Q2KJE5 (G3PT_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific
EC=1.2.1.12

Alternative name(s):
Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:

GAPDHS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility By similarity.
Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Glyceraldehyde-3-phosphate dehydrogenase, testis-specific		PRO_0000286175
Regions
	Nucleotide binding	72 – 73	2	NAD By similarity
	Region	210 – 212	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	270 – 271	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	211	1	Nucleophile By similarity
	Binding site	93	1	NAD By similarity
	Binding site	138	1	NAD; via carbonyl oxygen By similarity
	Binding site	241	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	293	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	375	1	NAD By similarity
	Site	238	1	Activates thiol group during catalysis By similarity
Amino acid modifications
	Modified residue	305	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KJE5 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 8DF90EBDAB156160	FASTA	395	43,288
	10 20 30 40 50 60 MSKRDIVLTN VTVVQLLRQP CPEPRVEAEP EPPAQPQPQP EPIKEEVPPP PPPPPAPKKV 70 80 90 100 110 120 RELIVGINGF GRIGRLVLRA CMEKGVKVVA VNDPFIDLEY MVYMFKYDST HGRYKGNVEH 130 140 150 160 170 180 KKGQLVVDNN EISVFQCKQP KEIPWKSVGS PFVVEATGVY LSLEETKAHI EAGAQRVVIC 190 200 210 220 230 240 APSPDAPMFV MGVNEKEYNP SSMKIVSNAS CTTNCLAPLA KVIHERFGIL EGLMTTVHSY 250 260 270 280 290 300 TATQKTVDGP SKKAWRDGRG AHQNIIPAST GAAKAVGKVI PDLKGKLTGM AFRVPTPDVS 310 320 330 340 350 360 VVDLTCRLAQ PTPYSAIKDA IKAAAKGPMA GILAYTEDEV VSTDFLSDTH SSIFDAKAGI 370 380 390 ALNDNFVKLI SWYDNEYGYS NRVVDLVRYM FSRDK

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC105381 mRNA. Translation: AAI05382.1.
IPI	IPI00715799.
RefSeq	NP_001035642.1. NM_001040552.1.
UniGene	Bt.91447.
3D structure databases
HSSP	HSSP built from PDB template 1U8F based on UniProtKB P04406.
ProteinModelPortal	Q2KJE5.
SMR	Q2KJE5. Positions 65-395.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KJE5.
Proteomic databases
PRIDE	Q2KJE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000021166; ENSBTAP00000021166; ENSBTAG00000015917.
GeneID	532231.
KEGG	bta:532231.
Organism-specific databases
CTD	26330.
Phylogenomic databases
eggNOG	maNOG16805.
GeneTree	ENSGT00550000074301.
HOVERGEN	HBG000227.
InParanoid	Q2KJE5.
OrthoDB	EOG4Q84XS.
PhylomeDB	Q2KJE5.
Family and domain databases
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K10705.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PT_BOVIN

Accession

Primary (citable) accession number: Q2KJE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 7, 2006
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program