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Protein

Carboxypeptidase B2

Gene

CPB2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

Catalytic activityi

Release of C-terminal Arg and Lys from a polypeptide.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Enzyme regulationi

TAFI/CPB2 is unique among carboxypeptidases in that it spontaneously inactivates with a short half-life, a property that is crucial for its role in controlling blood clot lysis. The zymogen is stabilized by interactions with the activation peptide. Release of the activation peptide increases a dynamic flap mobility and in time this leads to conformational changes that disrupt the catalytic site and expose a cryptic thrombin-cleavage site present at Arg-324 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi181Zinc; catalytic1 Publication1
Metal bindingi184Zinc; catalytic1 Publication1
Binding sitei239SubstrateBy similarity1
Metal bindingi310Zinc; catalytic1 Publication1
Binding sitei363SubstrateBy similarity1
Active sitei385Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Blood coagulation, Fibrinolysis, Hemostasis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.20. 908.

Protein family/group databases

MEROPSiM14.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase B2 (EC:3.4.17.20)
Alternative name(s):
Carboxypeptidase U
Short name:
CPU
Plasma carboxypeptidase B
Short name:
pCPB
Thrombin-activable fibrinolysis inhibitor
Short name:
TAFI
Gene namesi
Name:CPB2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 12

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000028286923 – 114Activation peptideAdd BLAST92
ChainiPRO_0000282870115 – 423Carboxypeptidase B2Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...)1 Publication1
Glycosylationi73N-linked (GlcNAc...)1 Publication1
Glycosylationi85N-linked (GlcNAc...)1 Publication1
Glycosylationi108N-linked (GlcNAc...)1 Publication1
Disulfide bondi178 ↔ 1911 Publication
Glycosylationi241N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi250 ↔ 2741 Publication
Disulfide bondi265 ↔ 2791 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei324 – 325Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ2KIG3.
PRIDEiQ2KIG3.

Expressioni

Gene expression databases

BgeeiENSBTAG00000007073.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009300.

Structurei

Secondary structure

1423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 31Combined sources6
Helixi36 – 48Combined sources13
Beta strandi49 – 58Combined sources10
Helixi59 – 61Combined sources3
Beta strandi68 – 73Combined sources6
Helixi74 – 76Combined sources3
Helixi77 – 86Combined sources10
Beta strandi91 – 96Combined sources6
Helixi98 – 105Combined sources8
Turni106 – 108Combined sources3
Beta strandi109 – 112Combined sources4
Helixi119 – 121Combined sources3
Helixi126 – 139Combined sources14
Turni141 – 143Combined sources3
Beta strandi144 – 151Combined sources8
Beta strandi157 – 163Combined sources7
Beta strandi173 – 177Combined sources5
Helixi186 – 200Combined sources15
Turni202 – 204Combined sources3
Helixi206 – 214Combined sources9
Beta strandi216 – 221Combined sources6
Helixi225 – 233Combined sources9
Helixi255 – 257Combined sources3
Turni262 – 265Combined sources4
Beta strandi269 – 271Combined sources3
Helixi287 – 298Combined sources12
Turni299 – 302Combined sources4
Beta strandi303 – 310Combined sources8
Beta strandi312 – 319Combined sources8
Beta strandi321 – 325Combined sources5
Helixi330 – 347Combined sources18
Beta strandi354 – 357Combined sources4
Helixi358 – 361Combined sources4
Helixi369 – 376Combined sources8
Beta strandi379 – 385Combined sources7
Beta strandi389 – 392Combined sources4
Helixi398 – 400Combined sources3
Helixi401 – 422Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D4UX-ray1.70A115-423[»]
3DGVX-ray2.50A/B/C23-423[»]
3OSLX-ray6.00A/C23-423[»]
ProteinModelPortaliQ2KIG3.
SMRiQ2KIG3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2KIG3.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 184Substrate bindingBy similarity4
Regioni256 – 257Substrate bindingBy similarity2
Regioni311 – 312Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiQ2KIG3.
KOiK01300.
OMAiLRRNINH.
OrthoDBiEOG091G0HUI.
TreeFamiTF317197.

Family and domain databases

CDDicd06246. M14_CPB2. 1 hit.
Gene3Di3.30.70.340. 1 hit.
InterProiIPR033849. CPB2.
IPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KIG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLYSLGVLV ATVLFCGEHA FAFQRGQVLS ALPRTSRQVQ ILQNVTTTYK
60 70 80 90 100
IVLWQPVAAE YIVKGYEVHF FVNASDVSNV KAHLNASRIP FRVLVENVED
110 120 130 140 150
LIRQQTSNDT ISPRASSSYY EQYHSLNEIY SWIEVMTERY PDMVEKIHIG
160 170 180 190 200
SSYEKYPLYV LKVSKKEQRA KNAMWIDCGI HAREWISPAF CLWFVGSVTY
210 220 230 240 250
YYGKEKMHTN LLKHMDFYIM PVVNVDGYDY TWKKDRMWRK NRSLHEKNAC
260 270 280 290 300
VGTDLNRNFA SKHWCGEGAS SSSCSEIYCG TYPESEPEVK AVADFLRRNI
310 320 330 340 350
KHIKAYISMH SYSQKIVFPY SYSRSRSKDH EELSLVAREA VFAMENIHRN
360 370 380 390 400
IRYTHGSGSE SLYLAPGGSD DWIYDLGIKY SFTFELRDKG KYGFLLPESY
410 420
IRPTCSEALV AVAKIASHVV KNV
Length:423
Mass (Da):48,822
Last modified:March 7, 2006 - v1
Checksum:i361E7FE3F885080E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112649 mRNA. Translation: AAI12650.1.
RefSeqiNP_001039462.1. NM_001045997.2.
UniGeneiBt.93764.

Genome annotation databases

EnsembliENSBTAT00000009300; ENSBTAP00000009300; ENSBTAG00000007073.
GeneIDi508222.
KEGGibta:508222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112649 mRNA. Translation: AAI12650.1.
RefSeqiNP_001039462.1. NM_001045997.2.
UniGeneiBt.93764.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D4UX-ray1.70A115-423[»]
3DGVX-ray2.50A/B/C23-423[»]
3OSLX-ray6.00A/C23-423[»]
ProteinModelPortaliQ2KIG3.
SMRiQ2KIG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009300.

Protein family/group databases

MEROPSiM14.009.

Proteomic databases

PaxDbiQ2KIG3.
PRIDEiQ2KIG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000009300; ENSBTAP00000009300; ENSBTAG00000007073.
GeneIDi508222.
KEGGibta:508222.

Organism-specific databases

CTDi1361.

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiQ2KIG3.
KOiK01300.
OMAiLRRNINH.
OrthoDBiEOG091G0HUI.
TreeFamiTF317197.

Enzyme and pathway databases

BRENDAi3.4.17.20. 908.

Miscellaneous databases

EvolutionaryTraceiQ2KIG3.

Gene expression databases

BgeeiENSBTAG00000007073.

Family and domain databases

CDDicd06246. M14_CPB2. 1 hit.
Gene3Di3.30.70.340. 1 hit.
InterProiIPR033849. CPB2.
IPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCBPB2_BOVIN
AccessioniPrimary (citable) accession number: Q2KIG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 7, 2006
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.