Q2KIE6 (HMCS2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylglutaryl-CoA synthase, mitochondrial
Short name=HMG-CoA synthase
EC=2.3.3.10

Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase

Gene names

Name:

HMGCS2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.
Catalytic activity	Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the HMG-CoA synthase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Cholesterol metabolism Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: Compara
Molecular_function	hydroxymethylglutaryl-CoA synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Mitochondrion Probable
	Chain	38 – 508	471	Hydroxymethylglutaryl-CoA synthase, mitochondrial		PRO_0000236276
Sites
	Active site	166	1	Potential
Amino acid modifications
	Modified residue	310	1	N6-succinyllysine Ref.2
	Modified residue	433	1	Phosphoserine By similarity
	Modified residue	437	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KIE6 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 12F31AAFBAE986BF	FASTA	508	56,895
	10 20 30 40 50 60 MQRLLTPVRQ VLQVKRVMQE ASLLPARLLP AAHPSFSTVP AVPLAKTDTW PKDVGILAME 70 80 90 100 110 120 VYFPAQYVDQ TELEKFNKVE AGRYTVGLGQ TQMGFCSVQE DVNSLCLTVV QQLMERTQLP 130 140 150 160 170 180 WDSVGRLEVG TETIIDKSKA VKTVLMELFQ DSGNTDIEGI DTTNACYGGT ASLFNAANWM 190 200 210 220 230 240 ESSSWDGRYA LVVCGDIAVY PSGNARPTGG AGAVAMLVGP EAPLVLERGL RGTHMENVYD 250 260 270 280 290 300 FYKPDVTSEY PLVDGKLSIQ CYLRALDKCY AFYRQKIEKQ WKQAGIDRPF TLDDVQYMIF 310 320 330 340 350 360 HTPFCKLVQK SLARLMFNDF LLASGDTQTG IYKGLEAFRG LKLEDTYTNK DVDKAFLKAS 370 380 390 400 410 420 LNMFNKKTKN SLYLSTYNGN MYTSSLYGCL ASLLAHHSAQ DLAGSRIGAF SYGSGLAASF 430 440 450 460 470 480 FSFRVSQDAS PGSPLEKLVS STSDLQKRLA SRKRVSPEEF TEIMNQREQY YHKMNFSPPG 490 500 DKNSLFPGTW YLERVDELYR RKYARRPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.
[2]	"Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase." Du J., Zhou Y., Su X., Yu J.J., Khan S., Jiang H., Kim J., Woo J., Kim J.H., Choi B.H., He B., Chen W., Zhang S., Cerione R.A., Auwerx J., Hao Q., Lin H. Science 334:806-809(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUCCINYLATION AT LYS-310.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC112666 mRNA. Translation: AAI12667.1.
IPI	IPI00742620.
RefSeq	NP_001039348.1. NM_001045883.1.
UniGene	Bt.9689.
3D structure databases
ProteinModelPortal	Q2KIE6.
SMR	Q2KIE6. Positions 53-507.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000005088.
Proteomic databases
PaxDb	Q2KIE6.
PRIDE	Q2KIE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000005088; ENSBTAP00000005088; ENSBTAG00000003898.
GeneID	503684.
KEGG	bta:503684.
Organism-specific databases
CTD	3158.
Phylogenomic databases
eggNOG	COG3425.
GeneTree	ENSGT00390000006096.
HOGENOM	HOG000012351.
HOVERGEN	HBG051912.
InParanoid	Q2KIE6.
KO	K01641.
OMA	LDDLQFM.
OrthoDB	EOG4H72B9.
Enzyme and pathway databases
BioCyc	CATTLE:503684-MONOMER.
UniPathway	UPA00058; UER00102.
Family and domain databases
Gene3D	3.40.47.10. 1 hit.
InterPro	IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITE	PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20866256.

Entry information

Entry name

HMCS2_BOVIN

Accession

Primary (citable) accession number: Q2KIE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 7, 2006
Last modified:	May 29, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program