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Protein

Endonuclease III-like protein 1

Gene

NTHL1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei213Nucleophile; for N-glycosylase activityUniRule annotation1
Sitei232Important for catalytic activityUniRule annotation1
Metal bindingi283Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi290Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi293Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi299Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-110329 Cleavage of the damaged pyrimidine
R-BTA-110357 Displacement of DNA glycosylase by APEX1

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease III-like protein 1UniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
Alternative name(s):
Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Short name:
DNA glycosylase/AP lyaseUniRule annotation
Gene namesi
Name:NTHL1UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Organism-specific databases

VGNCiVGNC:32301 NTHL1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionUniRule annotationAdd BLAST28
ChainiPRO_000024440629 – 305Endonuclease III-like protein 1Add BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2KID2
PRIDEiQ2KID2

Expressioni

Gene expression databases

BgeeiENSBTAG00000006272

Interactioni

Subunit structurei

Interacts with YBX1.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000046623

Structurei

3D structure databases

ProteinModelPortaliQ2KID2
SMRiQ2KID2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 216HhHUniRule annotationAdd BLAST25

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1921 Eukaryota
COG0177 LUCA
GeneTreeiENSGT00510000047513
HOGENOMiHOG000252209
HOVERGENiHBG052675
InParanoidiQ2KID2
KOiK10773
OMAiWQQFTHL
OrthoDBiEOG091G0IVB
TreeFamiTF314967

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_03183 Endonuclease_III_Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR030841 NTH1
PfamiView protein in Pfam
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KID2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAAGVRMVV TRARSRGTGA SLRRRGEKAA PLRSGEAAAE ERKSYSPVKR
60 70 80 90 100
RRKAQRLSVA YEASEGEGGE GAEHLQAPSW QPQDWRQQLD NIRTMRSGKD
110 120 130 140 150
APVDQLGAEH CFDPSASPKV RRYQVLLSLM LSSQTKDQVT AGAMQRLRAR
160 170 180 190 200
GLTVDSILQT DDSTLGALIY PVGFWRSKVK YIKQTSAILQ QRYDGDIPAS
210 220 230 240 250
VAELVALPGV GPKMAHLAMA VAWGTVSGIA VDTHVHRIAN RLRWTKKATK
260 270 280 290 300
SPEETRRALE EWLPRELWSE INGLLVGFGQ QTCLPIRPRC QACLNRALCP

AARGL
Length:305
Mass (Da):33,645
Last modified:March 7, 2006 - v1
Checksum:i9318553C857F500C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GJ062847 Genomic DNA Translation: DAA15605.1
BC112681 mRNA Translation: AAI12682.1
RefSeqiNP_001039862.1, NM_001046397.2
UniGeneiBt.6550

Genome annotation databases

EnsembliENSBTAT00000049780; ENSBTAP00000046623; ENSBTAG00000006272
GeneIDi535203
KEGGibta:535203

Similar proteinsi

Entry informationi

Entry nameiNTH_BOVIN
AccessioniPrimary (citable) accession number: Q2KID2
Secondary accession number(s): M5FK15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 7, 2006
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health