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Protein

Inositol monophosphatase 3

Gene

IMPAD1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation.By similarity

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

Strongly inhibited by lithium.By similarity

Pathwayi: myo-inositol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Inositol-3-phosphate synthase 1 (ISYNA1)
  2. Inositol monophosphatase 3 (IMPAD1), Inositol monophosphatase 1 (IMPA1)
This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136Magnesium 1By similarity1
Binding sitei136SubstrateBy similarity1
Metal bindingi177Magnesium 1By similarity1
Metal bindingi177Magnesium 2By similarity1
Metal bindingi179Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi180Magnesium 2By similarity1
Metal bindingi303Magnesium 2By similarity1
Binding sitei303SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol monophosphatase 3 (EC:3.1.3.25, EC:3.1.3.7)
Short name:
IMP 3
Short name:
IMPase 3
Alternative name(s):
Inositol monophosphatase domain-containing protein 1
Inositol-1(or 4)-monophosphatase 3
Myo-inositol monophosphatase A3
Gene namesi
Name:IMPAD1
Synonyms:IMPA3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 362LumenalSequence analysisAdd BLAST329

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002890401 – 362Inositol monophosphatase 3Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi262N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Contains N-linked glycan resistant to endoglycosydase H.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ2KJ53.
PRIDEiQ2KJ53.

Expressioni

Gene expression databases

BgeeiENSBTAG00000015637.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020766.

Structurei

3D structure databases

ProteinModelPortaliQ2KJ53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 182Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3853. Eukaryota.
COG1218. LUCA.
GeneTreeiENSGT00530000063462.
HOGENOMiHOG000290671.
HOVERGENiHBG062091.
InParanoidiQ2KJ53.
KOiK15759.
OMAiGQTAWAW.
OrthoDBiEOG091G0RCH.
TreeFamiTF314300.

Family and domain databases

InterProiIPR000760. Inositol_monophosphatase-like.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PROSITEiPS00630. IMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KJ53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPMGIRLSP LGVAVFCLLG LGVLYHLYSG FLAGRFSLFG LGGEPGGGAA
60 70 80 90 100
GPAGPAASAD GGTVDLREML AVSVLAAVRG GEEVRRVRES NVLHEKSKGK
110 120 130 140 150
TREGADDKMT SGDVLSNRKM FYLLKTAFPS VQINTEEHVD ASDQEVILWD
160 170 180 190 200
RKIPEDILKE IATPQEVPAE SVTVWIDPLD ATQEYTEDLR KYVTTMVCVA
210 220 230 240 250
VNGKPVLGVI HKPFSEYTAW AMVDGGSNVK ARTSYNEKTP RIVVSRSHSG
260 270 280 290 300
MVKQVALQTF GNQTTIIPAG GAGYKVLALL DVPDKSQEKA DLYIHVTYIK
310 320 330 340 350
KWDICAGNAI LKALGGHMTT LSGEEISYTG SDGIEGGLLA SIRMNHQALV
360
RKLPDLEKTG HK
Length:362
Mass (Da):38,924
Last modified:March 7, 2006 - v1
Checksum:i2F8EBBE45DB03520
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105514 mRNA. Translation: AAI05515.1.
RefSeqiNP_001039800.1. NM_001046335.1.
UniGeneiBt.12649.

Genome annotation databases

EnsembliENSBTAT00000020766; ENSBTAP00000020766; ENSBTAG00000015637.
GeneIDi532797.
KEGGibta:532797.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105514 mRNA. Translation: AAI05515.1.
RefSeqiNP_001039800.1. NM_001046335.1.
UniGeneiBt.12649.

3D structure databases

ProteinModelPortaliQ2KJ53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020766.

Proteomic databases

PaxDbiQ2KJ53.
PRIDEiQ2KJ53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000020766; ENSBTAP00000020766; ENSBTAG00000015637.
GeneIDi532797.
KEGGibta:532797.

Organism-specific databases

CTDi54928.

Phylogenomic databases

eggNOGiKOG3853. Eukaryota.
COG1218. LUCA.
GeneTreeiENSGT00530000063462.
HOGENOMiHOG000290671.
HOVERGENiHBG062091.
InParanoidiQ2KJ53.
KOiK15759.
OMAiGQTAWAW.
OrthoDBiEOG091G0RCH.
TreeFamiTF314300.

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.

Gene expression databases

BgeeiENSBTAG00000015637.

Family and domain databases

InterProiIPR000760. Inositol_monophosphatase-like.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PROSITEiPS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMPA3_BOVIN
AccessioniPrimary (citable) accession number: Q2KJ53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 7, 2006
Last modified: November 30, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.