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Protein

Carboxypeptidase B2

Gene

CPB2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

Catalytic activityi

Release of C-terminal Arg and Lys from a polypeptide.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Enzyme regulationi

TAFI/CPB2 is unique among carboxypeptidases in that it spontaneously inactivates with a short half-life, a property that is crucial for its role in controlling blood clot lysis. The zymogen is stabilized by interactions with the activation peptide. Release of the activation peptide increases a dynamic flap mobility and in time this leads to conformational changes that disrupt the catalytic site and expose a cryptic thrombin-cleavage site present at Arg-324 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi181 – 1811Zinc; catalytic1 Publication
Metal bindingi184 – 1841Zinc; catalytic1 Publication
Binding sitei239 – 2391SubstrateBy similarity
Metal bindingi310 – 3101Zinc; catalytic1 Publication
Binding sitei363 – 3631SubstrateBy similarity
Active sitei385 – 3851Proton donor/acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Blood coagulation, Fibrinolysis, Hemostasis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.20. 908.

Protein family/group databases

MEROPSiM14.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase B2 (EC:3.4.17.20)
Alternative name(s):
Carboxypeptidase U
Short name:
CPU
Plasma carboxypeptidase B
Short name:
pCPB
Thrombin-activable fibrinolysis inhibitor
Short name:
TAFI
Gene namesi
Name:CPB2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 12

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Propeptidei23 – 11492Activation peptidePRO_0000282869Add
BLAST
Chaini115 – 423309Carboxypeptidase B2PRO_0000282870Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)1 Publication
Glycosylationi73 – 731N-linked (GlcNAc...)1 Publication
Glycosylationi85 – 851N-linked (GlcNAc...)1 Publication
Glycosylationi108 – 1081N-linked (GlcNAc...)1 Publication
Disulfide bondi178 ↔ 1911 Publication
Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence analysis
Disulfide bondi250 ↔ 2741 Publication
Disulfide bondi265 ↔ 2791 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei324 – 3252Cleavage; by thrombinBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ2KIG3.
PRIDEiQ2KIG3.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009300.

