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Q2KID2 (NTHL1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease III-like protein 1
EC=4.2.99.18
Gene names
Name:NTHL1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions By similarity.

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is not important for the catalytic activity, but is probably involved in the proper positioning of the enzyme along the DNA strand By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the Nth/MutY family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305Endonuclease III-like protein 1
PRO_0000244406

Sites

Active site2131Nucleophile; for N-glycosylase activity By similarity
Metal binding2831Iron-sulfur (4Fe-4S) By similarity
Metal binding2901Iron-sulfur (4Fe-4S) By similarity
Metal binding2931Iron-sulfur (4Fe-4S) By similarity
Metal binding2991Iron-sulfur (4Fe-4S) By similarity

Amino acid modifications

Modified residue641Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2KID2 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 9318553C857F500C

FASTA30533,645
        10         20         30         40         50         60 
MNAAGVRMVV TRARSRGTGA SLRRRGEKAA PLRSGEAAAE ERKSYSPVKR RRKAQRLSVA 

        70         80         90        100        110        120 
YEASEGEGGE GAEHLQAPSW QPQDWRQQLD NIRTMRSGKD APVDQLGAEH CFDPSASPKV 

       130        140        150        160        170        180 
RRYQVLLSLM LSSQTKDQVT AGAMQRLRAR GLTVDSILQT DDSTLGALIY PVGFWRSKVK 

       190        200        210        220        230        240 
YIKQTSAILQ QRYDGDIPAS VAELVALPGV GPKMAHLAMA VAWGTVSGIA VDTHVHRIAN 

       250        260        270        280        290        300 
RLRWTKKATK SPEETRRALE EWLPRELWSE INGLLVGFGQ QTCLPIRPRC QACLNRALCP 


AARGL

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Hypothalamus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC112681 mRNA. Translation: AAI12682.1.
IPIIPI00689580.
RefSeqNP_001039862.1. NM_001046397.2.
UniGeneBt.6550.

3D structure databases

ProteinModelPortalQ2KID2.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000046623.

Proteomic databases

PRIDEQ2KID2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000049780; ENSBTAP00000046623; ENSBTAG00000006272.
GeneID535203.
KEGGbta:535203.

Organism-specific databases

CTD4913.

Phylogenomic databases

eggNOGCOG0177.
GeneTreeENSGT00510000047513.
HOGENOMHOG000252209.
HOVERGENHBG052675.
InParanoidQ2KID2.
KOK10773.
OMACLNQALC.
OrthoDBEOG4933JH.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
[Graphical view]
SUPFAMSSF48150. DNA_glycsylse. 1 hit.
PROSITEPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20876660.

Entry information

Entry nameNTHL1_BOVIN
AccessionPrimary (citable) accession number: Q2KID2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 7, 2006
Last modified: April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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