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Protein

Arachidonate 12-lipoxygenase, 12R-type

Gene

Alox12b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.1 Publication

Catalytic activityi

Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12R)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.1 Publication

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi398 – 3981Iron; catalyticPROSITE-ProRule annotation
Metal bindingi403 – 4031Iron; catalyticPROSITE-ProRule annotation
Metal bindingi578 – 5781Iron; catalyticPROSITE-ProRule annotation
Metal bindingi582 – 5821Iron; catalyticPROSITE-ProRule annotation
Metal bindingi701 – 7011Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_303224. Synthesis of 12-eicosatetraenoic acid derivatives.
UniPathwayiUPA00222.
UPA00881.

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 12-lipoxygenase, 12R-type (EC:1.13.11.-)
Short name:
12R-LOX
Short name:
12R-lipoxygenase
Alternative name(s):
Epidermis-type lipoxygenase 12
Gene namesi
Name:Alox12bImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1305330. Alox12b.

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 701701Arachidonate 12-lipoxygenase, 12R-typePRO_0000244486Add
BLAST

Proteomic databases

PRIDEiQ2KMM4.

Expressioni

Gene expression databases

GenevestigatoriQ2KMM4.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033009.

Structurei

3D structure databases

ProteinModelPortaliQ2KMM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 119118PLATPROSITE-ProRule annotationAdd
BLAST
Domaini120 – 701582LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Sequence Analysis
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG69653.
HOGENOMiHOG000234358.
HOVERGENiHBG005150.
InParanoidiQ2KMM4.
KOiK08021.
OrthoDBiEOG7V49XR.
PhylomeDBiQ2KMM4.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KMM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATYKVKVAT GTDFFSGTLD SISLTIVGTQ GESHKQRLNH FGRDFATGAV
60 70 80 90 100
DDYTVQCQQD LGELIIIRLH KEPHSFLPKD PWYCNYVQIC APNCRVYHFP
110 120 130 140 150
AYQWMDGYET LSLREATGKT TADDTLPILL EHRQEEIRAK KDFYHWRVFV
160 170 180 190 200
PGLPNYVDIP SYHPPPRRCR NPNRPEWNGY IPGFPILINI KATRFLNLNL
210 220 230 240 250
RFSFVKTASF FYRLGPMALA FKLRGLVDRK RSWKRLKDIK NIFPATKTVV
260 270 280 290 300
SEYVAEHWTE DSFFGYQYLN GINPGHIRRC MQIPDKFPVT DEMVAPFLGE
310 320 330 340 350
GTCLQAELEK GNIYLADYRI LDGIPTVELN GQKQHHCAPI CLLHFGPDGN
360 370 380 390 400
MMPIAIQLSQ TPGPDCPIFL PNDSEWDWLL AKTWVRYAEF YSHEAVAHLL
410 420 430 440 450
ESHLIGEAFC LALLRNLPMC HPLYKLLIPH TRYNVQINSI GRALLLNKGG
460 470 480 490 500
LSARAMSLGL EGFAQVMVRG LSELTYKSLC IPNDFVERGV QDLPGYYFRD
510 520 530 540 550
DSLAVWYAME RYVTEIITYY YPNDAAVEGD PELQCWVQEI FKECLLERES
560 570 580 590 600
SGFPTCLRTV PELIEYVTMV MYTCSARHAA VNTGQLEYTS WMPNFPSSMR
610 620 630 640 650
NPPMQSKGLT TLQTFMDTLP DVKTTCIVLL VLWTLCREPD DRRPLGHFPD
660 670 680 690 700
IHFVEEAPRR SMEAFRQNLN QISHNIRQRN KCLNLPYYYL DPVLIENSIS

I
Length:701
Mass (Da):80,741
Last modified:March 7, 2006 - v1
Checksum:i6BE209D98DEB4DB9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti276 – 2761H → L in AAX85362 (PubMed:15222128).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY903231 mRNA. Translation: AAX85361.1.
AY903232 mRNA. Translation: AAX85362.1.
AY903233 mRNA. Translation: AAX85363.1.
RefSeqiNP_001034466.1. NM_001039377.1.
UniGeneiRn.160588.

Genome annotation databases

GeneIDi287425.
KEGGirno:287425.
UCSCiRGD:1305330. rat.

Cross-referencesi

Web resourcesi

Protein Spotlight

about water - Issue 153 of September 2013

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY903231 mRNA. Translation: AAX85361.1.
AY903232 mRNA. Translation: AAX85362.1.
AY903233 mRNA. Translation: AAX85363.1.
RefSeqiNP_001034466.1. NM_001039377.1.
UniGeneiRn.160588.

3D structure databases

ProteinModelPortaliQ2KMM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033009.

Proteomic databases

PRIDEiQ2KMM4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi287425.
KEGGirno:287425.
UCSCiRGD:1305330. rat.

Organism-specific databases

CTDi242.
RGDi1305330. Alox12b.

Phylogenomic databases

eggNOGiNOG69653.
HOGENOMiHOG000234358.
HOVERGENiHBG005150.
InParanoidiQ2KMM4.
KOiK08021.
OrthoDBiEOG7V49XR.
PhylomeDBiQ2KMM4.

Enzyme and pathway databases

UniPathwayiUPA00222.
UPA00881.
ReactomeiREACT_303224. Synthesis of 12-eicosatetraenoic acid derivatives.

Miscellaneous databases

NextBioi626105.
PROiQ2KMM4.

Gene expression databases

GenevestigatoriQ2KMM4.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular analysis of the sex hormone-binding globulin gene in the rat hypodactylous mutation (Hd)."
    Liska F., Goesele C., Kren V., Huebner N., Krenova D.
    Folia Biol. (Praha) 50:63-68(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Brown Norway/Cub, SHR/OlaIpcvImported and Wistar Hd.
    Tissue: TestisImported.
  2. "Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia."
    Gregus A.M., Dumlao D.S., Wei S.C., Norris P.C., Catella L.C., Meyerstein F.G., Buczynski M.W., Steinauer J.J., Fitzsimmons B.L., Yaksh T.L., Dennis E.A.
    FASEB J. 27:1939-1949(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiLX12B_RAT
AccessioniPrimary (citable) accession number: Q2KMM4
Secondary accession number(s): Q2KMM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 7, 2006
Last modified: May 27, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.