Reviewed, UniProtKB/Swiss-Prot Q2KJ61 (ELP3_BOVIN)

Names and origin

Protein names

Recommended name:
    Elongator complex protein 3
    EC=2.3.1.48

Gene names

Name:

ELP3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. May also have a methyltransferase activity By similarity.
Catalytic activity	Acetyl-CoA + histone = CoA + acetylhistone.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Component of the RNA polymerase II elongator complex (Elongator), which consists of IKBKAP/ELP1, STIP1/ELP2, ELP3, ELP4, and two yet unidentified proteins. IKBKAP/ELP1, STIP1/ELP2 and ELP3 form the Elongator core complex. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with IKBKAP/ELP1 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the ELP3 family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB transcription elongation factor complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	histone acetyltransferase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorylase kinase regulator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	Elongator complex protein 3		PRO_0000283985
Regions
	Domain	396 – 547	152	N-acetyltransferase
Sites
	Metal binding	99	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	109	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	112	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Amino acid modifications
	Modified residue	202	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KJ61-1 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: 1B2F2B06FA019F81	FASTA	547	62,331
	10 20 30 40 50 60 MRQKRKGDLS PAQLMMLTIG DVIKQLIEAH EQGKDIDLNK VKTRTAAKYG LSAQPRLVDI 70 80 90 100 110 120 IAAVPPQYRK VLVPKLKAKP IRTASGIAVV AVMCKPHRCP HISFTGNICV YCPGGPDSDF 130 140 150 160 170 180 EYSTQSYTGY EPTSMRAIRA RYDPYLQTRH RIEQLKQLGH SVDKVEFIVM GGTFMALPEE 190 200 210 220 230 240 YRDYFIRNLH DALSGHTSNN IYEAVKYSER SLTKCIGITI ETRPDYCMKR HLSDMLTYGC 250 260 270 280 290 300 TRLEIGVQSV YEDVARDTNR GHTVKAVCES FHLAKDSGFK VVAHMMPDLP NVGLERDIEQ 310 320 330 340 350 360 FTEFFENPAF RPDGLKLYPT LVIRGTGLYE LWKSGRYKSY SPSDLIELVA RILALVPPWT 370 380 390 400 410 420 RVYRVQRDIP MPLVSSGVEH GNLRELAFAR MKDLGIQCRD VRTREVGIQE IHHKVRPYQV 430 440 450 460 470 480 ELVRRDYVAN GGWETFLSYE DPDQDILIGL LRLRKCSEET FRFELVGGVS IVRELHVYGS 490 500 510 520 530 540 VVPVSSRDPT KFQHQGFGML LMEEAERIAR EEHGSGKIAV ISGVGTRNYY RKIGYRLQGP YMVKTLE

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC105500 mRNA. Translation: AAI05501.2.
IPI	IPI00691160.
RefSeq	NP_001124234.1.
UniGene	Bt.40884
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000002730. Bos taurus. [Contig view]
GeneID	784720.
KEGG	bta:784720.
Phylogenomic databases
HOVERGEN	Q2KJ61.
OMA	Q2KJ61. GIQEVHH.
Enzyme and pathway databases
BRENDA	2.3.1.48. 251.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR000182. GCN5-rel_AcTrfase. IPR005910. Hist_AcTrfase_ELP3. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005669. Hist_AcTrfase_ELP3. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01211. ELP3. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELP3_BOVIN

Accession

Primary (citable) accession number: Q2KJ61

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	April 17, 2007
Last modified:	June 16, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)