Q2KNB7 (HXK9_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hexokinase-9
EC=2.7.1.1

Alternative name(s):
Hexokinase-5

Gene names

Name:	HXK9
Synonyms:	HXK5
Ordered Locus Names:	Os01g0722700, LOC_Os01g52450
ORF Names:	P0690B02.34

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from plastids to cytosol By similarity. Ref.1
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Plastid › chloroplast outer membrane; Single-pass membrane protein By similarity.
Tissue specificity	Expressed in roots, leaves, flowers, immature seeds, endosperm and seed coat. Ref.1
Developmental stage	Expressed during flower development until 15 days after flowering. Ref.1
Induction	Not induced by glucose or fructose treatment in leaves. Ref.1
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Membrane Plastid Plastid outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Hexokinase-9		PRO_0000247572
Regions
	Transmembrane	5 – 24	20	Helical; Potential
	Nucleotide binding	103 – 108	6	ATP Potential
	Region	173 – 199	27	Glucose-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2KNB7 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 5CB37FFE799ED223	FASTA	502	54,496
	10 20 30 40 50 60 MRKAAALASA AMAAAAVAVV STVLHQRQRR AAKRSERAEA VLLRDLQERC AAPVELLRQV 70 80 90 100 110 120 ADAMAAEMRA GLAAEGGSDL QMLVTYVDSL PSGGEKGMFY ALDLGGTNFR VLRVQLGGKE 130 140 150 160 170 180 RRIIKQDSEG ISIPQHLMSS SSHELFDFVA VALAKFVASE GEDCHLPEGT QRELGFTFSF 190 200 210 220 230 240 PVKQKSLASG TLIKWTKSFA IDEMVGKDVV AELNMAIRSQ GLDMKVTALV NDTVGTLAAG 250 260 270 280 290 300 RYVNHDTIAA VILGTGSNAA YIDHADAIPK WHGSLPKSGN MVINMEWGNF KSSHLPLTEF 310 320 330 340 350 360 DQELDAESLN PGKQVYEKSI SGMYMGELVR RILLKMAQET RIFGDNIPPK LERPYILRTL 370 380 390 400 410 420 DMLIMHHDTS SDLRTVANKL KEVLGIEYTS FTTRKLVLDV CEAIATRGAR LAAAGIYGII 430 440 450 460 470 480 QKLGQHSDSP STRRSVIAVD GGVYKYYTFF SQCMESTLSD MLGQELAPSV MIKHVNDGSG 490 500 VGAALLAASY SQYHQAESAD SS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)." Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H., Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S. Planta 224:598-611(2006) [PubMed: 16552590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, NOMENCLATURE. Strain: cv. Jinmi.
[2]	"The hexokinase gene family in rice." Wang Y.D., Cheng W., Wang X.S., Zhou X.J. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Zhonghua 15. Tissue: Flower.
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ116391 mRNA. Translation: AAZ93626.1. AY884168 mRNA. Translation: AAX68421.1. AP003292 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	Q2KNB7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KNB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
NMPDR	fig|39947.1.peg.8774.
Organism-specific databases
Gramene	Q2KNB7.
Phylogenomic databases
OMA	SAHTICK.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR022673. Hexokinase_C. IPR022672. Hexokinase_N. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK9_ORYSJ

Accession

Primary (citable) accession number: Q2KNB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 7, 2006
Last modified:	September 21, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program