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Protein

AP-1 complex subunit mu-1

Gene

AP1M1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-BTA-2132295. MHC class II antigen presentation.
R-BTA-432720. Lysosome Vesicle Biogenesis.
R-BTA-432722. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-1
Alternative name(s):
AP-mu chain family member mu1A
Adaptor protein complex AP-1 subunit mu-1
Adaptor-related protein complex 1 subunit mu-1
Clathrin assembly protein complex 1 mu-1 medium chain 1
Golgi adaptor HA1/AP1 adaptin mu-1 subunit
Mu-adaptin 1
Mu1A-adaptin
Gene namesi
Name:AP1M1By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 423422AP-1 complex subunit mu-1PRO_0000240589Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei152 – 1521PhosphothreonineBy similarity
Modified residuei154 – 1541PhosphothreonineBy similarity
Modified residuei223 – 2231PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ2KJ81.
PRIDEiQ2KJ81.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017724.

Structurei

3D structure databases

ProteinModelPortaliQ2KJ81.
SMRiQ2KJ81. Positions 2-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 421254MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adaptor complexes medium subunit family.Sequence analysis
Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173247.
HOVERGENiHBG050516.
InParanoidiQ2KJ81.
KOiK12393.
OMAiEMMDFGF.
OrthoDBiEOG783MV6.
TreeFamiTF300393.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KJ81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL
60 70 80 90 100
AHGGVRFMWI KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE
110 120 130 140 150
ESIRDNFVII YELLDELMDF GYPQTTDSKI LQEYITQEGH KLETGAPRPP
160 170 180 190 200
ATVTNAVSWR SEGIKYRKNE VFLDVIESVN LLVSANGNVL RSEIVGSIKM
210 220 230 240 250
RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ CVRLSRFEND
260 270 280 290 300
RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMIKAKSQ
310 320 330 340 350
FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSIKSFPG
360 370 380 390 400
GKEYLMRAHF GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK
410 420
SGYQALPWVR YITQNGDYQL RTQ
Length:423
Mass (Da):48,587
Last modified:January 23, 2007 - v3
Checksum:i64EC5E47EA6F8E98
GO

Sequence cautioni

The sequence AAI05478.1 differs from that shown. Reason: Erroneous termination at position 305. Translated as Ser.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105477 mRNA. Translation: AAI05478.1. Sequence problems.
RefSeqiNP_001039349.2. NM_001045884.2.
UniGeneiBt.23352.

Genome annotation databases

EnsembliENSBTAT00000017724; ENSBTAP00000017724; ENSBTAG00000013329.
GeneIDi504310.
KEGGibta:504310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105477 mRNA. Translation: AAI05478.1. Sequence problems.
RefSeqiNP_001039349.2. NM_001045884.2.
UniGeneiBt.23352.

3D structure databases

ProteinModelPortaliQ2KJ81.
SMRiQ2KJ81. Positions 2-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017724.

Proteomic databases

PaxDbiQ2KJ81.
PRIDEiQ2KJ81.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017724; ENSBTAP00000017724; ENSBTAG00000013329.
GeneIDi504310.
KEGGibta:504310.

Organism-specific databases

CTDi8907.

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173247.
HOVERGENiHBG050516.
InParanoidiQ2KJ81.
KOiK12393.
OMAiEMMDFGF.
OrthoDBiEOG783MV6.
TreeFamiTF300393.

Enzyme and pathway databases

ReactomeiR-BTA-2132295. MHC class II antigen presentation.
R-BTA-432720. Lysosome Vesicle Biogenesis.
R-BTA-432722. Golgi Associated Vesicle Biogenesis.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: HerefordImported.
    Tissue: HypothalamusImported.
  2. "AP-1 binding to sorting signals and release from clathrin-coated vesicles is regulated by phosphorylation."
    Ghosh P., Kornfeld S.
    J. Cell Biol. 160:699-708(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.

Entry informationi

Entry nameiAP1M1_BOVIN
AccessioniPrimary (citable) accession number: Q2KJ81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.