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Protein

Stearoyl-CoA desaturase 5

Gene

SCD5

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Stearyl-CoA desaturase that utilizes O2 and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids.By similarity

Catalytic activityi

Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.By similarity

Cofactori

Fe2+By similarityNote: Expected to bind 2 Fe2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541SubstrateBy similarity
Metal bindingi99 – 991Iron 1By similarity
Metal bindingi104 – 1041Iron 1By similarity
Binding sitei127 – 1271SubstrateBy similarity
Binding sitei134 – 1341SubstrateBy similarity
Binding sitei135 – 1351SubstrateBy similarity
Metal bindingi136 – 1361Iron 1By similarity
Metal bindingi139 – 1391Iron 2By similarity
Metal bindingi140 – 1401Iron 1By similarity
Binding sitei167 – 1671SubstrateBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Binding sitei241 – 2411SubstrateBy similarity
Metal bindingi248 – 2481Iron 2By similarity
Metal bindingi277 – 2771Iron 2By similarity
Metal bindingi280 – 2801Iron 1By similarity
Metal bindingi281 – 2811Iron 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.19.1. 908.

Names & Taxonomyi

Protein namesi
Recommended name:
Stearoyl-CoA desaturase 5 (EC:1.14.19.1By similarity)
Alternative name(s):
Acyl-CoA-desaturase 4
Stearoyl-CoA 9-desaturase
Gene namesi
Name:SCD5
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 6

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5454CytoplasmicCuratedAdd
BLAST
Transmembranei55 – 7521HelicalSequence analysisAdd
BLAST
Topological domaini76 – 772LumenalCurated
Transmembranei78 – 9821HelicalSequence analysisAdd
BLAST
Topological domaini99 – 198100CytoplasmicCuratedAdd
BLAST
Transmembranei199 – 21921HelicalSequence analysisAdd
BLAST
Topological domaini220 – 2278LumenalCurated
Transmembranei228 – 24720HelicalSequence analysisAdd
BLAST
Topological domaini248 – 33588CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Stearoyl-CoA desaturase 5PRO_0000312652Add
BLAST

Proteomic databases

PaxDbiQ2KIA4.
PRIDEiQ2KIA4.

Expressioni

Tissue specificityi

Detected in brain.1 Publication

Interactioni

Subunit structurei

May self-associate and form homodimers.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025820.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi99 – 1046Histidine box-1Curated
Motifi136 – 1405Histidine box-2Curated
Motifi277 – 2815Histidine box-3Curated

Domaini

The histidine box domains are involved in binding the catalytic metal ions.By similarity

Sequence similaritiesi

Belongs to the fatty acid desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1600. Eukaryota.
COG1398. LUCA.
GeneTreeiENSGT00530000063158.
HOGENOMiHOG000270352.
HOVERGENiHBG003367.
InParanoidiQ2KIA4.
KOiK00507.
OMAiMTPNIIG.
OrthoDBiEOG7ZPNKS.
TreeFamiTF313251.

Family and domain databases

InterProiIPR001522. Fatty_acid_desaturase-1_C.
IPR015876. Fatty_acid_desaturase-1_core.
IPR005804. Fatty_acid_desaturase_dom.
[Graphical view]
PfamiPF00487. FA_desaturase. 1 hit.
[Graphical view]
PRINTSiPR00075. FACDDSATRASE.
PROSITEiPS00476. FATTY_ACID_DESATUR_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KIA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGPAVDAEK VPFRSAKEEI RAGVGVEGSE GGGGGGGRER PGARGHRQDI
60 70 80 90 100
VWRNVFLMSL LHLAAVYSLV LIPKAQPLTL LWAYFCFLLT ALGVTAGAHR
110 120 130 140 150
LWSHRSYKAK LPLRIFLAAA NSMAFQNDIF EWSRDHRVHH KYSETDADPH
160 170 180 190 200
NARRGFFFSH IGWLFVRKHR DVIEKGRKLD VTDLLADPVV RFQRKYYKIT
210 220 230 240 250
VVLMCFVVPT LVPWYIWGES LWNSYFLASI LRYTISLNVT WLVNSVAHMY
260 270 280 290 300
GNRPYDKHIS PRQNPLVTLG AIGEGFHNYH HTFPFDYSAS EFGLNFNPTT
310 320 330
WFIDFMCWLG LATDRKRATK QMIEARKART GDGSA
Length:335
Mass (Da):38,215
Last modified:March 7, 2006 - v1
Checksum:i32F8B2C60533AB31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF014951 mRNA. Translation: ABJ99757.1.
BC112711 mRNA. Translation: AAI12712.1.
RefSeqiNP_001070413.1. NM_001076945.1.
UniGeneiBt.13249.

Genome annotation databases

EnsembliENSBTAT00000025820; ENSBTAP00000025820; ENSBTAG00000022449.
GeneIDi617419.
KEGGibta:617419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF014951 mRNA. Translation: ABJ99757.1.
BC112711 mRNA. Translation: AAI12712.1.
RefSeqiNP_001070413.1. NM_001076945.1.
UniGeneiBt.13249.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025820.

Proteomic databases

PaxDbiQ2KIA4.
PRIDEiQ2KIA4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000025820; ENSBTAP00000025820; ENSBTAG00000022449.
GeneIDi617419.
KEGGibta:617419.

Organism-specific databases

CTDi79966.

Phylogenomic databases

eggNOGiKOG1600. Eukaryota.
COG1398. LUCA.
GeneTreeiENSGT00530000063158.
HOGENOMiHOG000270352.
HOVERGENiHBG003367.
InParanoidiQ2KIA4.
KOiK00507.
OMAiMTPNIIG.
OrthoDBiEOG7ZPNKS.
TreeFamiTF313251.

Enzyme and pathway databases

BRENDAi1.14.19.1. 908.

Miscellaneous databases

NextBioi20900654.

Family and domain databases

InterProiIPR001522. Fatty_acid_desaturase-1_C.
IPR015876. Fatty_acid_desaturase-1_core.
IPR005804. Fatty_acid_desaturase_dom.
[Graphical view]
PfamiPF00487. FA_desaturase. 1 hit.
[Graphical view]
PRINTSiPR00075. FACDDSATRASE.
PROSITEiPS00476. FATTY_ACID_DESATUR_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel bovine stearoyl-CoA desaturase isoform with homology to human SCD5."
    Lengi A.J., Corl B.A.
    Lipids 42:499-508(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiSCD5_BOVIN
AccessioniPrimary (citable) accession number: Q2KIA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: March 7, 2006
Last modified: November 11, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.