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Q2KJ16 (PHKG2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

Short name=PHK-gamma-LT
Short name=PHK-gamma-T
EC=2.7.11.19
Alternative name(s):
Phosphorylase kinase subunit gamma-2
Gene names
Name:PHKG2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM By similarity.

Catalytic activity

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.

Subunit structure

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
PRO_0000244591

Regions

Domain24 – 291268Protein kinase
Nucleotide binding30 – 389ATP By similarity
Region306 – 33025Calmodulin-binding (domain-N) By similarity
Region346 – 37025Calmodulin-binding (domain-C) By similarity

Sites

Active site1531Proton acceptor By similarity
Binding site531ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2KJ16 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: E0B8F8494DF8A58E

FASTA40646,542
        10         20         30         40         50         60 
MTLDVGPEDE LPDWAAAKEF YQKYDPKDVI GRGVSSVVRR CVHRATGQEF AVKIMEVTAE 

        70         80         90        100        110        120 
RLSPEQLEEV REATRRETHI LRQVAGHPHI ITLIDSYESS SFMFLVFDLM RKGELFDYLT 

       130        140        150        160        170        180 
EKVALSEKET RSIMRSLLEA VSFLHNNNIV HRDLKPENIL LDDNMQIRLS DFGFSCHLEP 

       190        200        210        220        230        240 
GEKLRELCGT PGYLAPEILK CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL 

       250        260        270        280        290        300 
MLRMIMEGQY QFSSPEWDDR SDTVKDLISR LLQVDPVERL TAEQALQHPF FERCEGSQAW 

       310        320        330        340        350        360 
NLTPRQRFRV AVWTVLAAGR VALSAHRIRP LTKSALLRDP YALRPVRRLI DNCAFRLYGH 

       370        380        390        400 
WVKKGEQQNR AALFQHRPPG PFPMMGPEEE GDSATIAEDE AMLVLG 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT030706 mRNA. Translation: ABS45022.1.
BC105568 mRNA. Translation: AAI05569.1.
RefSeqNP_001039593.1. NM_001046128.1.
UniGeneBt.11190.

3D structure databases

ProteinModelPortalQ2KJ16.
SMRQ2KJ16. Positions 20-293.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ2KJ16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000004433; ENSBTAP00000004433; ENSBTAG00000003417.
GeneID512670.
KEGGbta:512670.

Organism-specific databases

CTD5261.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117716.
HOGENOMHOG000233016.
HOVERGENHBG106193.
InParanoidQ2KJ16.
KOK00871.
OMAHRPPGPF.
OrthoDBEOG7JMGDM.
TreeFamTF320349.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01049. PHOSPHBKNASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20870499.

Entry information

Entry namePHKG2_BOVIN
AccessionPrimary (citable) accession number: Q2KJ16
Secondary accession number(s): A7E3T5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 7, 2006
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families