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Protein

Porphobilinogen deaminase

Gene

HMBS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.By similarity

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

Cofactori

dipyrromethaneNote: Binds 1 dipyrromethane group covalently.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (ALAD)
  2. Porphobilinogen deaminase (HMBS)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (UROD)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

ReactomeiR-BTA-189451. Heme biosynthesis.
UniPathwayiUPA00251; UER00319.

Names & Taxonomyi

Protein namesi
Recommended name:
Porphobilinogen deaminase (EC:2.5.1.61)
Alternative name(s):
Hydroxymethylbilane synthase
Short name:
HMBS
Gene namesi
Name:HMBS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 15

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 361360Porphobilinogen deaminasePRO_0000244405Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei74 – 741N6-acetyllysineBy similarity
Modified residuei261 – 2611S-(dipyrrolylmethanemethyl)cysteineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ2KIN5.
PRIDEiQ2KIN5.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007052.

Structurei

3D structure databases

ProteinModelPortaliQ2KIN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.Curated

Phylogenomic databases

eggNOGiKOG2892. Eukaryota.
COG0181. LUCA.
GeneTreeiENSGT00390000009083.
HOGENOMiHOG000228587.
HOVERGENiHBG000967.
InParanoidiQ2KIN5.
KOiK01749.
OMAiGIECRTD.
OrthoDBiEOG7W41C4.
TreeFamiTF105389.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam.
InterProiIPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 2 hits.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 2 hits.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KIN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGNGNAAAI AEEDTPKMRV IRVGTRKSQL ARIQTDSVVA TLKALYPGLQ
60 70 80 90 100
FEIIAMSTTG DKILDTALSK IGEKSLFTKE LEHALERNEV DLVVHSLKDL
110 120 130 140 150
PTVLPPGFTI GAVCKRESPY DAVVFHPKFV GKTLETLPEK SVVGTSSLRR
160 170 180 190 200
AAQLQRKFPH LEFKSIRGNL NTRLRKLDEL QEFSAIILAT AGLQRMGWQN
210 220 230 240 250
RVGQILHPEE CMYAVGQGAL GVEVRAKDQD ILDLVGVLHD PETLLRCIAE
260 270 280 290 300
RSFLRHLEGG CSVPVAVHTA IKDGQLYLTG GVWSLNGAET MQDTMQTTIH
310 320 330 340 350
VPVQHEDGPE DDPQLVGITA RNIPRQPQLA AENLGISLAT LLLNKGAKNI
360
LDVARQLNEA H
Length:361
Mass (Da):39,515
Last modified:March 7, 2006 - v1
Checksum:i15596E68C92E80C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112573 mRNA. Translation: AAI12574.1.
RefSeqiNP_001039672.1. NM_001046207.1.
UniGeneiBt.21218.

Genome annotation databases

EnsembliENSBTAT00000007052; ENSBTAP00000007052; ENSBTAG00000005364.
GeneIDi515614.
KEGGibta:515614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112573 mRNA. Translation: AAI12574.1.
RefSeqiNP_001039672.1. NM_001046207.1.
UniGeneiBt.21218.

3D structure databases

ProteinModelPortaliQ2KIN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007052.

Proteomic databases

PaxDbiQ2KIN5.
PRIDEiQ2KIN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007052; ENSBTAP00000007052; ENSBTAG00000005364.
GeneIDi515614.
KEGGibta:515614.

Organism-specific databases

CTDi3145.

Phylogenomic databases

eggNOGiKOG2892. Eukaryota.
COG0181. LUCA.
GeneTreeiENSGT00390000009083.
HOGENOMiHOG000228587.
HOVERGENiHBG000967.
InParanoidiQ2KIN5.
KOiK01749.
OMAiGIECRTD.
OrthoDBiEOG7W41C4.
TreeFamiTF105389.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00319.
ReactomeiR-BTA-189451. Heme biosynthesis.

Miscellaneous databases

NextBioi20871921.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam.
InterProiIPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 2 hits.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 2 hits.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Testis.

Entry informationi

Entry nameiHEM3_BOVIN
AccessioniPrimary (citable) accession number: Q2KIN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 7, 2006
Last modified: December 9, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.