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Protein

Glucosylceramidase

Gene

GBA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei274 – 2741Proton donorBy similarity
Active sitei379 – 3791NucleophileBy similarity

GO - Molecular functioni

  1. glucosylceramidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. sphingolipid metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosylceramidase (EC:3.2.1.45)
Alternative name(s):
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Gene namesi
Name:GBA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

Lysosome membrane By similarity; Peripheral membrane protein By similarity
Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2.By similarity

GO - Cellular componenti

  1. lysosomal membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939By similarityAdd
BLAST
Chaini40 – 536497GlucosylceramidasePRO_0000278648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 55By similarity
Disulfide bondi57 ↔ 62By similarity
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ2KHZ8.

Interactioni

Subunit structurei

Interacts with SCARB2.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019765.

Structurei

3D structure databases

ProteinModelPortaliQ2KHZ8.
SMRiQ2KHZ8. Positions 40-536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5520.
HOGENOMiHOG000270031.
HOVERGENiHBG002285.
InParanoidiQ2KHZ8.
KOiK01201.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KHZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSSPSREE YPMPRGRVGI MAASLMGLLL LHTVSWVSGA RPCSPKSFGY
60 70 80 90 100
SSVVCVCNGT YCDSLDPLTL PDPGTFSRFE STRSGRRMEL SLGTIQANRT
110 120 130 140 150
GTGLLLTLQP DQKFQKVKGF GGAMTDAAAL NILALSPAAR NLLLKSYFSE
160 170 180 190 200
EGIEYNIIRV PMASCDFSIR TYTYDDSPDD FQLLNFSLPE EDVKLKIPLI
210 220 230 240 250
HQALELANRS VSLFASPWTS PTWLKTNGAV NGKGTLKGQA GDLYHKTWAR
260 270 280 290 300
YFVKFLDAYA EHKLRFWAVT AENEPTAGLL TGYPFQCLGF TPEHQRDFIA
310 320 330 340 350
RDLGPILANS THRDVRLLML DDQRLLLPRW AQVVLADPEA AKYVHGIAVH
360 370 380 390 400
WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM
410 420 430 440 450
RYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDIAKDTF
460 470 480 490 500
YKQPMFYHLG HFSKFIPEGS QRVGLVASKK SDLDTVALLR PDGSAVAVVL
510 520 530
NRSSKDVPLT IKDPAVGFME TVSPGYSIHT YLWRRQ
Length:536
Mass (Da):59,855
Last modified:March 7, 2006 - v1
Checksum:i1260A9C428E5D3FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112823 mRNA. Translation: AAI12824.1.
RefSeqiNP_001039886.1. NM_001046421.2.
UniGeneiBt.5413.

Genome annotation databases

GeneIDi537087.
KEGGibta:537087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112823 mRNA. Translation: AAI12824.1.
RefSeqiNP_001039886.1. NM_001046421.2.
UniGeneiBt.5413.

3D structure databases

ProteinModelPortaliQ2KHZ8.
SMRiQ2KHZ8. Positions 40-536.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019765.

Chemistry

BindingDBiQ2KHZ8.
ChEMBLiCHEMBL1275219.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Proteomic databases

PRIDEiQ2KHZ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi537087.
KEGGibta:537087.

Organism-specific databases

CTDi2629.

Phylogenomic databases

eggNOGiCOG5520.
HOGENOMiHOG000270031.
HOVERGENiHBG002285.
InParanoidiQ2KHZ8.
KOiK01201.

Miscellaneous databases

NextBioi20877067.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Heart ventricle.

Entry informationi

Entry nameiGLCM_BOVIN
AccessioniPrimary (citable) accession number: Q2KHZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 7, 2006
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.