Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histidine--tRNA ligase, cytoplasmic

Gene

HARS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cytoplasmic histidine--tRNA ligase. Plays a role in axon guidance.By similarity

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase, cytoplasmic (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:HARS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 509508Histidine--tRNA ligase, cytoplasmicPRO_0000245021Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei356 – 3561PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ2KI84.
PRIDEiQ2KI84.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025094.

Structurei

3D structure databases

ProteinModelPortaliQ2KI84.
SMRiQ2KI84. Positions 1-60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5957WHEP-TRSAdd
BLAST

Sequence similaritiesi

Contains 1 WHEP-TRS domain.Curated

Phylogenomic databases

eggNOGiKOG1936. Eukaryota.
COG0124. LUCA.
GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
HOVERGENiHBG002731.
InParanoidiQ2KI84.
KOiK01892.
OMAiIACMHEE.
OrthoDBiEOG7WMCJC.
TreeFamiTF300652.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00127. His_tRNA_synth.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR009068. S15_NS1_RNA-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KI84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRAALEDL VRVQGERVRG LKQQKASAEQ IEEEVAKLLK LKAQLGPDEG
60 70 80 90 100
KPKFVLKTPK GTRDYSPRQM AVREKVFDVI ISCFKRHGAE VIDTPVFELK
110 120 130 140 150
ETLTGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY
160 170 180 190 200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG QFDPMLPDAE CLKIMCEILS
210 220 230 240 250
SLQIGDFLVK VNDRRILDGM FAICGVPDSK FRTICSSVDK LDKVSWEEVK
260 270 280 290 300
NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG
310 320 330 340 350
DLKLLFEYLT LFGIADKISF DLSLARGLDY YTGVIYEAVL LQPPARAGEE
360 370 380 390 400
PLGVGSVAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL
410 420 430 440 450
EEKVRTTETQ VLVASAQKKL LEERLKLISE LWDAGIKAEL LYKKNPKLLN
460 470 480 490 500
QLQYCEETGI PLVAIIGEQE LKDGVIKLRS VASREEVDVR REDLVEEIKR

RTSQPLCIC
Length:509
Mass (Da):57,285
Last modified:March 7, 2006 - v1
Checksum:i54A2CAFD638D4358
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112731 mRNA. Translation: AAI12732.1.
RefSeqiNP_001039531.1. NM_001046066.2.
UniGeneiBt.49179.

Genome annotation databases

EnsembliENSBTAT00000025094; ENSBTAP00000025094; ENSBTAG00000018847.
GeneIDi510937.
KEGGibta:510937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112731 mRNA. Translation: AAI12732.1.
RefSeqiNP_001039531.1. NM_001046066.2.
UniGeneiBt.49179.

3D structure databases

ProteinModelPortaliQ2KI84.
SMRiQ2KI84. Positions 1-60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025094.

Proteomic databases

PaxDbiQ2KI84.
PRIDEiQ2KI84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000025094; ENSBTAP00000025094; ENSBTAG00000018847.
GeneIDi510937.
KEGGibta:510937.

Organism-specific databases

CTDi3035.

Phylogenomic databases

eggNOGiKOG1936. Eukaryota.
COG0124. LUCA.
GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
HOVERGENiHBG002731.
InParanoidiQ2KI84.
KOiK01892.
OMAiIACMHEE.
OrthoDBiEOG7WMCJC.
TreeFamiTF300652.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00127. His_tRNA_synth.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR009068. S15_NS1_RNA-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiSYHC_BOVIN
AccessioniPrimary (citable) accession number: Q2KI84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 7, 2006
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.