Reviewed, UniProtKB/Swiss-Prot Q2KNB5 (HXK10_ORYSJ)

Names and origin

Protein names

Recommended name:
    Hexokinase-10
    EC=2.7.1.1
Alternative name(s):
    Hexokinase-7

Gene names

Name:	HXK10
Synonyms:	HXK7
Ordered Locus Names:	Os05g0375100, LOC_Os05g31110
ORF Names:	OJ1005_E12.7

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from plastids to cytosol By similarity. Ref.1
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Plastid › chloroplast outer membrane; Single-pass membrane protein By similarity.
Tissue specificity	Expressed specifically in stamen. Ref.1
Miscellaneous	Expression may pollen-specific.
Sequence similarities	Belongs to the hexokinase family.
Sequence caution	The sequence AAT01343.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Membrane Plastid Plastid outer membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Hexokinase-10		PRO_0000247573
Regions
	Transmembrane	7 – 29	23	Potential
	Nucleotide binding	105 – 110	6	ATP Potential
	Region	169 – 195	27	Glucose-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2KNB5-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 8DA351554956D7C9	FASTA	504	54,507
	10 20 30 40 50 60 MEGRAAGWVR VAAVGWAVAA CAVAAGMVAR RGAARVRWNR AVAVVRDLEE RCATPAELLQ 70 80 90 100 110 120 RVVNSLAIEM FAGLASDGGS KVRMLLTCVD ALPDGSEEGI SYAIDLGGTS FRVLKVELGA 130 140 150 160 170 180 GSTIINRKVE HQPIPENLTK GTSDDLFNFI ASALKNFIER EGGEVEGRAL GFTFSFPVRQ 190 200 210 220 230 240 TSISSGTLIR WTKEFSIEEA VGKDVAQCLN EALARNGLNM KVNVLVNNTV GTLALGHYYD 250 260 270 280 290 300 DDTVAAVIIG AGTNACYIER NDAIIKSLGR VTNSERTVVN VEWGSFRPPQ IELTPYDICF 310 320 330 340 350 360 NNETWNYYDQ GFEKMISGVY LGEIARLVFQ KMAEESDIFG TAVDGLSTPF VLSTPNLAAI 370 380 390 400 410 420 REDDSPDLRE VGKILEEHLK LPDVPLKTRK LVARVSDIIT RRAARLAAAA IVAILQKIGC 430 440 450 460 470 480 DGTLCGSTQV RTMRGVRRRT VVAIEGGLFE GYSVFREYLN EALVEILGEE IAATVSLRVM 490 500 EEGSGTGAAL LAAAYSSARQ KNSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)." Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H., Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S. Planta 224:598-611(2006) [PubMed: 16552590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, NOMENCLATURE. Strain: cv. Jinmi.
[2]	"The hexokinase gene family in rice." Wang Y.D., Cheng W., Wang X.S., Zhou X.J. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Zhonghua 15. Tissue: Flower.
[3]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ116392 mRNA. Translation: AAZ93627.1. AY884170 mRNA. Translation: AAX68423.1. AC108874 Genomic DNA. Translation: AAT01343.1. Sequence problems.
RefSeq	NP_001055376.1.
UniGene	Os.74778
3D structure databases
SMR	Q2KNB5. Positions 44-502.
ModBase	Search...
Genome annotation databases
GeneID	4338609.
KEGG	osa:4338609.
Organism-specific databases
Gramene	Q2KNB5.
Phylogenomic databases
PhylomeDB	Q2KNB5.
Enzyme and pathway databases
BRENDA	2.7.1.1. 1975.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK10_ORYSJ

Accession

Primary (citable) accession number: Q2KNB5
Secondary accession number(s): Q75K74

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 7, 2006
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)