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Protein

Mitochondrial enolase superfamily member 1

Gene

ENOSF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. May down-regulate thymidylate synthase activity, possibly already at the RNA level, by promoting the degradation of TYMS mRNA via an antisense RNA-based mechanism.By similarity

Catalytic activityi

L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341SubstrateBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Active sitei222 – 2221Proton donor/acceptorBy similarity
Metal bindingi250 – 2501MagnesiumBy similarity
Binding sitei252 – 2521SubstrateBy similarity
Metal bindingi276 – 2761MagnesiumBy similarity
Binding sitei276 – 2761SubstrateBy similarity
Metal bindingi305 – 3051MagnesiumBy similarity
Binding sitei305 – 3051SubstrateBy similarity
Active sitei355 – 3551Sequence analysis
Binding sitei386 – 3861SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial enolase superfamily member 1 (EC:4.2.1.68By similarity)
Alternative name(s):
L-fuconate dehydratase
Gene namesi
Name:ENOSF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Mitochondrial enolase superfamily member 1PRO_0000331651Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei148 – 1481PhosphoserineBy similarity

Post-translational modificationi

Could be sumoylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ2KIA9.
PRIDEiQ2KIA9.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000031013.

Structurei

3D structure databases

ProteinModelPortaliQ2KIA9.
SMRiQ2KIA9. Positions 5-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 263Substrate bindingBy similarity
Regioni355 – 3573Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IET8. Eukaryota.
COG4948. LUCA.
GeneTreeiENSGT00390000014290.
HOGENOMiHOG000113757.
HOVERGENiHBG053904.
InParanoidiQ2KIA9.
KOiK18334.
OMAiSAAYCIL.
OrthoDBiEOG77DJ5S.
TreeFamiTF300529.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 2 hits.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiPS00909. MR_MLE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KIA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHGRVSRLS VHDVRFPTSL GGHGSDAMHT DPDYSAAYVV LETDAEDGLK
60 70 80 90 100
GYGITFTLGR GTEVVVCAVN ALAPHVLNKD LGEIVGDFRG FYRQLTSDGQ
110 120 130 140 150
LRWIGPEKGV VHLATAAVLN AVWDLWAKQE GKPLWKLLVD MDPRTLVSCI
160 170 180 190 200
DFRYITDVLT EEEACEILRQ SQVGKKEREE QMLAHGYPAY TTSCAWLGYP
210 220 230 240 250
DATLKQLCSE ALKDGWTRFK VKVGADLQDD IRRCRLVRNM IGPEKTLMMD
260 270 280 290 300
ANQRWDVPEA VEWMTKLAEF KPLWIEEPTS PDDILGHAAI SKALAPLGIG
310 320 330 340 350
VATGEQCHNR VIFKQLLQAK ALKFLQIDSC RLGSVNENLS VLLMAKKFEI
360 370 380 390 400
PVCPHAGGVG LCELVQHLII FDFISVSASL QDRMCEYVDH LHEHFKYPVL
410 420 430 440
IREAAYMPPK DAGYSTEMKE DSVKRHRYPD GEVWKKLLSA QGN
Length:443
Mass (Da):49,621
Last modified:March 7, 2006 - v1
Checksum:iC3D921D57C410AE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112706 mRNA. Translation: AAI12707.1.
RefSeqiNP_001040015.1. NM_001046550.2.
UniGeneiBt.59052.

Genome annotation databases

EnsembliENSBTAT00000031050; ENSBTAP00000031013; ENSBTAG00000007000.
GeneIDi615106.
KEGGibta:615106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112706 mRNA. Translation: AAI12707.1.
RefSeqiNP_001040015.1. NM_001046550.2.
UniGeneiBt.59052.

3D structure databases

ProteinModelPortaliQ2KIA9.
SMRiQ2KIA9. Positions 5-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000031013.

Proteomic databases

PaxDbiQ2KIA9.
PRIDEiQ2KIA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000031050; ENSBTAP00000031013; ENSBTAG00000007000.
GeneIDi615106.
KEGGibta:615106.

Organism-specific databases

CTDi55556.

Phylogenomic databases

eggNOGiENOG410IET8. Eukaryota.
COG4948. LUCA.
GeneTreeiENSGT00390000014290.
HOGENOMiHOG000113757.
HOVERGENiHBG053904.
InParanoidiQ2KIA9.
KOiK18334.
OMAiSAAYCIL.
OrthoDBiEOG77DJ5S.
TreeFamiTF300529.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 2 hits.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiPS00909. MR_MLE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiENOF1_BOVIN
AccessioniPrimary (citable) accession number: Q2KIA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 7, 2006
Last modified: June 8, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.