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Q2KMM4

- LX12B_RAT

UniProt

Q2KMM4 - LX12B_RAT

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Protein

Arachidonate 12-lipoxygenase, 12R-type

Gene

Alox12b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.1 Publication

Catalytic activityi

Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.1 Publication

Cofactori

Binds 1 iron ion per subunit.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi398 – 3981Iron; catalyticPROSITE-ProRule annotation
Metal bindingi403 – 4031Iron; catalyticPROSITE-ProRule annotation
Metal bindingi578 – 5781Iron; catalyticPROSITE-ProRule annotation
Metal bindingi582 – 5821Iron; catalyticPROSITE-ProRule annotation
Metal bindingi701 – 7011Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  1. arachidonate 12-lipoxygenase activity Source: UniProtKB
  2. iron ion binding Source: InterPro
  3. linoleate 9S-lipoxygenase activity Source: UniProtKB

GO - Biological processi

  1. arachidonic acid metabolic process Source: UniProtKB
  2. ceramide biosynthetic process Source: UniProtKB
  3. establishment of skin barrier Source: UniProtKB
  4. hepoxilin biosynthetic process Source: UniProtKB
  5. linoleic acid metabolic process Source: UniProtKB
  6. lipoxygenase pathway Source: UniProtKB
  7. oxidation-reduction process Source: UniProtKB
  8. positive regulation of gene expression Source: UniProtKB
  9. positive regulation of MAPK cascade Source: UniProtKB
  10. positive regulation of mucus secretion Source: UniProtKB
  11. protein lipidation Source: UniProtKB
  12. sphingolipid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00222.
UPA00881.

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 12-lipoxygenase, 12R-type (EC:1.13.11.-)
Short name:
12R-LOX
Short name:
12R-lipoxygenase
Alternative name(s):
Epidermis-type lipoxygenase 12
Gene namesi
Name:Alox12bImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi1305330. Alox12b.

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 701701Arachidonate 12-lipoxygenase, 12R-typePRO_0000244486Add
BLAST

Proteomic databases

PRIDEiQ2KMM4.

Expressioni

Gene expression databases

GenevestigatoriQ2KMM4.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033009.

Structurei

3D structure databases

ProteinModelPortaliQ2KMM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 119118PLATPROSITE-ProRule annotationAdd
BLAST
Domaini120 – 701582LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Sequence Analysis
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG69653.
GeneTreeiENSGT00550000074415.
HOGENOMiHOG000234358.
HOVERGENiHBG005150.
InParanoidiQ2KMM4.
KOiK08021.
OrthoDBiEOG7V49XR.
PhylomeDBiQ2KMM4.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KMM4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATYKVKVAT GTDFFSGTLD SISLTIVGTQ GESHKQRLNH FGRDFATGAV
60 70 80 90 100
DDYTVQCQQD LGELIIIRLH KEPHSFLPKD PWYCNYVQIC APNCRVYHFP
110 120 130 140 150
AYQWMDGYET LSLREATGKT TADDTLPILL EHRQEEIRAK KDFYHWRVFV
160 170 180 190 200
PGLPNYVDIP SYHPPPRRCR NPNRPEWNGY IPGFPILINI KATRFLNLNL
210 220 230 240 250
RFSFVKTASF FYRLGPMALA FKLRGLVDRK RSWKRLKDIK NIFPATKTVV
260 270 280 290 300
SEYVAEHWTE DSFFGYQYLN GINPGHIRRC MQIPDKFPVT DEMVAPFLGE
310 320 330 340 350
GTCLQAELEK GNIYLADYRI LDGIPTVELN GQKQHHCAPI CLLHFGPDGN
360 370 380 390 400
MMPIAIQLSQ TPGPDCPIFL PNDSEWDWLL AKTWVRYAEF YSHEAVAHLL
410 420 430 440 450
ESHLIGEAFC LALLRNLPMC HPLYKLLIPH TRYNVQINSI GRALLLNKGG
460 470 480 490 500
LSARAMSLGL EGFAQVMVRG LSELTYKSLC IPNDFVERGV QDLPGYYFRD
510 520 530 540 550
DSLAVWYAME RYVTEIITYY YPNDAAVEGD PELQCWVQEI FKECLLERES
560 570 580 590 600
SGFPTCLRTV PELIEYVTMV MYTCSARHAA VNTGQLEYTS WMPNFPSSMR
610 620 630 640 650
NPPMQSKGLT TLQTFMDTLP DVKTTCIVLL VLWTLCREPD DRRPLGHFPD
660 670 680 690 700
IHFVEEAPRR SMEAFRQNLN QISHNIRQRN KCLNLPYYYL DPVLIENSIS

