Reviewed, UniProtKB/Swiss-Prot Q2KJJ9 (F16P2_BOVIN)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase isozyme 2
      Short name=FBPase 2
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

Gene names

Name:

FBP2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Enzyme regulation	Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. Fructose-2,6-biphosphate acts as competitive inhibitor By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Gluconeogenesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Fructose-1,6-bisphosphatase isozyme 2		PRO_0000247323
Regions
	Nucleotide binding	18 – 22	5	AMP By similarity
	Nucleotide binding	28 – 32	5	AMP By similarity
	Nucleotide binding	113 – 114	2	AMP By similarity
	Region	122 – 125	4	Substrate binding By similarity
	Region	213 – 216	4	Substrate binding By similarity
	Region	244 – 249	6	Substrate binding By similarity
	Region	275 – 277	3	Substrate binding By similarity
Sites
	Metal binding	69	1	Magnesium 1 By similarity
	Metal binding	98	1	Magnesium 1 By similarity
	Metal binding	98	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 3 By similarity
	Metal binding	121	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	122	1	Magnesium 3 By similarity
	Metal binding	281	1	Magnesium 3 By similarity
	Binding site	141	1	AMP By similarity
	Binding site	265	1	Substrate By similarity
Amino acid modifications
	Modified residue	216	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KJJ9-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: DB3FE9856DDC5FB2	FASTA	339	36,767
	10 20 30 40 50 60 MGDRSPFETD MLTLTRYVME KGRQAKGTGE LTQLLNSMLT AIKAISSAVR KAGLANLYGI 70 80 90 100 110 120 AGSVNVTGDE VKKLDVLSNA LVINMLQSSY STCVLVSEEN KEAIITSKEK RGKYVVCFDP 130 140 150 160 170 180 LDGSSNIDCL ASIGTIFAIY RKTSEDEPSE KDALQPGRNI VAAGYALYGS ATLVALSTGQ 190 200 210 220 230 240 GVDLFMLDPA LGEFVLVEKD VKIKKKGKIY SLNEGYAKYF DAATTEYVQK KKFPEDGSAP 250 260 270 280 290 300 YGARYVGSMV ADVHRTLVYG GIFLYPANQK SPKGKLRLLY ECNPVAYIIE QAGGLATTGT 310 320 330 QPVLDVKPEA IHQRVPLILG SPEDVQEYLT CVQKNQAGR

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC102390 mRNA. Translation: AAI02391.1.
IPI	IPI00716812.
RefSeq	NP_001039629.1.
UniGene	Bt.28208
3D structure databases
SMR	Q2KJJ9. Positions 7-337.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KJJ9.
Genome annotation databases
Ensembl	ENSBTAT00000026056; ENSBTAP00000026056; ENSBTAG00000019554; Bos taurus. [Genome view]
GeneID	514066.
KEGG	bta:514066.
Organism-specific databases
CTD	514066.
Phylogenomic databases
eggNOG	maNOG17246.
HOVERGEN	Q2KJJ9.
InParanoid	Q2KJJ9.
OMA	ITAKEKR.
OrthoDB	EOG9Z3905.
PhylomeDB	Q2KJJ9.
Enzyme and pathway databases
BRENDA	3.1.3.11. 251.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16P2_BOVIN

Accession

Primary (citable) accession number: Q2KJJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 7, 2006
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)