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Protein

Cell division cycle 5-like protein

Gene

CDC5L

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi31 – 54H-T-H motifPROSITE-ProRule annotationAdd BLAST24
DNA bindingi82 – 104H-T-H motifPROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, RNA-binding
Biological processCell cycle, DNA damage, DNA repair, mRNA processing, mRNA splicing, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-BTA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle 5-like protein
Short name:
Cdc5-like protein
Gene namesi
Name:CDC5L
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Organism-specific databases

VGNCiVGNC:27080 CDC5L

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002389151 – 802Cell division cycle 5-like proteinAdd BLAST802

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki135Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei227PhosphothreonineBy similarity1
Modified residuei303PhosphoserineBy similarity1
Modified residuei358PhosphoserineBy similarity1
Modified residuei377PhosphothreonineBy similarity1
Modified residuei385PhosphothreonineBy similarity1
Modified residuei396PhosphothreonineBy similarity1
Modified residuei404PhosphothreonineBy similarity1
Modified residuei411PhosphothreonineBy similarity1
Modified residuei415PhosphothreonineBy similarity1
Modified residuei417PhosphoserineBy similarity1
Modified residuei424PhosphothreonineBy similarity1
Modified residuei430PhosphothreonineBy similarity1
Modified residuei437PhosphoserineBy similarity1
Modified residuei438PhosphothreonineBy similarity1
Modified residuei442PhosphothreonineBy similarity1
Cross-linki487Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ2KJC1
PRIDEiQ2KJC1

Expressioni

Gene expression databases

BgeeiENSBTAG00000020012

Interactioni

Subunit structurei

Homodimer. Interacts with DAPK3 (By similarity). Belongs to the spliceosome complex. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1 (By similarity). Interacts with PRPF19 (via N-terminus) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026654

Structurei

3D structure databases

ProteinModelPortaliQ2KJC1
SMRiQ2KJC1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 56HTH myb-type 1PROSITE-ProRule annotationAdd BLAST56
Domaini57 – 108HTH myb-type 2PROSITE-ProRule annotationAdd BLAST52

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni200 – 206Required for interaction with CTNNBL1By similarity7
Regioni260 – 606Interaction with PPP1R8By similarityAdd BLAST347
Regioni501 – 659Interaction with DAPK3By similarityAdd BLAST159
Regioni706 – 800Interaction with PLRG1By similarityAdd BLAST95

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili142 – 245Sequence analysisAdd BLAST104
Coiled coili676 – 701Sequence analysisAdd BLAST26
Coiled coili764 – 802Sequence analysisAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi165 – 271Nuclear localization signalSequence analysisAdd BLAST107

Sequence similaritiesi

Belongs to the CEF1 family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0050 Eukaryota
COG5147 LUCA
GeneTreeiENSGT00550000074922
HOGENOMiHOG000182446
HOVERGENiHBG052766
InParanoidiQ2KJC1
KOiK12860
OMAiYKNYQKQ
OrthoDBiEOG091G0IYB
TreeFamiTF101061

Family and domain databases

CDDicd00167 SANT, 1 hit
InterProiView protein in InterPro
IPR021786 Cdc5p/Cef1
IPR009057 Homeobox-like_sf
IPR017930 Myb_dom
IPR001005 SANT/Myb
PfamiView protein in Pfam
PF11831 Myb_Cef, 1 hit
SMARTiView protein in SMART
SM00717 SANT, 2 hits
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS51294 HTH_MYB, 2 hits

Sequencei

Sequence statusi: Complete.

Q2KJC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW
60 70 80 90 100
YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPIIG RTAAQCLEHY
110 120 130 140 150
EFLLDKAAQR DNEEETTDDP RKLKPGEIDP NPETKPARPD PIDMDEDELE
160 170 180 190 200
MLSEARARLA NTQGKKAKRK AREKQLEEAR RLAALQKRRE LRAAGIEIQK
210 220 230 240 250
KRKKKRGVDY NAEIPFEKKP ALGFYDTSEE NYQTLDADFR KLRQQDLDGE
260 270 280 290 300
LRSEKEGRDR KKDKQHLKRK KESDLPSAIL QTSGVSEFTK KRSKLVLPAP
310 320 330 340 350
QISDAELQEV VKVGQASEIA RQTAEESGIT NSASSTLLSE YNVTNNSIAL
360 370 380 390 400
RTPRTPASQD RILQEAQNLM ALTNVDTPLK GGLNTPLHES DFSGVTPQRQ
410 420 430 440 450
VVQTPNTVLS TPFRTPSHGS EGLTPRSGTT PKPVINSTPG RTPLRDKLNI
460 470 480 490 500
NPEDGMADYS DPSYVKQMER ESREHLRLGL LGLPAPKNDF EIVLPENAEK
510 520 530 540 550
ELEEREIDDT YIEDAADVDA RKQAIRDAER VKEMKRMHKA VQKDLPRPSE
560 570 580 590 600
VNETILRPLN VEPPLTDLQK SEELIKKEMI TMLHYDLLHH PYEPSGNKKG
610 620 630 640 650
KTVGFGTNNA EHIAYLEHNP YEKFSKEELK KAQDVLVQEM EVVKQGMSHG
660 670 680 690 700
ELSSEAYNQV WEECYSQVLY LPGQSRYTRA NLASKKDRIE SLEKRLEINR
710 720 730 740 750
GHMTTEAKRA AKMEKKMKIL LGGYQSRAMG LMKQLNDLWD QIEQAYLELR
760 770 780 790 800
TFEELKKHED SAIPRRLECL KEDVQRQQER EKELQHRYAD LLLEKETLKA

KF
Length:802
Mass (Da):92,270
Last modified:March 7, 2006 - v1
Checksum:iD8B27B0ECEAE0A79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105417 mRNA Translation: AAI05418.1
RefSeqiNP_001070010.1, NM_001076542.1
UniGeneiBt.59498

Genome annotation databases

EnsembliENSBTAT00000026654; ENSBTAP00000026654; ENSBTAG00000020012
GeneIDi767817
KEGGibta:767817

Similar proteinsi

Entry informationi

Entry nameiCDC5L_BOVIN
AccessioniPrimary (citable) accession number: Q2KJC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 7, 2006
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health