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Reviewed, UniProtKB/Swiss-Prot Q2KJ63 (KLKB1_BOVIN)

Last modified June 16, 2009. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Plasma kallikrein
    EC=3.4.21.34
Alternative name(s):
    Plasma prekallikrein
    Kininogenin
    Fletcher factor
Cleaved into the following 2 chains:
    1- Recommended name:
            Plasma kallikrein heavy chain
    2- Recommended name:
            Plasma kallikrein light chain
Gene names
Name: KLKB1
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length636 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin By similarity.

Catalytic activity

Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.

Subunit structure

The zymogen is activated by factor XIIa, which cleaves the molecule into a light chain, which contains the active site, and a heavy chain, which associates with HMW kininogen. These chains are linked by one or more disulfide bonds By similarity.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Contains 4 apple domains.

Contains 1 peptidase S1 domain.

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Ontologies

Keywords
   Biological processBlood coagulation
Fibrinolysis
Inflammatory response
   Cellular componentSecreted
   DomainRepeat
Signal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
Gene Ontology (GO)
   Biological processblood coagulation

Inferred from electronic annotation. Source: UniProtKB-KW

fibrinolysis

Inferred from electronic annotation. Source: UniProtKB-KW

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 By similarity
Chain20 – 391372Plasma kallikrein heavy chain
PRO_0000285880
Chain392 – 636245Plasma kallikrein light chain
PRO_0000285881

Regions

Domain21 – 10484Apple 1
Domain111 – 19484Apple 2
Domain201 – 28484Apple 3
Domain294 – 37784Apple 4
Domain392 – 627236Peptidase S1

Sites

Active site4351Charge relay system By similarity
Active site4841Charge relay system By similarity
Active site5791Charge relay system By similarity

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation1271N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential
Glycosylation3971N-linked (GlcNAc...) Potential
Glycosylation4541N-linked (GlcNAc...) Potential
Glycosylation4951N-linked (GlcNAc...) Potential
Disulfide bond21 ↔ 104 By similarity
Disulfide bond47 ↔ 77 By similarity
Disulfide bond51 ↔ 57 By similarity
Disulfide bond111 ↔ 194 By similarity
Disulfide bond137 ↔ 166 By similarity
Disulfide bond141 ↔ 147 By similarity
Disulfide bond201 ↔ 284 By similarity
Disulfide bond227 ↔ 256 By similarity
Disulfide bond231 ↔ 237 By similarity
Disulfide bond294 ↔ 377 By similarity
Disulfide bond320 ↔ 349 By similarity
Disulfide bond324 ↔ 330 By similarity
Disulfide bond420 ↔ 436 By similarity
Disulfide bond518 ↔ 585 By similarity
Disulfide bond549 ↔ 564 By similarity
Disulfide bond575 ↔ 603 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q2KJ63-1 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 75DE0562291F03F2

FASTA63670,994
        10         20         30         40         50         60 
MIALRQAAYF ICLFATVSCG CLTQLYHNIF FRGGDVSAMY TPDAQYCQLM CTFHPRCLLF 

        70         80         90        100        110        120 
SFLPENSTSD ADKRFGCFLK DSVTGTLPRV SRTGAISGHS LKRCGHQISA CHRSIYKGID 

       130        140        150        160        170        180 
MRGVNFNASK VRSAKECQER CTNNIHCQFF TYATKTFFSA EYRNTCLLKR SPQGTPTRIK 

       190        200        210        220        230        240 
VLSDVESGFS LKACGNSKIG CRVDIFQHSA FSDVDVAGII APDAFVCRTI CTYHPSCLFF 

       250        260        270        280        290        300 
TFYTNAWKTD SQRNVCFLKT SQSGSPSSPT PQENAISGYS LLTCKQTLPG TEPCHSKIYP 

       310        320        330        340        350        360 
QVAFEGEELH VTFVKGVDGC QETCTKMIRC QFFTYSLFPE DCRGEKCKCS LRLSLDGSPT 

       370        380        390        400        410        420 
NITYGTQASS GYSLRLCKRG DSRVCTTKRT RIVGGTNASW GEWPWQVSLQ VKQRAQSHLC 

       430        440        450        460        470        480 
GGSIIGRQWV LTAAHCFDGL LLSNIWRIYG GILNLSEITT ETSFSQIKEI IVHPNYKISE 

       490        500        510        520        530        540 
GSHDIALIKL EAPLNFTDLQ KAICLPSKDD TKPVYTDCWI TGWGFTEEKG KIQNTLQKAN 

       550        560        570        580        590        600 
IPLISNEECQ KSYRDYKITK QMICAGYKEG GKDACKGDSG GPLVCQHEET WHLVGITSWG 

       610        620        630 
EGCARREQPG VYTKVAEYVD WILEKTQDSH GQPLRK

« Hide

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References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal liver.

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Cross-references

Sequence databases

BC105498 mRNA. Translation: AAI05499.1.
IPIIPI00699386.
RefSeqNP_001039817.1.
UniGeneBt.55378

3D structure databases

ModBaseSearch...

Protein family/group databases

MEROPSS01.212.

Genome annotation databases

EnsemblENSBTAG00000009501. Bos taurus. [Contig view]
GeneID533547.
KEGGbta:533547.

Phylogenomic databases

HOVERGENQ2KJ63.

Enzyme and pathway databases

BRENDA3.4.21.34. 251.

Family and domain databases

InterProIPR000177. Apple.
IPR003014. PAN-1_domain.
IPR003609. Pan_app.
IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]
PfamPF00024. PAN_1. 4 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00005. APPLEDOMAIN.
PR00722. CHYMOTRYPSIN.
SMARTSM00223. APPLE. 4 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
PROSITEPS00495. APPLE. 4 hits.
PS50948. PAN. 4 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameKLKB1_BOVIN
AccessionPrimary (citable) accession number: Q2KJ63
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: March 7, 2006
Last modified: June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents