH NALNALAALQ LARVLELGWG PMLRALRDYA 370 380 390 400 410 420 GEPHRAAFVR SIGGVDYIND SKGTNVGATV AALEGLGQTV VLIAGGQGKG QDFSPLRSAV 430 440 450 460 470 480 SRHARAVVLI GADGPAIGQV LESTGVALVV AADMREAVRR AAEIAQAGEA VLLSPACASL 490 500 DMYRNYPHRG QVFVEEVEEL ALDRGEVL

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ50491.1.
RefSeq	YP_787377.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6267041.
GenomeReviews	Gene locus BAV2881 in contig AM167904_GR.
KEGG	bav:BAV2881.
NMPDR	fig|521.1.peg.453.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KVF5.
OMA	Q2KVF5. SEDHLDR.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BORA1

Accession

Primary (citable) accession number: Q2KVF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)