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Protein

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific

Gene

GAPDHS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS)
  2. Phosphoglycerate kinase 1 (PGK1)
  3. no protein annotated in this organism
  4. Beta-enolase (ENO3), Alpha-enolase (ENO1)
  5. Pyruvate kinase (PKM2), Pyruvate kinase (PKLR), Pyruvate kinase (PKM2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931NADBy similarity
Binding sitei138 – 1381NAD; via carbonyl oxygenBy similarity
Active sitei211 – 2111NucleophilePROSITE-ProRule annotation
Sitei238 – 2381Activates thiol group during catalysisBy similarity
Binding sitei241 – 2411Glyceraldehyde 3-phosphateBy similarity
Binding sitei293 – 2931Glyceraldehyde 3-phosphateBy similarity
Binding sitei375 – 3751NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-BTA-70171. Glycolysis.
R-BTA-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (EC:1.2.1.12)
Alternative name(s):
Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:GAPDHS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Glyceraldehyde-3-phosphate dehydrogenase, testis-specificPRO_0000286175Add
BLAST

Proteomic databases

PaxDbiQ2KJE5.
PeptideAtlasiQ2KJE5.
PRIDEiQ2KJE5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000015917.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021166.

Structurei

3D structure databases

ProteinModelPortaliQ2KJE5.
SMRiQ2KJE5. Positions 65-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6060Testis-specific N-terminal extensionBy similarityAdd
BLAST
Regioni210 – 2123Glyceraldehyde 3-phosphate bindingBy similarity
Regioni270 – 2712Glyceraldehyde 3-phosphate bindingBy similarity

Domaini

The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
HOGENOMiHOG000071678.
HOVERGENiHBG000227.
InParanoidiQ2KJE5.
KOiK10705.
OMAiHSLYQNQ.
OrthoDBiEOG091G0B1Y.
TreeFamiTF300533.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KJE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRDIVLTN VTVVQLLRQP CPEPRVEAEP EPPAQPQPQP EPIKEEVPPP
60 70 80 90 100
PPPPPAPKKV RELIVGINGF GRIGRLVLRA CMEKGVKVVA VNDPFIDLEY
110 120 130 140 150
MVYMFKYDST HGRYKGNVEH KKGQLVVDNN EISVFQCKQP KEIPWKSVGS
160 170 180 190 200
PFVVEATGVY LSLEETKAHI EAGAQRVVIC APSPDAPMFV MGVNEKEYNP
210 220 230 240 250
SSMKIVSNAS CTTNCLAPLA KVIHERFGIL EGLMTTVHSY TATQKTVDGP
260 270 280 290 300
SKKAWRDGRG AHQNIIPAST GAAKAVGKVI PDLKGKLTGM AFRVPTPDVS
310 320 330 340 350
VVDLTCRLAQ PTPYSAIKDA IKAAAKGPMA GILAYTEDEV VSTDFLSDTH
360 370 380 390
SSIFDAKAGI ALNDNFVKLI SWYDNEYGYS NRVVDLVRYM FSRDK
Length:395
Mass (Da):43,288
Last modified:March 7, 2006 - v1
Checksum:i8DF90EBDAB156160
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105381 mRNA. Translation: AAI05382.1.
RefSeqiNP_001035642.1. NM_001040552.2.
UniGeneiBt.91447.

Genome annotation databases

EnsembliENSBTAT00000021166; ENSBTAP00000021166; ENSBTAG00000015917.
GeneIDi532231.
KEGGibta:532231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105381 mRNA. Translation: AAI05382.1.
RefSeqiNP_001035642.1. NM_001040552.2.
UniGeneiBt.91447.

3D structure databases

ProteinModelPortaliQ2KJE5.
SMRiQ2KJE5. Positions 65-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021166.

Proteomic databases

PaxDbiQ2KJE5.
PeptideAtlasiQ2KJE5.
PRIDEiQ2KJE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000021166; ENSBTAP00000021166; ENSBTAG00000015917.
GeneIDi532231.
KEGGibta:532231.

Organism-specific databases

CTDi26330.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
HOGENOMiHOG000071678.
HOVERGENiHBG000227.
InParanoidiQ2KJE5.
KOiK10705.
OMAiHSLYQNQ.
OrthoDBiEOG091G0B1Y.
TreeFamiTF300533.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
ReactomeiR-BTA-70171. Glycolysis.
R-BTA-70263. Gluconeogenesis.

Gene expression databases

BgeeiENSBTAG00000015917.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PT_BOVIN
AccessioniPrimary (citable) accession number: Q2KJE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: March 7, 2006
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.