Q2KIC5 (ITPA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inosine triphosphate pyrophosphatase
Short name=ITPase
Short name=Inosine triphosphatase
EC=3.6.1.19

Alternative name(s):
Non-canonical purine NTP pyrophosphatase
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Name:

ITPA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions By similarity.
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate.
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 208	207	Inosine triphosphate pyrophosphatase		PRO_0000306869
Regions
	Region	14 – 19	6	Substrate binding By similarity
	Region	72 – 73	2	Substrate binding By similarity
	Region	149 – 152	4	Substrate binding By similarity
	Region	177 – 178	2	Substrate binding By similarity
Sites
	Metal binding	44	1	Magnesium or manganese By similarity
	Metal binding	72	1	Magnesium or manganese By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KIC5 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: F49F922F60A8822B	FASTA	208	23,061
	10 20 30 40 50 60 MAASLAGKKI VFVTGNAKKL EEVIQILGDK FPCTLVAQKI DLPEYQGEPD EISIRKCQEA 70 80 90 100 110 120 ARQVQGPVLV EDTCLCFNAL GGLPGPYIKW FLEKLKPEGL HQLLEGFQDK SAYALCTFAF 130 140 150 160 170 180 STGDPNEPVR LFRGRTMGRI VVPRGCRDFG WDPCFQPDGY EQTYAEMPKA EKNTISHRFR 190 200 ALLALQEYFS SLTPGVGDDH PSWGSGEG

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC112688 mRNA. Translation: AAI12689.1.
IPI	IPI00702859.
RefSeq	NP_001069750.1. NM_001076282.1.
UniGene	Bt.53005.
3D structure databases
ProteinModelPortal	Q2KIC5.
SMR	Q2KIC5. Positions 1-192.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KIC5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000018301; ENSBTAP00000018301; ENSBTAG00000013776.
GeneID	613653.
KEGG	bta:613653.
Organism-specific databases
CTD	3704.
Phylogenomic databases
eggNOG	maNOG04379.
GeneTree	ENSGT00390000015399.
HOVERGEN	HBG039521.
InParanoid	Q2KIC5.
OrthoDB	EOG4THVV4.
PhylomeDB	Q2KIC5.
Family and domain databases
InterPro	IPR002637. Ham1p-like. [Graphical view]
KO	K01519.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ITPA_BOVIN

Accession

Primary (citable) accession number: Q2KIC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	March 7, 2006
Last modified:	November 16, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program