Reviewed, UniProtKB/Swiss-Prot Q2KMM4 (LX12B_RAT)

Names and origin

Protein names

Recommended name:
    Arachidonate 12-lipoxygenase, 12R type
      Short name=12R-lipoxygenase
      Short name=12R-LOX
    EC=1.13.11.-
Alternative name(s):
    Epidermis-type lipoxygenase 12

Gene names

Name:

Alox12b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts arachidonic acid to 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) By similarity. UniProtKB O75342
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene D4 biosynthesis.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 701	701	Arachidonate 12-lipoxygenase, 12R type		PRO_0000244486
Regions
	Domain	2 – 119	118	PLAT
	Domain	120 – 701	582	Lipoxygenase
Sites
	Metal binding	398	1	Iron; catalytic By similarity UniProtKB P12530
	Metal binding	403	1	Iron; catalytic By similarity UniProtKB P12530
	Metal binding	578	1	Iron; catalytic By similarity UniProtKB P12530
	Metal binding	582	1	Iron; catalytic By similarity
	Metal binding	701	1	Iron; via carboxylate; catalytic By similarity UniProtKB P12530
Experimental info
	Sequence conflict	276	1	H → L in AAX85362. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q2KMM4-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 6BE209D98DEB4DB9	FASTA	701	80,741
	10 20 30 40 50 60 MATYKVKVAT GTDFFSGTLD SISLTIVGTQ GESHKQRLNH FGRDFATGAV DDYTVQCQQD 70 80 90 100 110 120 LGELIIIRLH KEPHSFLPKD PWYCNYVQIC APNCRVYHFP AYQWMDGYET LSLREATGKT 130 140 150 160 170 180 TADDTLPILL EHRQEEIRAK KDFYHWRVFV PGLPNYVDIP SYHPPPRRCR NPNRPEWNGY 190 200 210 220 230 240 IPGFPILINI KATRFLNLNL RFSFVKTASF FYRLGPMALA FKLRGLVDRK RSWKRLKDIK 250 260 270 280 290 300 NIFPATKTVV SEYVAEHWTE DSFFGYQYLN GINPGHIRRC MQIPDKFPVT DEMVAPFLGE 310 320 330 340 350 360 GTCLQAELEK GNIYLADYRI LDGIPTVELN GQKQHHCAPI CLLHFGPDGN MMPIAIQLSQ 370 380 390 400 410 420 TPGPDCPIFL PNDSEWDWLL AKTWVRYAEF YSHEAVAHLL ESHLIGEAFC LALLRNLPMC 430 440 450 460 470 480 HPLYKLLIPH TRYNVQINSI GRALLLNKGG LSARAMSLGL EGFAQVMVRG LSELTYKSLC 490 500 510 520 530 540 IPNDFVERGV QDLPGYYFRD DSLAVWYAME RYVTEIITYY YPNDAAVEGD PELQCWVQEI 550 560 570 580 590 600 FKECLLERES SGFPTCLRTV PELIEYVTMV MYTCSARHAA VNTGQLEYTS WMPNFPSSMR 610 620 630 640 650 660 NPPMQSKGLT TLQTFMDTLP DVKTTCIVLL VLWTLCREPD DRRPLGHFPD IHFVEEAPRR 670 680 690 700 SMEAFRQNLN QISHNIRQRN KCLNLPYYYL DPVLIENSIS I

References

[1]

"Molecular analysis of the sex hormone-binding globulin gene in the rat hypodactylous mutation (Hd)." Liska F., Goesele C., Kren V., Huebner N., Krenova D. Folia Biol. (Praha) 50:63-68(2004) [PubMed: 15222128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Brown Norway/Cub, SHR/OlaIpcv and Wistar Hd. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY903231 mRNA. Translation: AAX85361.1. AY903232 mRNA. Translation: AAX85362.1. AY903233 mRNA. Translation: AAX85363.1.
IPI	IPI00369660.
RefSeq	NP_001034466.1.
UniGene	Rn.160588
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000022210. Rattus norvegicus. [Contig view]
GeneID	287425.
KEGG	rno:287425.
Organism-specific databases
RGD	1305330. Alox12b.
Phylogenomic databases
HOVERGEN	Q2KMM4.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	626105.

Entry information

Entry name

LX12B_RAT

Accession

Primary (citable) accession number: Q2KMM4
Secondary accession number(s): Q2KMM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)