Q2KJB5 (NOSTN_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nostrin

Alternative name(s):
Nitric oxide synthase trafficker
eNOS trafficking inducer

Gene names

Name:

NOSTRIN

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane By similarity.
Subunit structure	Homotrimer. Interacts with DAB2. Interacts with NOS3, DNM2, WASL and CAV1 By similarity.
Subcellular location	Cell membrane By similarity. Cytoplasmic vesicle. Cytoplasm Ref.2.
Tissue specificity	Present in pulmonary arterial endothelial cells (at protein level). Ref.2
Domain	The SH3 domain mediates interaction with NOS3, DNM2 and WASL By similarity. The FCH domain is necessary for membrane targeting By similarity.
Sequence similarities	Contains 1 FCH domain. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell membrane Cytoplasm Cytoplasmic vesicle Membrane
Domain	Coiled coil SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Nostrin		PRO_0000289088
Regions
	Domain	1 – 66	66	FCH
	Domain	437 – 496	60	SH3
	Coiled coil	101 – 128	28	Potential
	Coiled coil	160 – 222	63	Potential
	Coiled coil	295 – 332	38	Potential
Amino acid modifications
	Modified residue	478	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KJB5 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 3EE984572780623B	FASTA	505	57,307
	10 20 30 40 50 60 MRDPLTDCSY NKVYKNLKEF SQNGEDFCKQ ITSILQQRAN LEINYAKGLQ KLATKLSKTL 70 80 90 100 110 120 QSAKKNCLVS AWAWVSEGMK SAGDLHQKLG KAIELEAIKP AHQVLSAHEK KRKSLENEVE 130 140 150 160 170 180 KTANLVISNW NQQIKAKKKL MVSTKKHEAL FHLVESSKQI TTEKEKQKLL NKLKKSTEKL 190 200 210 220 230 240 SKEDENYYQK NVASCSTRLK WENTLENCFQ SILELEKERI QLLCNNLNQY SQHISVFGQT 250 260 270 280 290 300 LTTCHTQIHC AISKIDIEKD IQALMEETTV SSTENKSEFL LTDYFEEDPK NAMSKERQTS 310 320 330 340 350 360 SIKSKLLRLQ KDIEKASRDQ EGLERMLRAY SSHSSFSDSE SKKSTAALMD ENSLKLDLLQ 370 380 390 400 410 420 ANSYKLSSVL AELEQRPQPN HPCSNSIFKW KEKQTHSSVK ISRPVLMKRL ENVVNRASSD 430 440 450 460 470 480 GQRIPSPSST ASGVTQLGNG LCKALYPFQA RQDDELDLEK GDIVTIHKKK DEGWWFGSLK 490 500 GKKGHFPAAY VEELPLNAGD TASQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.
[2]	"Aberrant cytoplasmic sequestration of eNOS in endothelial cells after monocrotaline, hypoxia, and senescence: live-cell caveolar and cytoplasmic NO imaging." Mukhopadhyay S., Xu F., Sehgal P.B. Am. J. Physiol. 292:H1373-H1389(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC105426 mRNA. Translation: AAI05427.1.
IPI	IPI00706969.
RefSeq	NP_001039722.1. NM_001046257.2.
UniGene	Bt.17865.
3D structure databases
HSSP	HSSP built from PDB template 2D1X based on UniProtKB Q14247.
ProteinModelPortal	Q2KJB5.
SMR	Q2KJB5. Positions 441-505.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000013682.
Proteomic databases
PRIDE	Q2KJB5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	521834.
KEGG	bta:521834.
Organism-specific databases
CTD	115677.
Phylogenomic databases
eggNOG	NOG266189.
HOGENOM	HOG000113830.
HOVERGEN	HBG101730.
InParanoid	Q2KJB5.
OrthoDB	EOG469QV0.
Family and domain databases
InterPro	IPR001060. FCH_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00611. FCH. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50133. FCH. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20873379.

Entry information

Entry name

NOSTN_BOVIN

Accession

Primary (citable) accession number: Q2KJB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program