Reviewed, UniProtKB/Swiss-Prot Q2KIF8 (SYCM_BOVIN)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase, mitochondrial
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:

CARS2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 555		Cysteinyl-tRNA synthetase, mitochondrial		PRO_0000250740
Regions
	Motif	72 – 82	11	"HIGH" region
	Motif	309 – 313	5	"KMSKS" region
Sites
	Metal binding	70	1	Zinc By similarity
	Metal binding	249	1	Zinc By similarity
	Metal binding	274	1	Zinc By similarity
	Metal binding	278	1	Zinc By similarity
	Binding site	312	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KIF8-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: A55CD4CABBCCF9F7	FASTA	555	61,199
	10 20 30 40 50 60 MLWTRRACGG AWLLRAARGP EPGRSASGAR GQGWLQPTGY DTGVKVYNSL TRRKDPLIVS 70 80 90 100 110 120 RADAASWYSC GPTVYDHAHL GHACSYVRFD IIRRILSRVF GCTVVMVMGI TDVDDKIIRR 130 140 150 160 170 180 ANEMSVSPAS LARLYEEDFK QDMAALKVLP PTAYLRVTEH VPQIVAFIER LIANGHAYCT 190 200 210 220 230 240 AKGNVYFDLQ SRGDRYGKLV GVAPGPVGEP VDSDKRHTSD FALWKAAKPQ EPFWGSPWGD 250 260 270 280 290 300 GRPGWHIECS TIASLVFGSQ LDIHSGGVDL AFPHHENEIA QCEAFHQCPQ WGNYFLHSGH 310 320 330 340 350 360 LHVKGGGEKM SKSLKNYVTI KDFLRSTSPD VFRLFCLRSS YRSAIDYSEG ALLEARSLLR 370 380 390 400 410 420 ATAAFVEDAR AYLRGQLAGG PIQEDVLWER LGRTKAAVQA ALADDFDTVR AVDAVMDLVH 430 440 450 460 470 480 HGNRQLKAAS EEPRGPRSPA VLGAVVASVE HFFETVGVSL AERQCVPGAG SPAALYSLVE 490 500 510 520 530 540 ELVRFRLKVR QFALASGEAP GEARRQRLLE RQPLLEACDA LRRDLAVHGI SIKDRSGSST 550 WELLDPRTED PKPRS

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC112654 mRNA. Translation: AAI12655.1.
IPI	IPI00713506.
RefSeq	NP_001039409.1.
UniGene	Bt.21918
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000011471. Bos taurus. [Contig view]
GeneID	506598.
KEGG	bta:506598.
Phylogenomic databases
HOVERGEN	Q2KIF8.
Enzyme and pathway databases
BRENDA	6.1.1.16. 251.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCM_BOVIN

Accession

Primary (citable) accession number: Q2KIF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)