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Q2KIE6

- HMCS2_BOVIN

UniProt

Q2KIE6 - HMCS2_BOVIN

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Protein

Hydroxymethylglutaryl-CoA synthase, mitochondrial

Gene
HMGCS2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801Substrate By similarity
Active sitei132 – 1321Proton donor/acceptor By similarity
Active sitei166 – 1661Acyl-thioester intermediate By similarity
Binding sitei204 – 2041Substrate By similarity
Binding sitei258 – 2581Substrate By similarity
Active sitei301 – 3011Proton donor/acceptor By similarity
Binding sitei380 – 3801Substrate By similarity

GO - Molecular functioni

  1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. cholesterol biosynthetic process Source: UniProtKB-KW
  2. isoprenoid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

ReactomeiREACT_207237. PPARA activates gene expression.
REACT_212702. Synthesis of Ketone Bodies.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase, mitochondrial (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:HMGCS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 3

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737Mitochondrion InferredAdd
BLAST
Chaini38 – 508471Hydroxymethylglutaryl-CoA synthase, mitochondrialPRO_0000236276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521N6-succinyllysine By similarity
Modified residuei243 – 2431N6-acetyllysine By similarity
Modified residuei256 – 2561N6-acetyllysine; alternate By similarity
Modified residuei256 – 2561N6-succinyllysine; alternate By similarity
Modified residuei306 – 3061N6-acetyllysine By similarity
Modified residuei310 – 3101N6-acetyllysine; alternate By similarity
Modified residuei310 – 3101N6-succinyllysine; alternate1 Publication
Modified residuei333 – 3331N6-succinyllysine By similarity
Modified residuei342 – 3421N6-acetyllysine; alternate By similarity
Modified residuei342 – 3421N6-succinyllysine; alternate By similarity
Modified residuei350 – 3501N6-acetyllysine; alternate By similarity
Modified residuei350 – 3501N6-succinyllysine; alternate By similarity
Modified residuei354 – 3541N6-acetyllysine; alternate By similarity
Modified residuei354 – 3541N6-succinyllysine; alternate By similarity
Modified residuei358 – 3581N6-acetyllysine; alternate By similarity
Modified residuei358 – 3581N6-succinyllysine; alternate By similarity
Modified residuei437 – 4371N6-acetyllysine By similarity
Modified residuei447 – 4471N6-acetyllysine; alternate By similarity
Modified residuei447 – 4471N6-succinyllysine; alternate By similarity
Modified residuei473 – 4731N6-acetyllysine; alternate By similarity
Modified residuei473 – 4731N6-succinyllysine; alternate By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ2KIE6.
PRIDEiQ2KIE6.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005088.

Structurei

3D structure databases

ProteinModelPortaliQ2KIE6.
SMRiQ2KIE6. Positions 53-507.

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3425.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
HOVERGENiHBG051912.
InParanoidiQ2KIE6.
KOiK01641.
OMAiMLVGPEA.
OrthoDBiEOG741Z1W.
TreeFamiTF105361.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KIE6-1 [UniParc]FASTAAdd to Basket

« Hide

MQRLLTPVRQ VLQVKRVMQE ASLLPARLLP AAHPSFSTVP AVPLAKTDTW    50
PKDVGILAME VYFPAQYVDQ TELEKFNKVE AGRYTVGLGQ TQMGFCSVQE 100
DVNSLCLTVV QQLMERTQLP WDSVGRLEVG TETIIDKSKA VKTVLMELFQ 150
DSGNTDIEGI DTTNACYGGT ASLFNAANWM ESSSWDGRYA LVVCGDIAVY 200
PSGNARPTGG AGAVAMLVGP EAPLVLERGL RGTHMENVYD FYKPDVTSEY 250
PLVDGKLSIQ CYLRALDKCY AFYRQKIEKQ WKQAGIDRPF TLDDVQYMIF 300
HTPFCKLVQK SLARLMFNDF LLASGDTQTG IYKGLEAFRG LKLEDTYTNK 350
DVDKAFLKAS LNMFNKKTKN SLYLSTYNGN MYTSSLYGCL ASLLAHHSAQ 400
DLAGSRIGAF SYGSGLAASF FSFRVSQDAS PGSPLEKLVS STSDLQKRLA 450
SRKRVSPEEF TEIMNQREQY YHKMNFSPPG DKNSLFPGTW YLERVDELYR 500
RKYARRPV 508
Length:508
Mass (Da):56,895
Last modified:March 7, 2006 - v1
Checksum:i12F31AAFBAE986BF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC112666 mRNA. Translation: AAI12667.1.
RefSeqiNP_001039348.1. NM_001045883.1.
UniGeneiBt.9689.

Genome annotation databases

EnsembliENSBTAT00000005088; ENSBTAP00000005088; ENSBTAG00000003898.
GeneIDi503684.
KEGGibta:503684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC112666 mRNA. Translation: AAI12667.1 .
RefSeqi NP_001039348.1. NM_001045883.1.
UniGenei Bt.9689.

3D structure databases

ProteinModelPortali Q2KIE6.
SMRi Q2KIE6. Positions 53-507.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000005088.

Proteomic databases

PaxDbi Q2KIE6.
PRIDEi Q2KIE6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000005088 ; ENSBTAP00000005088 ; ENSBTAG00000003898 .
GeneIDi 503684.
KEGGi bta:503684.

Organism-specific databases

CTDi 3158.

Phylogenomic databases

eggNOGi COG3425.
GeneTreei ENSGT00390000006096.
HOGENOMi HOG000012351.
HOVERGENi HBG051912.
InParanoidi Q2KIE6.
KOi K01641.
OMAi MLVGPEA.
OrthoDBi EOG741Z1W.
TreeFami TF105361.

Enzyme and pathway databases

UniPathwayi UPA00058 ; UER00102 .
Reactomei REACT_207237. PPARA activates gene expression.
REACT_212702. Synthesis of Ketone Bodies.

Miscellaneous databases

NextBioi 20866256.

Family and domain databases

Gene3Di 3.40.47.10. 1 hit.
InterProi IPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 3 hits.
TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Testis.
  2. "Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase."
    Du J., Zhou Y., Su X., Yu J.J., Khan S., Jiang H., Kim J., Woo J., Kim J.H., Choi B.H., He B., Chen W., Zhang S., Cerione R.A., Auwerx J., Hao Q., Lin H.
    Science 334:806-809(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION AT LYS-310.

Entry informationi

Entry nameiHMCS2_BOVIN
AccessioniPrimary (citable) accession number: Q2KIE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 7, 2006
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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