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Q2KIE6

- HMCS2_BOVIN

UniProt

Q2KIE6 - HMCS2_BOVIN

Protein

Hydroxymethylglutaryl-CoA synthase, mitochondrial

Gene

HMGCS2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

    Catalytic activityi

    Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801SubstrateBy similarity
    Active sitei132 – 1321Proton donor/acceptorPROSITE-ProRule annotation
    Active sitei166 – 1661Acyl-thioester intermediatePROSITE-ProRule annotation
    Binding sitei204 – 2041SubstrateBy similarity
    Binding sitei258 – 2581SubstrateBy similarity
    Active sitei301 – 3011Proton donor/acceptorPROSITE-ProRule annotation
    Binding sitei380 – 3801SubstrateBy similarity

    GO - Molecular functioni

    1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cholesterol biosynthetic process Source: UniProtKB-KW
    2. isoprenoid biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Enzyme and pathway databases

    ReactomeiREACT_207237. PPARA activates gene expression.
    REACT_212702. Synthesis of Ketone Bodies.
    UniPathwayiUPA00058; UER00102.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylglutaryl-CoA synthase, mitochondrial (EC:2.3.3.10)
    Short name:
    HMG-CoA synthase
    Alternative name(s):
    3-hydroxy-3-methylglutaryl coenzyme A synthase
    Gene namesi
    Name:HMGCS2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 3

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: Ensembl

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3737MitochondrionCuratedAdd
    BLAST
    Chaini38 – 508471Hydroxymethylglutaryl-CoA synthase, mitochondrialPRO_0000236276Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei52 – 521N6-succinyllysineBy similarity
    Modified residuei243 – 2431N6-acetyllysineBy similarity
    Modified residuei256 – 2561N6-acetyllysine; alternateBy similarity
    Modified residuei256 – 2561N6-succinyllysine; alternateBy similarity
    Modified residuei306 – 3061N6-acetyllysineBy similarity
    Modified residuei310 – 3101N6-acetyllysine; alternateBy similarity
    Modified residuei310 – 3101N6-succinyllysine; alternate1 Publication
    Modified residuei333 – 3331N6-succinyllysineBy similarity
    Modified residuei342 – 3421N6-acetyllysine; alternateBy similarity
    Modified residuei342 – 3421N6-succinyllysine; alternateBy similarity
    Modified residuei350 – 3501N6-acetyllysine; alternateBy similarity
    Modified residuei350 – 3501N6-succinyllysine; alternateBy similarity
    Modified residuei354 – 3541N6-acetyllysine; alternateBy similarity
    Modified residuei354 – 3541N6-succinyllysine; alternateBy similarity
    Modified residuei358 – 3581N6-acetyllysine; alternateBy similarity
    Modified residuei358 – 3581N6-succinyllysine; alternateBy similarity
    Modified residuei437 – 4371N6-acetyllysineBy similarity
    Modified residuei447 – 4471N6-acetyllysine; alternateBy similarity
    Modified residuei447 – 4471N6-succinyllysine; alternateBy similarity
    Modified residuei473 – 4731N6-acetyllysine; alternateBy similarity
    Modified residuei473 – 4731N6-succinyllysine; alternateBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ2KIE6.
    PRIDEiQ2KIE6.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000005088.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2KIE6.
    SMRiQ2KIE6. Positions 53-507.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HMG-CoA synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3425.
    GeneTreeiENSGT00390000006096.
    HOGENOMiHOG000012351.
    HOVERGENiHBG051912.
    InParanoidiQ2KIE6.
    KOiK01641.
    OMAiMLVGPEA.
    OrthoDBiEOG741Z1W.
    TreeFamiTF105361.

    Family and domain databases

    Gene3Di3.40.47.10. 1 hit.
    InterProiIPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 3 hits.
    TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2KIE6-1 [UniParc]FASTAAdd to Basket

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    MQRLLTPVRQ VLQVKRVMQE ASLLPARLLP AAHPSFSTVP AVPLAKTDTW    50
    PKDVGILAME VYFPAQYVDQ TELEKFNKVE AGRYTVGLGQ TQMGFCSVQE 100
    DVNSLCLTVV QQLMERTQLP WDSVGRLEVG TETIIDKSKA VKTVLMELFQ 150
    DSGNTDIEGI DTTNACYGGT ASLFNAANWM ESSSWDGRYA LVVCGDIAVY 200
    PSGNARPTGG AGAVAMLVGP EAPLVLERGL RGTHMENVYD FYKPDVTSEY 250
    PLVDGKLSIQ CYLRALDKCY AFYRQKIEKQ WKQAGIDRPF TLDDVQYMIF 300
    HTPFCKLVQK SLARLMFNDF LLASGDTQTG IYKGLEAFRG LKLEDTYTNK 350
    DVDKAFLKAS LNMFNKKTKN SLYLSTYNGN MYTSSLYGCL ASLLAHHSAQ 400
    DLAGSRIGAF SYGSGLAASF FSFRVSQDAS PGSPLEKLVS STSDLQKRLA 450
    SRKRVSPEEF TEIMNQREQY YHKMNFSPPG DKNSLFPGTW YLERVDELYR 500
    RKYARRPV 508
    Length:508
    Mass (Da):56,895
    Last modified:March 7, 2006 - v1
    Checksum:i12F31AAFBAE986BF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC112666 mRNA. Translation: AAI12667.1.
    RefSeqiNP_001039348.1. NM_001045883.1.
    UniGeneiBt.9689.

    Genome annotation databases

    EnsembliENSBTAT00000005088; ENSBTAP00000005088; ENSBTAG00000003898.
    GeneIDi503684.
    KEGGibta:503684.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC112666 mRNA. Translation: AAI12667.1 .
    RefSeqi NP_001039348.1. NM_001045883.1.
    UniGenei Bt.9689.

    3D structure databases

    ProteinModelPortali Q2KIE6.
    SMRi Q2KIE6. Positions 53-507.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000005088.

    Proteomic databases

    PaxDbi Q2KIE6.
    PRIDEi Q2KIE6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000005088 ; ENSBTAP00000005088 ; ENSBTAG00000003898 .
    GeneIDi 503684.
    KEGGi bta:503684.

    Organism-specific databases

    CTDi 3158.

    Phylogenomic databases

    eggNOGi COG3425.
    GeneTreei ENSGT00390000006096.
    HOGENOMi HOG000012351.
    HOVERGENi HBG051912.
    InParanoidi Q2KIE6.
    KOi K01641.
    OMAi MLVGPEA.
    OrthoDBi EOG741Z1W.
    TreeFami TF105361.

    Enzyme and pathway databases

    UniPathwayi UPA00058 ; UER00102 .
    Reactomei REACT_207237. PPARA activates gene expression.
    REACT_212702. Synthesis of Ketone Bodies.

    Miscellaneous databases

    NextBioi 20866256.

    Family and domain databases

    Gene3Di 3.40.47.10. 1 hit.
    InterProi IPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 3 hits.
    TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Testis.
    2. "Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase."
      Du J., Zhou Y., Su X., Yu J.J., Khan S., Jiang H., Kim J., Woo J., Kim J.H., Choi B.H., He B., Chen W., Zhang S., Cerione R.A., Auwerx J., Hao Q., Lin H.
      Science 334:806-809(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION AT LYS-310.

    Entry informationi

    Entry nameiHMCS2_BOVIN
    AccessioniPrimary (citable) accession number: Q2KIE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3