Reviewed, UniProtKB/Swiss-Prot Q2KIG0 (ETFD_BOVIN)

Names and origin

Protein names

Recommended name:
    Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
      Short name=ETF-ubiquinone oxidoreductase
      Short name=ETF-QO
    EC=1.5.5.1
Alternative name(s):
    Electron-transferring-flavoprotein dehydrogenase
      Short name=ETF dehydrogenase

Gene names

Name:

ETFDH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Accepts electrons from ETF and reduces ubiquinone By similarity.
Catalytic activity	Reduced electron-transferring flavoprotein + ubiquinone = electron-transferring flavoprotein + ubiquinol.
Cofactor	Binds 1 4Fe-4S cluster By similarity. FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion inner membrane By similarity.
Sequence similarities	Belongs to the ETF-QO/fixC family. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	4Fe-4S FAD Flavoprotein Iron Iron-sulfur Metal-binding Ubiquinone
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron-transferring-flavoprotein dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion Potential
	Chain	34 – 617	584	Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial		PRO_0000285209
Regions
	Transmembrane	458 – 478	21	Potential
	Domain	577 – 606	30	4Fe-4S ferredoxin-type
	Nucleotide binding	71 – 85	15	FAD Potential
Sites
	Metal binding	561	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	586	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	589	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	592	1	Iron-sulfur (4Fe-4S) Potential
Amino acid modifications
	Modified residue	96	1	N6-acetyllysine By similarity
	Modified residue	153	1	N6-acetyllysine By similarity
	Modified residue	223	1	N6-acetyllysine By similarity
	Modified residue	344	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KIG0-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 3ABDAE292A4C4920	FASTA	617	68,612
	10 20 30 40 50 60 MQVLLARLAC PVYQCFHAIK IKKNYLPLCA TRWSSTSVVP RITTHYTVYP RDQDKRWEGV 70 80 90 100 110 120 NMERFAEEAD VVIVGAGPAG LSAAARLKQL AAQHEKDIRV CLVEKAAQIG AHTLSGACLD 130 140 150 160 170 180 PRALQELFPD WKEKGAPLNT PVTEDRFGIL TEKYRIPVPI LPGLPMNNHG NYIVRLGHLV 190 200 210 220 230 240 SWMGEQAEAL GVEVYPGYAA AEVLFHEDGS VKGIATNDVG IQKDGAPKTT FERGLELHAK 250 260 270 280 290 300 VTIFAEGCHG HLAKQLYRKF DLRANCEPQT YGIGLKELWV IDEKKWKPGR VDHTVGWPLD 310 320 330 340 350 360 RHTYGGSFLY HLNEGEPLVA LGFVVGLDYQ NPYLSPFREF QRWKHHPSIQ PTLEGGKRIA 370 380 390 400 410 420 YGARALNEGG LQCIPKLTFP GGLLIGCSPG FMNVPKIKGT HTAMKSGILA AESIFNQLTN 430 440 450 460 470 480 ENLQSKTIGL DVTEYEDNLK KSWVWKELYA VRNIRPSCHS ILGVYGGMIY TGIFYWIFRG 490 500 510 520 530 540 MEPWTLKHKG SDSDKLKPAK DCTPIEYPKP DGQISFDLLS SVALSGTNHE HDQPAHLTLK 550 560 570 580 590 600 DDSVPVNRNL SIYDGPEQRF CPAGVYEFVP VEQGDGFRLQ INAQNCVHCK TCDIKDPSQN 610 INWVVPEGGG GPAYNGM

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC112652 mRNA. Translation: AAI12653.1.
IPI	IPI00694705.
RefSeq	NP_001070598.1.
UniGene	Bt.42330
3D structure databases
SMR	Q2KIG0. Positions 39-617.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000016679. Bos taurus. [Contig view]
GeneID	768074.
KEGG	bta:768074.
Phylogenomic databases
HOVERGEN	Q2KIG0.
OMA	Q2KIG0. YTPGMEL.
Enzyme and pathway databases
BRENDA	1.5.5.1. 251.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR007859. ETFD_OxRdtase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. [Graphical view]
Pfam	PF05187. ETF_QO. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PROSITE	PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ETFD_BOVIN

Accession

Primary (citable) accession number: Q2KIG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)