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Q2KID2

- NTH_BOVIN

UniProt

Q2KID2 - NTH_BOVIN

Protein

Endonuclease III-like protein 1

Gene

NTHL1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei213 – 2131Nucleophile; for N-glycosylase activityUniRule annotation
    Sitei232 – 2321Important for catalytic activityUniRule annotation
    Metal bindingi283 – 2831Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi290 – 2901Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi293 – 2931Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi299 – 2991Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
    3. DNA N-glycosylase activity Source: Ensembl
    4. double-stranded DNA binding Source: Ensembl
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. base-excision repair, AP site formation Source: Ensembl
    2. nucleotide-excision repair, DNA incision, 5'-to lesion Source: Ensembl

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_203635. Displacement of DNA glycosylase by APE1.
    REACT_227493. Recognition and association of DNA glycosylase with site containing an affected pyrimidine.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease III-like protein 1UniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
    Alternative name(s):
    Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
    Short name:
    DNA glycoslyase/AP lyaseUniRule annotation
    Gene namesi
    Name:NTHL1UniRule annotation
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 25

    Subcellular locationi

    Nucleus UniRule annotation. Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2828MitochondrionUniRule annotationAdd
    BLAST
    Chaini29 – 305277Endonuclease III-like protein 1PRO_0000244406Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei64 – 641PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ2KID2.

    Interactioni

    Subunit structurei

    Interacts with YBX1.UniRule annotation

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000046623.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2KID2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini192 – 21625HhHUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nth/MutY family.UniRule annotation
    Contains 1 HhH domain.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0177.
    GeneTreeiENSGT00510000047513.
    HOGENOMiHOG000252209.
    HOVERGENiHBG052675.
    InParanoidiQ2KID2.
    KOiK10773.
    OMAiWRNKVKY.
    OrthoDBiEOG76MK8Z.
    TreeFamiTF314967.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_03183. Endonuclease_III_Nth.
    InterProiIPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR023170. HTH_base_excis_C.
    [Graphical view]
    PfamiPF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view]
    SMARTiSM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
    PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2KID2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNAAGVRMVV TRARSRGTGA SLRRRGEKAA PLRSGEAAAE ERKSYSPVKR    50
    RRKAQRLSVA YEASEGEGGE GAEHLQAPSW QPQDWRQQLD NIRTMRSGKD 100
    APVDQLGAEH CFDPSASPKV RRYQVLLSLM LSSQTKDQVT AGAMQRLRAR 150
    GLTVDSILQT DDSTLGALIY PVGFWRSKVK YIKQTSAILQ QRYDGDIPAS 200
    VAELVALPGV GPKMAHLAMA VAWGTVSGIA VDTHVHRIAN RLRWTKKATK 250
    SPEETRRALE EWLPRELWSE INGLLVGFGQ QTCLPIRPRC QACLNRALCP 300
    AARGL 305
    Length:305
    Mass (Da):33,645
    Last modified:March 7, 2006 - v1
    Checksum:i9318553C857F500C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GJ062847 Genomic DNA. Translation: DAA15605.1.
    BC112681 mRNA. Translation: AAI12682.1.
    RefSeqiNP_001039862.1. NM_001046397.2.
    UniGeneiBt.6550.

    Genome annotation databases

    EnsembliENSBTAT00000049780; ENSBTAP00000046623; ENSBTAG00000006272.
    GeneIDi535203.
    KEGGibta:535203.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GJ062847 Genomic DNA. Translation: DAA15605.1 .
    BC112681 mRNA. Translation: AAI12682.1 .
    RefSeqi NP_001039862.1. NM_001046397.2.
    UniGenei Bt.6550.

    3D structure databases

    ProteinModelPortali Q2KID2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000046623.

    Proteomic databases

    PRIDEi Q2KID2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000049780 ; ENSBTAP00000046623 ; ENSBTAG00000006272 .
    GeneIDi 535203.
    KEGGi bta:535203.

    Organism-specific databases

    CTDi 4913.

    Phylogenomic databases

    eggNOGi COG0177.
    GeneTreei ENSGT00510000047513.
    HOGENOMi HOG000252209.
    HOVERGENi HBG052675.
    InParanoidi Q2KID2.
    KOi K10773.
    OMAi WRNKVKY.
    OrthoDBi EOG76MK8Z.
    TreeFami TF314967.

    Enzyme and pathway databases

    Reactomei REACT_203635. Displacement of DNA glycosylase by APE1.
    REACT_227493. Recognition and association of DNA glycosylase with site containing an affected pyrimidine.

    Miscellaneous databases

    NextBioi 20876660.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_03183. Endonuclease_III_Nth.
    InterProi IPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR023170. HTH_base_excis_C.
    [Graphical view ]
    Pfami PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    SMARTi SM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    PROSITEi PS00764. ENDONUCLEASE_III_1. 1 hit.
    PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hereford.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Hypothalamus.

    Entry informationi

    Entry nameiNTH_BOVIN
    AccessioniPrimary (citable) accession number: Q2KID2
    Secondary accession number(s): M5FK15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3