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Protein

Mitochondrial enolase superfamily member 1

Gene

ENOSF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. May down-regulate thymidylate synthase activity, possibly already at the RNA level, by promoting the degradation of TYMS mRNA via an antisense RNA-based mechanism.By similarity

Catalytic activityi

L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34SubstrateBy similarity1
Binding sitei220SubstrateBy similarity1
Active sitei222Proton donor/acceptorBy similarity1
Metal bindingi250MagnesiumBy similarity1
Binding sitei252SubstrateBy similarity1
Metal bindingi276MagnesiumBy similarity1
Binding sitei276SubstrateBy similarity1
Metal bindingi305MagnesiumBy similarity1
Binding sitei305SubstrateBy similarity1
Active sitei355Sequence analysis1
Binding sitei386SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial enolase superfamily member 1 (EC:4.2.1.68By similarity)
Alternative name(s):
L-fuconate dehydratase
Gene namesi
Name:ENOSF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003316511 – 443Mitochondrial enolase superfamily member 1Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei148PhosphoserineBy similarity1

Post-translational modificationi

Could be sumoylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ2KIA9.
PRIDEiQ2KIA9.

Expressioni

Gene expression databases

BgeeiENSBTAG00000007000.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000031013.

Structurei

3D structure databases

ProteinModelPortaliQ2KIA9.
SMRiQ2KIA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate bindingBy similarity3
Regioni355 – 357Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IET8. Eukaryota.
COG4948. LUCA.
GeneTreeiENSGT00390000014290.
HOGENOMiHOG000113757.
HOVERGENiHBG053904.
InParanoidiQ2KIA9.
KOiK18334.
OMAiLVSCIDF.
OrthoDBiEOG091G027L.
TreeFamiTF300529.

Family and domain databases

CDDicd03324. rTSbeta_L-fuconate_dehydratase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiView protein in InterPro
IPR036849. Enolase-like_C.
IPR029017. Enolase-like_N.
IPR034390. Enolase-like_superfamily.
IPR029065. Enolase_C-like.
IPR034610. L-fuconate_dehydratase.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR034593. Mandelate_racemase-like.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
PANTHERiPTHR13794:SF58. PTHR13794:SF58. 1 hit.
PfamiView protein in Pfam
PF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
SFLDiSFLDF00111. L-fuconate_dehydratase. 1 hit.
SFLDG00179. mandelate_racemase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM00922. MR_MLE. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiView protein in PROSITE
PS00909. MR_MLE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2KIA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHGRVSRLS VHDVRFPTSL GGHGSDAMHT DPDYSAAYVV LETDAEDGLK
60 70 80 90 100
GYGITFTLGR GTEVVVCAVN ALAPHVLNKD LGEIVGDFRG FYRQLTSDGQ
110 120 130 140 150
LRWIGPEKGV VHLATAAVLN AVWDLWAKQE GKPLWKLLVD MDPRTLVSCI
160 170 180 190 200
DFRYITDVLT EEEACEILRQ SQVGKKEREE QMLAHGYPAY TTSCAWLGYP
210 220 230 240 250
DATLKQLCSE ALKDGWTRFK VKVGADLQDD IRRCRLVRNM IGPEKTLMMD
260 270 280 290 300
ANQRWDVPEA VEWMTKLAEF KPLWIEEPTS PDDILGHAAI SKALAPLGIG
310 320 330 340 350
VATGEQCHNR VIFKQLLQAK ALKFLQIDSC RLGSVNENLS VLLMAKKFEI
360 370 380 390 400
PVCPHAGGVG LCELVQHLII FDFISVSASL QDRMCEYVDH LHEHFKYPVL
410 420 430 440
IREAAYMPPK DAGYSTEMKE DSVKRHRYPD GEVWKKLLSA QGN
Length:443
Mass (Da):49,621
Last modified:March 7, 2006 - v1
Checksum:iC3D921D57C410AE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112706 mRNA. Translation: AAI12707.1.
RefSeqiNP_001040015.1. NM_001046550.2.
UniGeneiBt.59052.

Genome annotation databases

EnsembliENSBTAT00000031050; ENSBTAP00000031013; ENSBTAG00000007000.
GeneIDi615106.
KEGGibta:615106.

Similar proteinsi

Entry informationi

Entry nameiENOF1_BOVIN
AccessioniPrimary (citable) accession number: Q2KIA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 7, 2006
Last modified: November 22, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families