Q2KNB9 (HXK2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hexokinase-2
EC=2.7.1.1

Alternative name(s):
Hexokinase-3

Gene names

Name:	HXK2
Synonyms:	HXK3
Ordered Locus Names:	Os05g0532600, LOC_Os05g45590
ORF Names:	OSJNBa0053E05.8

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. Ref.1
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Tissue specificity	Expressed in roots, leaves, flowers, immature seeds, endosperm and seed coat. Ref.1
Developmental stage	Expressed during flower development until 15 days after flowering. Ref.1
Induction	By glucose or fructose treatment in leaves. Ref.1
Sequence similarities	Belongs to the hexokinase family.
Sequence caution	The sequence AAT47078.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glucose 6-phosphate metabolic process Inferred from electronic annotation. Source: GOC glucose mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene glycolysis Inferred from electronic annotation. Source: UniProtKB-KW hexokinase-dependent signaling Inferred from electronic annotation. Source: EnsemblPlants/Gramene programmed cell death Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	mitochondrion Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleus Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene plastid Inferred from electronic annotation. Source: EnsemblPlants/Gramene vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene glucokinase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene zinc ion binding Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Hexokinase-2		PRO_0000247565
Regions
	Nucleotide binding	98 – 103	6	ATP Potential
	Region	168 – 194	27	Glucose-binding Potential
	Compositional bias	4 – 14	11	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	Q2KNB9 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: CBD4744352238E0D	FASTA	494	53,626
	10 20 30 40 50 60 MRKAAAAAVA AAAAVGVALL VRRQLREAKR WGRADAVLRE LEERCAAPPG RLRQVADAMA 70 80 90 100 110 120 VEMHAGLASE GGSKLKMIIS YVDALPSGEE KGVFYALDLG GTNFRVLRVQ LGGKEGRVIK 130 140 150 160 170 180 QEHDEISIPP HLMTGGSNEL FDFIASSLAK FVASEGEDFH LAEGRQRELG FTFSFPVKQT 190 200 210 220 230 240 SIASGTLINW TKGFSIDETV GEDVVTELTK ALERQGLDMK VTALINDTIG TLAGGRYDDN 250 260 270 280 290 300 DVIAAVILGT GTNAAYVERA NAIPKWHDLL PKSGDMVINM EWGNFRSSHL PLTEFDQALD 310 320 330 340 350 360 AESLNPGEQV YEKLISGMYL GEIVRRVLLK MAEEASLFGD EVPPKLKIPF IIRTPYMSMM 370 380 390 400 410 420 HCDRSPDLRT VGAKLKDILG VQNTSLKTRR LVVDVCDIVA KRAAHLAAAG IHGILKKLGR 430 440 450 460 470 480 DVPNTDKQRT VIAVDGGLYE HYTIFAECVE STLRDMLGED VSSTIVIKLA KDGSGIGAAL 490 LAAAHSQYRE AEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)." Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H., Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S. Planta 224:598-611(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, NOMENCLATURE. Strain: cv. Jinmi.
[2]	"The hexokinase gene family in rice." Wang Y.D., Cheng W., Wang X.S., Zhou X.J. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Zhonghua 15. Tissue: Flower.
[3]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ116384 mRNA. Translation: AAZ93619.1. AY884166 mRNA. Translation: AAX68419.1. AC121365 Genomic DNA. Translation: AAT47078.1. Sequence problems.
3D structure databases
ProteinModelPortal	Q2KNB9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g45590.1; LOC_Os05g45590.1; LOC_Os05g45590.
KEGG	dosa:Os05t0532600-01.
Organism-specific databases
Gramene	Q2KNB9.
Phylogenomic databases
eggNOG	COG5026.
HOGENOM	HOG000162670.
OMA	ANDCANV.
Enzyme and pathway databases
BRENDA	2.7.1.1. 4460.
Gene expression databases
ArrayExpress	Q2KNB9.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR022673. Hexokinase_C. IPR022672. Hexokinase_N. [Graphical view]
PANTHER	PTHR19443. PTHR19443. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK2_ORYSJ

Accession

Primary (citable) accession number: Q2KNB9
Secondary accession number(s): Q6I5H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 7, 2006
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program