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Protein

Arachidonate 12-lipoxygenase, 12R-type

Gene

Alox12b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.1 Publication

Catalytic activityi

Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.1 Publication

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi: hydroperoxy eicosatetraenoic acid biosynthesis

This protein is involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis and in Lipid metabolism.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi398Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi403Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi578Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi582Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi701Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandIron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00222
UPA00881

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 12-lipoxygenase, 12R-type (EC:1.13.11.-)
Short name:
12R-LOX
Short name:
12R-lipoxygenase
Alternative name(s):
Epidermis-type lipoxygenase 12
Gene namesi
Name:Alox12bImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1305330 Alox12b

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002444861 – 701Arachidonate 12-lipoxygenase, 12R-typeAdd BLAST701

Proteomic databases

PaxDbiQ2KMM4
PRIDEiQ2KMM4

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033009

Structurei

3D structure databases

ProteinModelPortaliQ2KMM4
SMRiQ2KMM4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 119PLATPROSITE-ProRule annotationAdd BLAST118
Domaini120 – 701LipoxygenasePROSITE-ProRule annotationAdd BLAST582

Sequence similaritiesi

Belongs to the lipoxygenase family.Sequence analysis

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
HOGENOMiHOG000234358
HOVERGENiHBG005150
InParanoidiQ2KMM4
KOiK08021
PhylomeDBiQ2KMM4

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q2KMM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATYKVKVAT GTDFFSGTLD SISLTIVGTQ GESHKQRLNH FGRDFATGAV
60 70 80 90 100
DDYTVQCQQD LGELIIIRLH KEPHSFLPKD PWYCNYVQIC APNCRVYHFP
110 120 130 140 150
AYQWMDGYET LSLREATGKT TADDTLPILL EHRQEEIRAK KDFYHWRVFV
160 170 180 190 200
PGLPNYVDIP SYHPPPRRCR NPNRPEWNGY IPGFPILINI KATRFLNLNL
210 220 230 240 250
RFSFVKTASF FYRLGPMALA FKLRGLVDRK RSWKRLKDIK NIFPATKTVV
260 270 280 290 300
SEYVAEHWTE DSFFGYQYLN GINPGHIRRC MQIPDKFPVT DEMVAPFLGE
310 320 330 340 350
GTCLQAELEK GNIYLADYRI LDGIPTVELN GQKQHHCAPI CLLHFGPDGN
360 370 380 390 400
MMPIAIQLSQ TPGPDCPIFL PNDSEWDWLL AKTWVRYAEF YSHEAVAHLL
410 420 430 440 450
ESHLIGEAFC LALLRNLPMC HPLYKLLIPH TRYNVQINSI GRALLLNKGG
460 470 480 490 500
LSARAMSLGL EGFAQVMVRG LSELTYKSLC IPNDFVERGV QDLPGYYFRD
510 520 530 540 550
DSLAVWYAME RYVTEIITYY YPNDAAVEGD PELQCWVQEI FKECLLERES
560 570 580 590 600
SGFPTCLRTV PELIEYVTMV MYTCSARHAA VNTGQLEYTS WMPNFPSSMR
610 620 630 640 650
NPPMQSKGLT TLQTFMDTLP DVKTTCIVLL VLWTLCREPD DRRPLGHFPD
660 670 680 690 700
IHFVEEAPRR SMEAFRQNLN QISHNIRQRN KCLNLPYYYL DPVLIENSIS

I
Length:701
Mass (Da):80,741
Last modified:March 7, 2006 - v1
Checksum:i6BE209D98DEB4DB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti276H → L in AAX85362 (PubMed:15222128).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY903231 mRNA Translation: AAX85361.1
AY903232 mRNA Translation: AAX85362.1
AY903233 mRNA Translation: AAX85363.1
RefSeqiNP_001034466.1, NM_001039377.1
UniGeneiRn.160588

Genome annotation databases

GeneIDi287425
KEGGirno:287425
UCSCiRGD:1305330 rat

Similar proteinsi

Entry informationi

Entry nameiLX12B_RAT
AccessioniPrimary (citable) accession number: Q2KMM4
Secondary accession number(s): Q2KMM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 7, 2006
Last modified: March 28, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome