Reviewed, UniProtKB/Swiss-Prot Q2KJC6 (METK1_BOVIN)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase isoform type-1
      Short name=AdoMet synthetase 1
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 1
      Short name=MAT 1

Gene names

Name:

MAT1A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer or homodimer By similarity.
Post-translational modification	S-nitrosylation of Cys-121 inactivates the enzyme By similarity.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
PTM	Disulfide bond S-nitrosylation
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	S-adenosylmethionine synthetase isoform type-1		PRO_0000287527
Regions
	Nucleotide binding	132 – 137	6	ATP Potential
Sites
	Metal binding	32	1	Magnesium By similarity
	Metal binding	58	1	Potassium By similarity
	Metal binding	284	1	Potassium By similarity
	Metal binding	292	1	Magnesium By similarity
	Binding site	30	1	Substrate By similarity
	Binding site	160	1	ATP Potential
	Binding site	180	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
	Binding site	250	1	Substrate; via carbonyl oxygen By similarity
	Binding site	259	1	Substrate By similarity
Amino acid modifications
	Modified residue	121	1	S-nitrosocysteine By similarity
	Disulfide bond	35 ↔ 61		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KJC6-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 2188750981D65CDB	FASTA	396	43,761
	10 20 30 40 50 60 MNGPVDGLCD HSLSEEGAFM FTSESVGEGH PDKICDQISD AVLDAHLKQD PNAKVACETV 70 80 90 100 110 120 CKTGMVLLCG EITSMAMVDY QRVVRETIQH IGYDDSAKGF DFKTCNVLVA LEQQSPDIAQ 130 140 150 160 170 180 CVHLDRNEED VGAGDQGLMF GYATDETEEC MPLTIMLAHR LNARMAELRR SGQLPWLQPD 190 200 210 220 230 240 SKTQVTVQYT QDNGAVIPMR VHTVVISVQH NEDITLEDMR RALKEQVIRA VVPARYLDED 250 260 270 280 290 300 TIYHLQPSGR FVIGGPQGDA GVTGRKIIVD TYGGWGAHGG GAFSGKDYTK VDRSAAYAAR 310 320 330 340 350 360 WVAKSLVKAG LCRRVLVQVS YAIGVAEPLS ISIFTYGTSQ KTERELLDVV NKNFDLRPGV 370 380 390 IVRDLDLKKP IYQKTACYGH FGRSEFPWEV PKKLVF

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC105410 mRNA. Translation: AAI05411.1.
IPI	IPI00716500.
RefSeq	NP_001039962.1.
UniGene	Bt.55380
3D structure databases
SMR	Q2KJC6. Positions 17-396.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KJC6.
Genome annotation databases
Ensembl	ENSBTAT00000031299; ENSBTAP00000031255; ENSBTAG00000005963; Bos taurus. [Genome view]
GeneID	541078.
KEGG	bta:541078.
Organism-specific databases
CTD	541078.
Phylogenomic databases
HOVERGEN	Q2KJC6.
OMA	LHYEKAL.
Enzyme and pathway databases
BRENDA	2.5.1.6. 251.
Family and domain databases
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK1_BOVIN

Accession

Primary (citable) accession number: Q2KJC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)