Q2KJ16 (PHKG2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
Short name=PHK-gamma-LT
Short name=PHK-gamma-T
EC=2.7.11.19

Alternative name(s):
Phosphorylase kinase subunit gamma-2

Gene names

Name:

PHKG2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM By similarity.
Catalytic activity	2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
Subunit structure	Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	phosphorylase kinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorylase kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Phosphorylase b kinase gamma catalytic chain, liver/testis isoform		PRO_0000244591
Regions
	Domain	24 – 291	268	Protein kinase
	Nucleotide binding	30 – 38	9	ATP By similarity
	Region	306 – 330	25	Calmodulin-binding (domain-N) By similarity
	Region	346 – 370	25	Calmodulin-binding (domain-C) By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	53	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KJ16 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: E0B8F8494DF8A58E	FASTA	406	46,542
	10 20 30 40 50 60 MTLDVGPEDE LPDWAAAKEF YQKYDPKDVI GRGVSSVVRR CVHRATGQEF AVKIMEVTAE 70 80 90 100 110 120 RLSPEQLEEV REATRRETHI LRQVAGHPHI ITLIDSYESS SFMFLVFDLM RKGELFDYLT 130 140 150 160 170 180 EKVALSEKET RSIMRSLLEA VSFLHNNNIV HRDLKPENIL LDDNMQIRLS DFGFSCHLEP 190 200 210 220 230 240 GEKLRELCGT PGYLAPEILK CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL 250 260 270 280 290 300 MLRMIMEGQY QFSSPEWDDR SDTVKDLISR LLQVDPVERL TAEQALQHPF FERCEGSQAW 310 320 330 340 350 360 NLTPRQRFRV AVWTVLAAGR VALSAHRIRP LTKSALLRDP YALRPVRRLI DNCAFRLYGH 370 380 390 400 WVKKGEQQNR AALFQHRPPG PFPMMGPEEE GDSATIAEDE AMLVLG

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT030706 mRNA. Translation: ABS45022.1. BC105568 mRNA. Translation: AAI05569.1.
IPI	IPI00688904.
RefSeq	NP_001039593.1. NM_001046128.1.
UniGene	Bt.11190.
3D structure databases
ProteinModelPortal	Q2KJ16.
SMR	Q2KJ16. Positions 20-293.
ModBase	Search...
Proteomic databases
PRIDE	Q2KJ16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000004433; ENSBTAP00000004433; ENSBTAG00000003417.
GeneID	512670.
KEGG	bta:512670.
Organism-specific databases
CTD	5261.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00680000100008.
HOGENOM	HOG000233016.
HOVERGEN	HBG106193.
InParanoid	Q2KJ16.
KO	K00871.
OMA	HQPPGPF.
OrthoDB	EOG4CNQR5.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR002291. Phosph_kin_gamma. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01049. PHOSPHBKNASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20870499.

Entry information

Entry name

PHKG2_BOVIN

Accession

Primary (citable) accession number: Q2KJ16
Secondary accession number(s): A7E3T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 7, 2006
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program