Reviewed, UniProtKB/Swiss-Prot Q2KIQ5 (T23O_BOVIN)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:

TDO2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Domain	Coiled coil
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: InterPro
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Tryptophan 2,3-dioxygenase		PRO_0000247474
Regions
	Region	42 – 46	5	Substrate binding By similarity
	Region	72 – 76	5	Substrate binding By similarity
	Coiled coil	231 – 271	41	Potential
Sites
	Metal binding	328	1	Iron (heme axial ligand) By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	151	1	Heme By similarity
	Binding site	342	1	Substrate By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine By similarity
	Modified residue	155	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KIQ5-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: D68CD8A6BC59A882	FASTA	406	47,708
	10 20 30 40 50 60 MSGCPFLGKS FGYAFKPLSA QGSEEDKSQA GVNRASKGGL IYGNYLQLEK VLNAQELQSE 70 80 90 100 110 120 MKGNKIHDEH LFIITHQAYE LWFKQILWEL DSVREIFQNG HVRDERNMLK VITRMHRVVV 130 140 150 160 170 180 ILKLLVQQFS VLETMTALDF NDFREYLSPA SGFQSLQFRL LENKIGVLQS LRVPYNRRHY 190 200 210 220 230 240 RDNFRGKDNE LLLKSEQERT LLQLVEAWLE RTPGLEPHGF NFWGKLEKNI VKGLEEEFTK 250 260 270 280 290 300 IQAKEESEEK EEQMAEFQKQ KEVLLSLFDE KRHEHLLSKG ERRLSYKALQ GALMIYFYRE 310 320 330 340 350 360 EPRFQVPFQL LTFLMDVDSL MTKWRYNHVC LVHRMLGSKA GTGGSSGYQY LRSTVSDRYK 370 380 390 400 VFVDLFNLST YLVPRHWIPK MNPVIHKFLY TAEYCDSSYF SSDESD

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC112550 mRNA. Translation: AAI12551.1.
IPI	IPI00714687.
RefSeq	NP_001039778.1.
UniGene	Bt.55173
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000011062. Bos taurus. [Contig view]
GeneID	530397.
KEGG	bta:530397.
Phylogenomic databases
HOVERGEN	Q2KIQ5.
OMA	Q2KIQ5. HYRDNFR.
Enzyme and pathway databases
BRENDA	1.13.11.11. 251.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_BOVIN

Accession

Primary (citable) accession number: Q2KIQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)