Structurei

Secondary structure

1
423
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 316Combined sources
Helixi36 – 4813Combined sources
Beta strandi49 – 5810Combined sources
Helixi59 – 613Combined sources
Beta strandi68 – 736Combined sources
Helixi74 – 763Combined sources
Helixi77 – 8610Combined sources
Beta strandi91 – 966Combined sources
Helixi98 – 1058Combined sources
Turni106 – 1083Combined sources
Beta strandi109 – 1124Combined sources
Helixi119 – 1213Combined sources
Helixi126 – 13914Combined sources
Turni141 – 1433Combined sources
Beta strandi144 – 1518Combined sources
Beta strandi157 – 1637Combined sources
Beta strandi173 – 1775Combined sources
Helixi186 – 20015Combined sources
Turni202 – 2043Combined sources
Helixi206 – 2149Combined sources
Beta strandi216 – 2216Combined sources
Helixi225 – 2339Combined sources
Helixi255 – 2573Combined sources
Turni262 – 2654Combined sources
Beta strandi269 – 2713Combined sources
Helixi287 – 29812Combined sources
Turni299 – 3024Combined sources
Beta strandi303 – 3108Combined sources
Beta strandi312 – 3198Combined sources
Beta strandi321 – 3255Combined sources
Helixi330 – 34718Combined sources
Beta strandi354 – 3574Combined sources
Helixi358 – 3614Combined sources
Helixi369 – 3768Combined sources
Beta strandi379 – 3857Combined sources
Beta strandi389 – 3924Combined sources
Helixi398 – 4003Combined sources
Helixi401 – 42222Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D4UX-ray1.70A115-423[»]
3DGVX-ray2.50A/B/C23-423[»]
3OSLX-ray6.00A/C23-423[»]
ProteinModelPortaliQ2KIG3.
SMRiQ2KIG3. Positions 23-423.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2KIG3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1844Substrate bindingBy similarity
Regioni256 – 2572Substrate bindingBy similarity
Regioni311 – 3122Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiQ2KIG3.
KOiK01300.
OMAiLRRNINH.
OrthoDBiEOG7RZ5Q9.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KIG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLYSLGVLV ATVLFCGEHA FAFQRGQVLS ALPRTSRQVQ ILQNVTTTYK
60 70 80 90 100
IVLWQPVAAE YIVKGYEVHF FVNASDVSNV KAHLNASRIP FRVLVENVED
110 120 130 140 150
LIRQQTSNDT ISPRASSSYY EQYHSLNEIY SWIEVMTERY PDMVEKIHIG
160 170 180 190 200
SSYEKYPLYV LKVSKKEQRA KNAMWIDCGI HAREWISPAF CLWFVGSVTY
210 220 230 240 250
YYGKEKMHTN LLKHMDFYIM PVVNVDGYDY TWKKDRMWRK NRSLHEKNAC
260 270 280 290 300
VGTDLNRNFA SKHWCGEGAS SSSCSEIYCG TYPESEPEVK AVADFLRRNI
310 320 330 340 350
KHIKAYISMH SYSQKIVFPY SYSRSRSKDH EELSLVAREA VFAMENIHRN
360 370 380 390 400
IRYTHGSGSE SLYLAPGGSD DWIYDLGIKY SFTFELRDKG KYGFLLPESY
410 420
IRPTCSEALV AVAKIASHVV KNV
Length:423
Mass (Da):48,822
Last modified:March 7, 2006 - v1
Checksum:i361E7FE3F885080E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112649 mRNA. Translation: AAI12650.1.
RefSeqiNP_001039462.1. NM_001045997.2.
UniGeneiBt.93764.

Genome annotation databases

EnsembliENSBTAT00000009300; ENSBTAP00000009300; ENSBTAG00000007073.
GeneIDi508222.
KEGGibta:508222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112649 mRNA. Translation: AAI12650.1.
RefSeqiNP_001039462.1. NM_001045997.2.
UniGeneiBt.93764.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D4UX-ray1.70A115-423[»]
3DGVX-ray2.50A/B/C23-423[»]
3OSLX-ray6.00A/C23-423[»]
ProteinModelPortaliQ2KIG3.
SMRiQ2KIG3. Positions 23-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009300.

Protein family/group databases

MEROPSiM14.009.

Proteomic databases

PaxDbiQ2KIG3.
PRIDEiQ2KIG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000009300; ENSBTAP00000009300; ENSBTAG00000007073.
GeneIDi508222.
KEGGibta:508222.

Organism-specific databases

CTDi1361.

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiQ2KIG3.
KOiK01300.
OMAiLRRNINH.
OrthoDBiEOG7RZ5Q9.
TreeFamiTF317197.

Enzyme and pathway databases

BRENDAi3.4.17.20. 908.

Miscellaneous databases

EvolutionaryTraceiQ2KIG3.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Testis.
  2. "The crystal structure of thrombin-activable fibrinolysis inhibitor (TAFI) provides the structural basis for its intrinsic activity and the short half-life of TAFIa."
    Anand K., Pallares I., Valnickova Z., Christensen T., Vendrell J., Wendt K.U., Schreuder H.A., Enghild J.J., Aviles F.X.
    J. Biol. Chem. 283:29416-29423(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 23-423 IN COMPLEX WITH ZINC IONS, GLYCOSYLATION AT ASN-44; ASN-73; ASN-85 AND ASN-108, ACTIVE SITE, ZINC-BINDING SITES, DISULFIDE BONDS, COFACTOR.

Entry informationi

Entry nameiCBPB2_BOVIN
AccessioniPrimary (citable) accession number: Q2KIG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 7, 2006
Last modified: June 8, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.