I
Length:701
Mass (Da):80,741
Last modified:March 7, 2006 - v1
Checksum:i6BE209D98DEB4DB9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti276 – 2761H → L in AAX85362. (PubMed:15222128)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY903231 mRNA. Translation: AAX85361.1.
AY903232 mRNA. Translation: AAX85362.1.
AY903233 mRNA. Translation: AAX85363.1.
RefSeqiNP_001034466.1. NM_001039377.1.
UniGeneiRn.160588.

Genome annotation databases

EnsembliENSRNOT00000039535; ENSRNOP00000033009; ENSRNOG00000022210.
GeneIDi287425.
KEGGirno:287425.
UCSCiRGD:1305330. rat.

Cross-referencesi

Web resourcesi

Protein Spotlight

about water - Issue 153 of September 2013

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY903231 mRNA. Translation: AAX85361.1 .
AY903232 mRNA. Translation: AAX85362.1 .
AY903233 mRNA. Translation: AAX85363.1 .
RefSeqi NP_001034466.1. NM_001039377.1.
UniGenei Rn.160588.

3D structure databases

ProteinModelPortali Q2KMM4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000033009.

Proteomic databases

PRIDEi Q2KMM4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000039535 ; ENSRNOP00000033009 ; ENSRNOG00000022210 .
GeneIDi 287425.
KEGGi rno:287425.
UCSCi RGD:1305330. rat.

Organism-specific databases

CTDi 242.
RGDi 1305330. Alox12b.

Phylogenomic databases

eggNOGi NOG69653.
GeneTreei ENSGT00550000074415.
HOGENOMi HOG000234358.
HOVERGENi HBG005150.
InParanoidi Q2KMM4.
KOi K08021.
OrthoDBi EOG7V49XR.
PhylomeDBi Q2KMM4.

Enzyme and pathway databases

UniPathwayi UPA00222 .
UPA00881 .

Miscellaneous databases

NextBioi 626105.
PROi Q2KMM4.

Gene expression databases

Genevestigatori Q2KMM4.

Family and domain databases

Gene3Di 2.60.60.20. 1 hit.
InterProi IPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view ]
PANTHERi PTHR11771. PTHR11771. 1 hit.
Pfami PF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view ]
PRINTSi PR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTi SM00308. LH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEi PS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular analysis of the sex hormone-binding globulin gene in the rat hypodactylous mutation (Hd)."
    Liska F., Goesele C., Kren V., Huebner N., Krenova D.
    Folia Biol. (Praha) 50:63-68(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Brown Norway/Cub, SHR/OlaIpcvImported and Wistar Hd.
    Tissue: TestisImported.
  2. "Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia."
    Gregus A.M., Dumlao D.S., Wei S.C., Norris P.C., Catella L.C., Meyerstein F.G., Buczynski M.W., Steinauer J.J., Fitzsimmons B.L., Yaksh T.L., Dennis E.A.
    FASEB J. 27:1939-1949(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiLX12B_RAT
AccessioniPrimary (citable) accession number: Q2KMM4
Secondary accession number(s): Q2KMM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 7, 2006
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3