Q2KTI4 (KATG_BORA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	BAV3417

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.
Sequence caution	The sequence CAJ51027.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Catalase-peroxidase HAMAP MF_01961		PRO_0000354726
Sites
	Active site	92	1	Proton acceptor By similarity
	Metal binding	277	1	Iron (heme axial ligand) By similarity
	Site	88	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	91 ↔ 236		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-262) By similarity
	Cross-link	236 ↔ 262		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KTI4 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 13D42B441D82CDB5	FASTA	748	82,411
	10 20 30 40 50 60 MSNEAKCPFN HTAGSGTTNQ DWWPKRLRLE LLSQHSAKTN PLDPDFDYAK AFNSLDLAAV 70 80 90 100 110 120 KQDLAKLMTD SQDWWPADFG HYGPLFIRMA WHSAGTYRVG DGRGGAGRGQ QRFAPLNSWP 130 140 150 160 170 180 DNVSLDKARR LLWPIKKKYG NKISWADLFI LTGNVALETM GFKTFGFGGG REDTWEPDQD 190 200 210 220 230 240 VYWGDEKTWL GGDLRYGKNE AQPVADKAGH GKEHGRTDGG RNLENPLAAV QMGLIYVNPE 250 260 270 280 290 300 GPDGNPDPQA SAHDIRETFA RMAMNDEETV ALIAGGHTFG KTHGAGPADN VGPEPEAAEL 310 320 330 340 350 360 ENQGLGWKNS FGTGKGSDTI TSGLEVTWTS TPTKWSNNFF WNLFGYEWEL TKSPAGAHQW 370 380 390 400 410 420 IPKHGAGAGS VPDAHDPSKR HVPSMLTSDI ALRVDPAYEK IARRFFENPD EFADAFARAW 430 440 450 460 470 480 FKLTHRDMGP RVRYLGPEVP AEDLIWQDPI PKVNHPLVDD QDVAALKQKV LASGLSVSEL 490 500 510 520 530 540 VSTAWASAST FRGSDKRGGA NGARIRLAPQ KDWAVNQPEQ LAKVLKVLEG IQADFNGKQS 550 560 570 580 590 600 GGKKVSLADL IVLAGCAAIE QAAGQAGHKV TVPFTAGRMD ASQEQTDVAS FAPLEPVHDG 610 620 630 640 650 660 FRNFLKSRYD VPAEHLLIDR AQLLTLTAPE MTVLIGGLRV LDVHTDKEKH GVFTDRPGVL 670 680 690 700 710 720 TNDFFRNLID MGTEWKPSSP ARESFTGHDR KTGKQKWTAS RVDLVFGSNS QLRALSEVYA 730 740 SDDAQDKFVR DFIAAWTKVM NLDRFDLK

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 197N.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM167904 Genomic DNA. Translation: CAJ51027.1. Different initiation.
RefSeq	YP_787911.1. NC_010645.1.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KTI4.
Protein family/group databases
PeroxiBase	3418. BavCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6267465.
GenomeReviews	Gene locus BAV3417 in contig AM167904_GR.
KEGG	bav:BAV3417.
NMPDR	fig|521.1.peg.1866.
PATRIC	21133165. VBIBorAvi43433_3457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	ABAU360910:BAV3417-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_BORA1

Accession

Primary (citable) accession number: Q2KTI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program