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Q2KHZ8

- GLCM_BOVIN

UniProt

Q2KHZ8 - GLCM_BOVIN

Protein

Glucosylceramidase

Gene

GBA

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei274 – 2741Proton donorBy similarity
    Active sitei379 – 3791NucleophileBy similarity

    GO - Molecular functioni

    1. glucosylceramidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. sphingolipid metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Protein family/group databases

    CAZyiGH30. Glycoside Hydrolase Family 30.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosylceramidase (EC:3.2.1.45)
    Alternative name(s):
    Acid beta-glucosidase
    Beta-glucocerebrosidase
    D-glucosyl-N-acylsphingosine glucohydrolase
    Gene namesi
    Name:GBA
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Lysosome membrane By similarity; Peripheral membrane protein By similarity
    Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2.By similarity

    GO - Cellular componenti

    1. lysosomal membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3939By similarityAdd
    BLAST
    Chaini40 – 536497GlucosylceramidasePRO_0000278648Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 55By similarity
    Disulfide bondi57 ↔ 62By similarity
    Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ2KHZ8.

    Interactioni

    Subunit structurei

    Interacts with SCARB2.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000019765.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2KHZ8.
    SMRiQ2KHZ8. Positions 40-536.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 30 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5520.
    HOGENOMiHOG000270031.
    HOVERGENiHBG002285.
    InParanoidiQ2KHZ8.
    KOiK01201.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    PRINTSiPR00843. GLHYDRLASE30.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2KHZ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELSSPSREE YPMPRGRVGI MAASLMGLLL LHTVSWVSGA RPCSPKSFGY    50
    SSVVCVCNGT YCDSLDPLTL PDPGTFSRFE STRSGRRMEL SLGTIQANRT 100
    GTGLLLTLQP DQKFQKVKGF GGAMTDAAAL NILALSPAAR NLLLKSYFSE 150
    EGIEYNIIRV PMASCDFSIR TYTYDDSPDD FQLLNFSLPE EDVKLKIPLI 200
    HQALELANRS VSLFASPWTS PTWLKTNGAV NGKGTLKGQA GDLYHKTWAR 250
    YFVKFLDAYA EHKLRFWAVT AENEPTAGLL TGYPFQCLGF TPEHQRDFIA 300
    RDLGPILANS THRDVRLLML DDQRLLLPRW AQVVLADPEA AKYVHGIAVH 350
    WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM 400
    RYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDIAKDTF 450
    YKQPMFYHLG HFSKFIPEGS QRVGLVASKK SDLDTVALLR PDGSAVAVVL 500
    NRSSKDVPLT IKDPAVGFME TVSPGYSIHT YLWRRQ 536
    Length:536
    Mass (Da):59,855
    Last modified:March 7, 2006 - v1
    Checksum:i1260A9C428E5D3FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC112823 mRNA. Translation: AAI12824.1.
    RefSeqiNP_001039886.1. NM_001046421.2.
    UniGeneiBt.5413.

    Genome annotation databases

    GeneIDi537087.
    KEGGibta:537087.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC112823 mRNA. Translation: AAI12824.1 .
    RefSeqi NP_001039886.1. NM_001046421.2.
    UniGenei Bt.5413.

    3D structure databases

    ProteinModelPortali Q2KHZ8.
    SMRi Q2KHZ8. Positions 40-536.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000019765.

    Chemistry

    BindingDBi Q2KHZ8.
    ChEMBLi CHEMBL1275219.

    Protein family/group databases

    CAZyi GH30. Glycoside Hydrolase Family 30.

    Proteomic databases

    PRIDEi Q2KHZ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 537087.
    KEGGi bta:537087.

    Organism-specific databases

    CTDi 2629.

    Phylogenomic databases

    eggNOGi COG5520.
    HOGENOMi HOG000270031.
    HOVERGENi HBG002285.
    InParanoidi Q2KHZ8.
    KOi K01201.

    Miscellaneous databases

    NextBioi 20877067.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR11069. PTHR11069. 1 hit.
    Pfami PF02055. Glyco_hydro_30. 1 hit.
    [Graphical view ]
    PRINTSi PR00843. GLHYDRLASE30.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Heart ventricle.

    Entry informationi

    Entry nameiGLCM_BOVIN
    AccessioniPrimary (citable) accession number: Q2KHZ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3