Q2KV81 (TPMT_BORA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	BAV1018

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047202
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KV81 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 119D02848A4E0515	FASTA	217	24,508
	10 20 30 40 50 60 MDADFWLDRW REGKTGFHQD RVTPLLPKYW PRLALPTGAR VLVPLAGKSL DMVWLAEQGH 70 80 90 100 110 120 EVLGVELSSL AVQQFFAEQG WAPRRWESAY GQHYAANGVE LICGDIFKLD DAALKDCLGV 130 140 150 160 170 180 YDRAALVALP ETMRRAYANH VYGQMPAGYR GLLLTLDYPQ TQMEGPPFSV DEAEVQALFG 190 200 210 EQAHLWDRRD ILEKEPKFAE RGVSRLDTLV FRLSAAS

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 197N.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM167904 Genomic DNA. Translation: CAJ48627.1.
RefSeq	YP_785541.1. NC_010645.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KV81.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6266584.
GenomeReviews	Gene locus BAV1018 in contig AM167904_GR.
KEGG	bav:BAV1018.
NMPDR	fig|521.1.peg.2766.
PATRIC	21128240. VBIBorAvi43433_1044.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG444929.
OMA	QGDIFTL.
ProtClustDB	PRK13255.
Enzyme and pathway databases
BioCyc	ABAU360910:BAV1018-MONOMER.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR022474. Thiopur_S-MeTfrase_Se/Te_detox. IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
TIGRFAMs	TIGR03840. TMPT_Se_Te. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_BORA1

Accession

Primary (citable) accession number: Q2KV81

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program