Reviewed, UniProtKB/Swiss-Prot Q2KNB4 (HXK3_ORYSJ)

Names and origin

Protein names

Recommended name:
    Hexokinase-3
    EC=2.7.1.1
Alternative name(s):
    Hexokinase-8

Gene names

Name:	HXK3
Synonyms:	HXK8
Ordered Locus Names:	Os01g0940100, LOC_Os01g71320
ORF Names:	B1150F11.26-1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol By similarity.
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Tissue specificity	Expressed in roots, leaves, flowers, immature seeds and seed coat. Ref.1
Developmental stage	Expressed during flower development until 8 days after flowering. Ref.1
Induction	Not induced by glucose or fructose treatment in leaves. Ref.1
Sequence similarities	Belongs to the hexokinase family.
Sequence caution	The sequence BAD87613.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Hexokinase-3		PRO_0000247566
Regions
	Transmembrane	4 – 24	21	Potential
	Nucleotide binding	101 – 106	6	ATP Potential
	Region	165 – 191	27	Glucose-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2KNB4-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 0179231A6AC47A2C	FASTA	500	53,794
	10 20 30 40 50 60 MGRVGLGVAV GCAAVTCAIA AALVARRASA RARWRRAVAL LREFEEGCAT PPARLRQVVD 70 80 90 100 110 120 AMVVEMHAGL ASDGGSKLKM LLTFVDALPS GSEEGVYYSI DLGGTNFRVL RVQVGAGSVI 130 140 150 160 170 180 VNQKVEQQPI PEELTKGTTE GLFNFVALAL KNFLEGEDDQ DGKMALGFTF SFPVRQISVS 190 200 210 220 230 240 SGSLIRWTKG FSIRDTVGRD VAQCLNEALA NCGLNVRVTA LVNDTVGTLA LGHYYDEDTV 250 260 270 280 290 300 AAVIIGSGTN ACYIERTDAI IKCQGLLTNS GGMVVNMEWG NFWSSHLPRT PYDILLDDET 310 320 330 340 350 360 HNRNDQGFEK MISGMYLGEI ARLVFHRMAQ ESDVFGDAAD SLSNPFILST PFLAAIREDD 370 380 390 400 410 420 SPDLSEVRRI LREHLKIPDA PLKTRRLVVK VCDIVTRRAA RLAAAGIVGI LKKLGRDGSG 430 440 450 460 470 480 AASSGRGRGQ PRRTVVAIEG GLYQGYPVFR EYLDEALVEI LGEEVARNVT LRVTEDGSGV 490 500 GAALLAAVHS SNRQQQGGPI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)." Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H., Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S. Planta 224:598-611(2006) [PubMed: 16552590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, NOMENCLATURE. Strain: cv. Jinmi.
[2]	"The hexokinase gene family in rice." Wang Y.D., Cheng W., Wang X.S., Zhou X.J. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Zhonghua 15. Tissue: Flower.
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ116385 mRNA. Translation: AAZ93620.1. AY884171 mRNA. Translation: AAX68424.1. AP003412 Genomic DNA. Translation: BAD87613.1. Sequence problems.
3D structure databases
ModBase	Search...
Organism-specific databases
Gramene	Q2KNB4.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProDom	PD001109. Hexokinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK3_ORYSJ

Accession

Primary (citable) accession number: Q2KNB4
Secondary accession number(s): Q5JLP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 7, 2006
Last modified:	October 13, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)