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Protein

Nostrin

Gene

NOSTRIN

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane.By similarity

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. regulation of nitric-oxide synthase activity Source: InterPro
  3. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Nostrin
Alternative name(s):
Nitric oxide synthase trafficker
eNOS trafficking inducer
Gene namesi
Name:NOSTRIN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cell membrane By similarity. Cytoplasmic vesicle 1 Publication. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505NostrinPRO_0000289088Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei478 – 4781PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ2KJB5.

Expressioni

Tissue specificityi

Present in pulmonary arterial endothelial cells (at protein level).1 Publication

Interactioni

Subunit structurei

Homotrimer. Interacts with DAB2. Interacts with NOS3, DNM2, WASL and CAV1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013682.

Structurei

3D structure databases

ProteinModelPortaliQ2KJB5.
SMRiQ2KJB5. Positions 441-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 260260F-BARPROSITE-ProRule annotationAdd
BLAST
Domaini437 – 49660SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili101 – 12828Sequence AnalysisAdd
BLAST
Coiled coili160 – 22263Sequence AnalysisAdd
BLAST
Coiled coili295 – 33238Sequence AnalysisAdd
BLAST

Domaini

The SH3 domain mediates interaction with NOS3, DNM2 and WASL.By similarity
The F-BAR domain is necessary for membrane targeting.By similarity

Sequence similaritiesi

Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG266189.
HOGENOMiHOG000113830.
HOVERGENiHBG101730.
InParanoidiQ2KJB5.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR011072. HR1_rho-bd.
IPR028535. Nostrin.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR14167:SF22. PTHR14167:SF22. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KJB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDPLTDCSY NKVYKNLKEF SQNGEDFCKQ ITSILQQRAN LEINYAKGLQ
60 70 80 90 100
KLATKLSKTL QSAKKNCLVS AWAWVSEGMK SAGDLHQKLG KAIELEAIKP
110 120 130 140 150
AHQVLSAHEK KRKSLENEVE KTANLVISNW NQQIKAKKKL MVSTKKHEAL
160 170 180 190 200
FHLVESSKQI TTEKEKQKLL NKLKKSTEKL SKEDENYYQK NVASCSTRLK
210 220 230 240 250
WENTLENCFQ SILELEKERI QLLCNNLNQY SQHISVFGQT LTTCHTQIHC
260 270 280 290 300
AISKIDIEKD IQALMEETTV SSTENKSEFL LTDYFEEDPK NAMSKERQTS
310 320 330 340 350
SIKSKLLRLQ KDIEKASRDQ EGLERMLRAY SSHSSFSDSE SKKSTAALMD
360 370 380 390 400
ENSLKLDLLQ ANSYKLSSVL AELEQRPQPN HPCSNSIFKW KEKQTHSSVK
410 420 430 440 450
ISRPVLMKRL ENVVNRASSD GQRIPSPSST ASGVTQLGNG LCKALYPFQA
460 470 480 490 500
RQDDELDLEK GDIVTIHKKK DEGWWFGSLK GKKGHFPAAY VEELPLNAGD

TASQA
Length:505
Mass (Da):57,307
Last modified:March 7, 2006 - v1
Checksum:i3EE984572780623B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105426 mRNA. Translation: AAI05427.1.
RefSeqiNP_001039722.1. NM_001046257.2.
UniGeneiBt.17865.

Genome annotation databases

GeneIDi521834.
KEGGibta:521834.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105426 mRNA. Translation: AAI05427.1.
RefSeqiNP_001039722.1. NM_001046257.2.
UniGeneiBt.17865.

3D structure databases

ProteinModelPortaliQ2KJB5.
SMRiQ2KJB5. Positions 441-505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013682.

Proteomic databases

PRIDEiQ2KJB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi521834.
KEGGibta:521834.

Organism-specific databases

CTDi115677.

Phylogenomic databases

eggNOGiNOG266189.
HOGENOMiHOG000113830.
HOVERGENiHBG101730.
InParanoidiQ2KJB5.

Miscellaneous databases

NextBioi20873379.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR011072. HR1_rho-bd.
IPR028535. Nostrin.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR14167:SF22. PTHR14167:SF22. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF46585. SSF46585. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.
  2. "Aberrant cytoplasmic sequestration of eNOS in endothelial cells after monocrotaline, hypoxia, and senescence: live-cell caveolar and cytoplasmic NO imaging."
    Mukhopadhyay S., Xu F., Sehgal P.B.
    Am. J. Physiol. 292:H1373-H1389(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNOSTN_BOVIN
AccessioniPrimary (citable) accession number: Q2KJB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 7, 2006
Last modified: March 4, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.