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Protein

Ubiquitin carboxyl-terminal hydrolase 21

Gene

USP21

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211NucleophilePROSITE-ProRule annotation
Metal bindingi384 – 3841ZincBy similarity
Metal bindingi387 – 3871ZincBy similarity
Metal bindingi437 – 4371ZincBy similarity
Metal bindingi440 – 4401ZincBy similarity
Active sitei518 – 5181Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. NEDD8-specific protease activity Source: UniProtKB
  4. transcription coactivator activity Source: UniProtKB
  5. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. histone deubiquitination Source: UniProtKB
  2. positive regulation of transcription, DNA-templated Source: UniProtKB
  3. transcription, DNA-templated Source: UniProtKB-KW
  4. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.034.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 21 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 21
Ubiquitin thioesterase 21
Ubiquitin-specific-processing protease 21
Gene namesi
Name:USP21
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Ubiquitin carboxyl-terminal hydrolase 21PRO_0000367508Add
BLAST

Proteomic databases

PRIDEiQ2KJ72.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000029184.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 558347USPAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 15219Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP21 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000231498.
HOVERGENiHBG011164.
InParanoidiQ2KJ72.
KOiK11833.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KJ72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQASEHRLG RTREPPLNIQ PRVGSKLPFA PRARSKERRN PAPGPNPMLR
60 70 80 90 100
PLPPRPGPPE ERLKKLELGR GRTSGPRPSG PLRADHGVPL PGSPPPTVAL
110 120 130 140 150
PLPSRTNLAR SKSVSSGDLR PMGIALGGHR GTGELGAALS RLALRPEPPP
160 170 180 190 200
LRRSTSLRRL GGFPGPPTLF SIRTEPPTPH GSFHVISARP SEPFYSDDKM
210 220 230 240 250
AHHTLLLGSG HVGLRNLGNT CFLNALLQCL SSTRPLRDFC LRRDFRQEVP
260 270 280 290 300
GGGRAQELTE AFADVIGALW HPDSCEAVNP TRFRAVFQKY VPSFSGYSQQ
310 320 330 340 350
DAQEFLKLLM ERLHLEINRR GRRAPPILAS SPAPHPPRLG GALLEEPELS
360 370 380 390 400
DDDRANLMWK RYLEREDSKI VDLFVGQLKS CLKCQACGYR STTFEVFCDL
410 420 430 440 450
SLPIPKKGFA GGKVSLRDCF NLFTKEEELE SENAPVCDRC RQKTRSTKKL
460 470 480 490 500
TVQRFPRILV LHLNRFSASR GSIKKSSVGV DFPLQRLSLG DFASDKAGSP
510 520 530 540 550
VYQLYALCNH SGSVHYGHYT ALCRCQTGWH VYNDSRVSPV SENQVASSEG
560
YVLFYQLMQE PPRCL
Length:565
Mass (Da):62,619
Last modified:March 6, 2006 - v1
Checksum:i6AA35C030406094C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105488 mRNA. Translation: AAI05489.1.
RefSeqiNP_001039841.1. NM_001046376.2.
UniGeneiBt.24990.

Genome annotation databases

GeneIDi534273.
KEGGibta:534273.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105488 mRNA. Translation: AAI05489.1.
RefSeqiNP_001039841.1. NM_001046376.2.
UniGeneiBt.24990.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000029184.

Protein family/group databases

MEROPSiC19.034.

Proteomic databases

PRIDEiQ2KJ72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi534273.
KEGGibta:534273.

Organism-specific databases

CTDi27005.

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000231498.
HOVERGENiHBG011164.
InParanoidiQ2KJ72.
KOiK11833.

Miscellaneous databases

NextBioi20876334.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.

Entry informationi

Entry nameiUBP21_BOVIN
AccessioniPrimary (citable) accession number: Q2KJ72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: March 6, 2006
Last modified: March 31, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.