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Q2KIE6

- HMCS2_BOVIN

UniProt

Q2KIE6 - HMCS2_BOVIN

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Protein

Hydroxymethylglutaryl-CoA synthase, mitochondrial

Gene

HMGCS2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801SubstrateBy similarity
Active sitei132 – 1321Proton donor/acceptorPROSITE-ProRule annotation
Active sitei166 – 1661Acyl-thioester intermediatePROSITE-ProRule annotation
Binding sitei204 – 2041SubstrateBy similarity
Binding sitei258 – 2581SubstrateBy similarity
Active sitei301 – 3011Proton donor/acceptorPROSITE-ProRule annotation
Binding sitei380 – 3801SubstrateBy similarity

GO - Molecular functioni

  1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. cholesterol biosynthetic process Source: UniProtKB-KW
  2. isoprenoid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

ReactomeiREACT_207237. PPARA activates gene expression.
REACT_212702. Synthesis of Ketone Bodies.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase, mitochondrial (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:HMGCS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 3

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737MitochondrionCuratedAdd
BLAST
Chaini38 – 508471Hydroxymethylglutaryl-CoA synthase, mitochondrialPRO_0000236276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521N6-succinyllysineBy similarity
Modified residuei243 – 2431N6-acetyllysineBy similarity
Modified residuei256 – 2561N6-acetyllysine; alternateBy similarity
Modified residuei256 – 2561N6-succinyllysine; alternateBy similarity
Modified residuei306 – 3061N6-acetyllysineBy similarity
Modified residuei310 – 3101N6-acetyllysine; alternateBy similarity
Modified residuei310 – 3101N6-succinyllysine; alternate1 Publication
Modified residuei333 – 3331N6-succinyllysineBy similarity
Modified residuei342 – 3421N6-acetyllysine; alternateBy similarity
Modified residuei342 – 3421N6-succinyllysine; alternateBy similarity
Modified residuei350 – 3501N6-acetyllysine; alternateBy similarity
Modified residuei350 – 3501N6-succinyllysine; alternateBy similarity
Modified residuei354 – 3541N6-acetyllysine; alternateBy similarity
Modified residuei354 – 3541N6-succinyllysine; alternateBy similarity
Modified residuei358 – 3581N6-acetyllysine; alternateBy similarity
Modified residuei358 – 3581N6-succinyllysine; alternateBy similarity
Modified residuei437 – 4371N6-acetyllysineBy similarity
Modified residuei447 – 4471N6-acetyllysine; alternateBy similarity
Modified residuei447 – 4471N6-succinyllysine; alternateBy similarity
Modified residuei473 – 4731N6-acetyllysine; alternateBy similarity
Modified residuei473 – 4731N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ2KIE6.
PRIDEiQ2KIE6.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005088.

Structurei

3D structure databases

ProteinModelPortaliQ2KIE6.
SMRiQ2KIE6. Positions 53-507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3425.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
HOVERGENiHBG051912.
InParanoidiQ2KIE6.
KOiK01641.
OMAiMLVGPEA.
OrthoDBiEOG741Z1W.
TreeFamiTF105361.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KIE6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQRLLTPVRQ VLQVKRVMQE ASLLPARLLP AAHPSFSTVP AVPLAKTDTW
60 70 80 90 100
PKDVGILAME VYFPAQYVDQ TELEKFNKVE AGRYTVGLGQ TQMGFCSVQE
110 120 130 140 150
DVNSLCLTVV QQLMERTQLP WDSVGRLEVG TETIIDKSKA VKTVLMELFQ
160 170 180 190 200
DSGNTDIEGI DTTNACYGGT ASLFNAANWM ESSSWDGRYA LVVCGDIAVY
210 220 230 240 250
PSGNARPTGG AGAVAMLVGP EAPLVLERGL RGTHMENVYD FYKPDVTSEY
260 270 280 290 300
PLVDGKLSIQ CYLRALDKCY AFYRQKIEKQ WKQAGIDRPF TLDDVQYMIF
310 320 330 340 350
HTPFCKLVQK SLARLMFNDF LLASGDTQTG IYKGLEAFRG LKLEDTYTNK
360 370 380 390 400
DVDKAFLKAS LNMFNKKTKN SLYLSTYNGN MYTSSLYGCL ASLLAHHSAQ
410 420 430 440 450
DLAGSRIGAF SYGSGLAASF FSFRVSQDAS PGSPLEKLVS STSDLQKRLA
460 470 480 490 500
SRKRVSPEEF TEIMNQREQY YHKMNFSPPG DKNSLFPGTW YLERVDELYR

RKYARRPV
Length:508
Mass (Da):56,895
Last modified:March 7, 2006 - v1
Checksum:i12F31AAFBAE986BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112666 mRNA. Translation: AAI12667.1.
RefSeqiNP_001039348.1. NM_001045883.1.
UniGeneiBt.9689.

Genome annotation databases

EnsembliENSBTAT00000005088; ENSBTAP00000005088; ENSBTAG00000003898.
GeneIDi503684.
KEGGibta:503684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112666 mRNA. Translation: AAI12667.1 .
RefSeqi NP_001039348.1. NM_001045883.1.
UniGenei Bt.9689.

3D structure databases

ProteinModelPortali Q2KIE6.
SMRi Q2KIE6. Positions 53-507.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000005088.

Proteomic databases

PaxDbi Q2KIE6.
PRIDEi Q2KIE6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000005088 ; ENSBTAP00000005088 ; ENSBTAG00000003898 .
GeneIDi 503684.
KEGGi bta:503684.

Organism-specific databases

CTDi 3158.

Phylogenomic databases

eggNOGi COG3425.
GeneTreei ENSGT00390000006096.
HOGENOMi HOG000012351.
HOVERGENi HBG051912.
InParanoidi Q2KIE6.
KOi K01641.
OMAi MLVGPEA.
OrthoDBi EOG741Z1W.
TreeFami TF105361.

Enzyme and pathway databases

UniPathwayi UPA00058 ; UER00102 .
Reactomei REACT_207237. PPARA activates gene expression.
REACT_212702. Synthesis of Ketone Bodies.

Miscellaneous databases

NextBioi 20866256.

Family and domain databases

Gene3Di 3.40.47.10. 1 hit.
InterProi IPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 3 hits.
TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Testis.
  2. "Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase."
    Du J., Zhou Y., Su X., Yu J.J., Khan S., Jiang H., Kim J., Woo J., Kim J.H., Choi B.H., He B., Chen W., Zhang S., Cerione R.A., Auwerx J., Hao Q., Lin H.
    Science 334:806-809(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION AT LYS-310.

Entry informationi

Entry nameiHMCS2_BOVIN
AccessioniPrimary (citable) accession number: Q2KIE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 7, 2006
Last modified: October 1, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3