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Q2KJI2 (STT3A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
Short name=Oligosaccharyl transferase subunit STT3A
Short name=STT3-A
EC=2.4.1.119
Gene names
Name:STT3A
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity By similarity.

Catalytic activity

Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the STT3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 705705Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
PRO_0000326556

Regions

Topological domain1 – 1717Cytoplasmic Potential
Transmembrane18 – 3821Helical; Potential
Topological domain39 – 11476Lumenal Potential
Transmembrane115 – 13521Helical; Potential
Topological domain136 – 16934Cytoplasmic Potential
Transmembrane170 – 19021Helical; Potential
Topological domain191 – 20818Lumenal Potential
Transmembrane209 – 22921Helical; Potential
Topological domain230 – 2356Cytoplasmic Potential
Transmembrane236 – 25924Helical; Potential
Topological domain260 – 30041Lumenal Potential
Transmembrane301 – 32121Helical; Potential
Topological domain322 – 35938Cytoplasmic Potential
Transmembrane360 – 38021Helical; Potential
Topological domain381 – 3833Lumenal Potential
Transmembrane384 – 40421Helical; Potential
Topological domain4051Cytoplasmic Potential
Transmembrane406 – 42621Helical; Potential
Topological domain427 – 45327Lumenal Potential
Transmembrane454 – 47421Helical; Potential
Topological domain475 – 705231Lumenal Potential

Amino acid modifications

Glycosylation3811N-linked (GlcNAc...) Potential
Glycosylation5371N-linked (GlcNAc...) Potential
Glycosylation5441N-linked (GlcNAc...) Potential
Glycosylation5481N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q2KJI2 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 2B2923DA16BC70AB

FASTA70580,656
        10         20         30         40         50         60 
MTKLGFLRLS YEKQDTLLKL LILSMAAVLS FSTRLFAVLR FESVIHEFDP YFNYRTTRFL 

        70         80         90        100        110        120 
AEEGFYKFHN WFDDRAWYPL GRIIGGTIYP GLMITSAAIY HVLHFFHITI DIRNVCVFLA 

       130        140        150        160        170        180 
PLFSSFTTIV TYHLTKELKD AGAGLLAAAM IAVVPGYISR SVAGSYDNEG IAIFCMLLTY 

       190        200        210        220        230        240 
YMWIKAVKTG SIYWAAKCAL AYFYMVSSWG GYVFLINLIP LHVLVLMLTG RFSHRIYVAY 

       250        260        270        280        290        300 
CTVYCLGTIL SMQISFVGFQ PVLSSEHMAA FGVFGLCQIH AFVDYLRSKL NPQQFEVLFR 

       310        320        330        340        350        360 
SVISLVGFVL LTIGALLMLT GKISPWTGRF YSLLDPSYAK NNIPIIASVS EHQPTTWSSY 

       370        380        390        400        410        420 
YFDLQLLVFM FPVGLYYCFS NLSDARIFII MYGVTSMYFS AVMVRLMLVL APVMCILSGI 

       430        440        450        460        470        480 
GVSQVLSTYM KNLDISRQDK KSKKQQDSTY PIKNEVASGM ILVMAFFLIT YTFHSTWVTS 

       490        500        510        520        530        540 
EAYSSPSIVL SARGGDGSRI IFDDFREAYY WLRHNTPEDA KVMSWWDYGY QITAMANRTI 

       550        560        570        580        590        600 
LVDNNTWNNT HISRVGQAMA STEEKAYEIM RELDVSYVLV IFGGLTGYSS DDINKFLWMV 

       610        620        630        640        650        660 
RIGGSTDTGK HIKEHDYYTP TGEFRVDREG SPVLLNCLMY KMCYYRFGQV YTEAKRPLGY 

       670        680        690        700 
DRVRNAEIGN KDFELDVLEE AYTTEHWLVR IYKVKDLDNR GLSRT

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References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Ileum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC105328 mRNA. Translation: AAI05329.1.
IPIIPI00697459.
RefSeqNP_001039445.1. NM_001045980.1.
UniGeneBt.21376.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ2KJI2.

Protein family/group databases

CAZyGT66. Glycosyltransferase Family 66.

Proteomic databases

PRIDEQ2KJI2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000009477; ENSBTAP00000009477; ENSBTAG00000007203.
GeneID507815.
KEGGbta:507815.

Organism-specific databases

CTD3703.

Phylogenomic databases

eggNOGmaNOG07341.
GeneTreeENSGT00390000015238.
HOVERGENHBG010606.
InParanoidQ2KJI2.
OMASHRIYIA.
OrthoDBEOG4SF95B.
PhylomeDBQ2KJI2.

Family and domain databases

InterProIPR003674. Oligo_trans_STT3.
[Graphical view]
KOK07151.
PfamPF02516. STT3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSTT3A_BOVIN
AccessionPrimary (citable) accession number: Q2KJI2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 7, 2006
Last modified: December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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