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Protein

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific

Gene

GAPDHS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS)
  2. Phosphoglycerate kinase, Phosphoglycerate kinase (PGK1), Phosphoglycerate kinase 1 (PGK1), Phosphoglycerate kinase (PGK2), Phosphoglycerate kinase (PGK1)
  3. no protein annotated in this organism
  4. Beta-enolase (ENO3), Alpha-enolase (ENO1)
  5. Pyruvate kinase (PKM2), Pyruvate kinase (PKLR), Pyruvate kinase (PKM2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93NADBy similarity1
Binding sitei138NAD; via carbonyl oxygenBy similarity1
Active sitei211NucleophilePROSITE-ProRule annotation1
Sitei238Activates thiol group during catalysisBy similarity1
Binding sitei241Glyceraldehyde 3-phosphateBy similarity1
Binding sitei293Glyceraldehyde 3-phosphateBy similarity1
Binding sitei375NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD

Enzyme and pathway databases

ReactomeiR-BTA-70171 Glycolysis
R-BTA-70263 Gluconeogenesis
UniPathwayiUPA00109; UER00184

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (EC:1.2.1.12)
Alternative name(s):
Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:GAPDHS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Organism-specific databases

VGNCiVGNC:29248 GAPDHS

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002861751 – 395Glyceraldehyde-3-phosphate dehydrogenase, testis-specificAdd BLAST395

Proteomic databases

PaxDbiQ2KJE5
PeptideAtlasiQ2KJE5
PRIDEiQ2KJE5

Expressioni

Gene expression databases

BgeeiENSBTAG00000015917

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021166

Structurei

3D structure databases

ProteinModelPortaliQ2KJE5
SMRiQ2KJE5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 60Testis-specific N-terminal extensionBy similarityAdd BLAST60
Regioni210 – 212Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni270 – 271Glyceraldehyde 3-phosphate bindingBy similarity2

Domaini

The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657 Eukaryota
COG0057 LUCA
GeneTreeiENSGT00760000119172
HOGENOMiHOG000071678
HOVERGENiHBG000227
InParanoidiQ2KJE5
KOiK10705
OMAiDLLRYMF
OrthoDBiEOG091G0B1Y
TreeFamiTF300533

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q2KJE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRDIVLTN VTVVQLLRQP CPEPRVEAEP EPPAQPQPQP EPIKEEVPPP
60 70 80 90 100
PPPPPAPKKV RELIVGINGF GRIGRLVLRA CMEKGVKVVA VNDPFIDLEY
110 120 130 140 150
MVYMFKYDST HGRYKGNVEH KKGQLVVDNN EISVFQCKQP KEIPWKSVGS
160 170 180 190 200
PFVVEATGVY LSLEETKAHI EAGAQRVVIC APSPDAPMFV MGVNEKEYNP
210 220 230 240 250
SSMKIVSNAS CTTNCLAPLA KVIHERFGIL EGLMTTVHSY TATQKTVDGP
260 270 280 290 300
SKKAWRDGRG AHQNIIPAST GAAKAVGKVI PDLKGKLTGM AFRVPTPDVS
310 320 330 340 350
VVDLTCRLAQ PTPYSAIKDA IKAAAKGPMA GILAYTEDEV VSTDFLSDTH
360 370 380 390
SSIFDAKAGI ALNDNFVKLI SWYDNEYGYS NRVVDLVRYM FSRDK
Length:395
Mass (Da):43,288
Last modified:March 7, 2006 - v1
Checksum:i8DF90EBDAB156160
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105381 mRNA Translation: AAI05382.1
RefSeqiNP_001035642.1, NM_001040552.2
UniGeneiBt.91447

Genome annotation databases

EnsembliENSBTAT00000021166; ENSBTAP00000021166; ENSBTAG00000015917
GeneIDi532231
KEGGibta:532231

Similar proteinsi

Entry informationi

Entry nameiG3PT_BOVIN
AccessioniPrimary (citable) accession number: Q2KJE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: March 7, 2006
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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