Q2KJ09 (UBP16_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 57.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin carboxyl-terminal hydrolase 16 EC=3.4.19.12 Alternative name(s): Deubiquitinating enzyme 16 Ubiquitin thioesterase 16 Ubiquitin-specific-processing protease 16 | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 826 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | Nucleus By similarity. |
| Domain | The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin By similarity. |
| Post-translational modification | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active By similarity. |
| Sequence similarities | Belongs to the peptidase C19 family. USP16 subfamily. Contains 1 UBP-type zinc finger. |
| Sequence caution | The sequence AAI12387.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q2KJ09-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q2KJ09-2) The sequence of this isoform differs from the canonical sequence as follows: 149-149: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 826 | 826 | Ubiquitin carboxyl-terminal hydrolase 16 | PRO_0000367504 | |||||
Regions | |||||||||
| Zinc finger | 46 – 124 | 79 | UBP-type | ||||||
Sites | |||||||||
| Active site | 204 | 1 | Nucleophile By similarity | ||||||
| Active site | 760 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 24 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 26 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 48 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 51 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 73 | 1 | Zinc 3 By similarity | ||||||
| Metal binding | 76 | 1 | Zinc 3 By similarity | ||||||
| Metal binding | 81 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 87 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 89 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 93 | 1 | Zinc 3 By similarity | ||||||
| Metal binding | 102 | 1 | Zinc 3 By similarity | ||||||
| Metal binding | 115 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 118 | 1 | Zinc 1 By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 414 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 149 | 1 | Missing in isoform 2. | VSP_036725 | |||||
Sequences
| ||||||||||||||||||||||||
References
| [1] | Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-659 (ISOFORM 2). Tissue: Prostate. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CH473989 Genomic DNA. Translation: EDM10641.1. BC112386 mRNA. Translation: AAI12387.1. Sequence problems. |
| IPI | IPI00190797. IPI00923707. |
| RefSeq | NP_001093971.1. NM_001100501.1. |
| UniGene | Rn.55800. |
3D structure databases | |
| HSSP | HSSP built from PDB template 2GFO based on UniProtKB P40818. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | C19.050. |
Proteomic databases | |
| PaxDb | Q2KJ09. |
| PRIDE | Q2KJ09. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOT00000002173; ENSRNOP00000002173; ENSRNOG00000001598. |
| GeneID | 288306. |
| KEGG | rno:288306. |
| UCSC | RGD:1307192. rat. |
Organism-specific databases | |
| CTD | 10600. |
| RGD | 1307192. Usp16. |
Phylogenomic databases | |
| eggNOG | COG5207. |
| GeneTree | ENSGT00690000102047. |
| HOGENOM | HOG000154755. |
| HOVERGEN | HBG062704. |
| InParanoid | Q2KJ09. |
| KO | K11844. |
| OMA | CKNVAEE. |
| OrthoDB | EOG495ZRD. |
Gene expression databases | |
| Genevestigator | Q2KJ09. |
Family and domain databases | |
| Gene3D | 3.30.40.10. 1 hit. |
| InterPro | IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view] |
| Pfam | PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view] |
| PROSITE | PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 627930. |
Entry information
| Entry name | UBP16_RAT | ||||||||
| Accession | Primary (citable) accession number: Q2KJ09 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |