Reviewed, UniProtKB/Swiss-Prot Q2KHZ8 (GLCM_BOVIN)

Names and origin

Protein names

Recommended name:
    Glucosylceramidase
    EC=3.2.1.45
Alternative name(s):
    Beta-glucocerebrosidase
    Acid beta-glucosidase
    D-glucosyl-N-acylsphingosine glucohydrolase

Gene names

Name:

GBA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.
Subcellular location	Lysosome membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 30 family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Lysosome Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lysosome organization Inferred from electronic annotation. Source: InterPro sphingolipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucosylceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	By similarity
	Chain	40 – 536	497	Glucosylceramidase		PRO_0000278648
Sites
	Active site	274	1	Proton donor By similarity
	Active site	379	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Glycosylation	208	1	N-linked (GlcNAc...) Potential
	Glycosylation	309	1	N-linked (GlcNAc...) Potential
	Glycosylation	501	1	N-linked (GlcNAc...) Potential
	Disulfide bond	43 ↔ 55		By similarity
	Disulfide bond	57 ↔ 62		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KHZ8-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 1260A9C428E5D3FE	FASTA	536	59,855
	10 20 30 40 50 60 MELSSPSREE YPMPRGRVGI MAASLMGLLL LHTVSWVSGA RPCSPKSFGY SSVVCVCNGT 70 80 90 100 110 120 YCDSLDPLTL PDPGTFSRFE STRSGRRMEL SLGTIQANRT GTGLLLTLQP DQKFQKVKGF 130 140 150 160 170 180 GGAMTDAAAL NILALSPAAR NLLLKSYFSE EGIEYNIIRV PMASCDFSIR TYTYDDSPDD 190 200 210 220 230 240 FQLLNFSLPE EDVKLKIPLI HQALELANRS VSLFASPWTS PTWLKTNGAV NGKGTLKGQA 250 260 270 280 290 300 GDLYHKTWAR YFVKFLDAYA EHKLRFWAVT AENEPTAGLL TGYPFQCLGF TPEHQRDFIA 310 320 330 340 350 360 RDLGPILANS THRDVRLLML DDQRLLLPRW AQVVLADPEA AKYVHGIAVH WYLDFLAPAK 370 380 390 400 410 420 ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM RYSHSIITNL LYHVVGWTDW 430 440 450 460 470 480 NLALNPEGGP NWVRNFVDSP IIVDIAKDTF YKQPMFYHLG HFSKFIPEGS QRVGLVASKK 490 500 510 520 530 SDLDTVALLR PDGSAVAVVL NRSSKDVPLT IKDPAVGFME TVSPGYSIHT YLWRRQ

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC112823 mRNA. Translation: AAI12824.1.
IPI	IPI00717765.
RefSeq	NP_001039886.1.
UniGene	Bt.5413
3D structure databases
SMR	Q2KHZ8. Positions 40-536.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KHZ8.
Protein family/group databases
CAZy	GH30. Glycoside Hydrolase Family 30.
Genome annotation databases
Ensembl	ENSBTAT00000019765; ENSBTAP00000019765; ENSBTAG00000014841; Bos taurus. [Genome view]
GeneID	537087.
KEGG	bta:537087.
Organism-specific databases
CTD	537087.
Phylogenomic databases
HOVERGEN	Q2KHZ8.
Enzyme and pathway databases
BRENDA	3.2.1.45. 251.
Family and domain databases
InterPro	IPR001139. Glyco_hydro_30. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR11069. Glyco_hydro_30. 1 hit.
Pfam	PF02055. Glyco_hydro_30. 1 hit. [Graphical view]
PRINTS	PR00843. GLHYDRLASE30.
ProtoNet	Search...

Entry information

Entry name

GLCM_BOVIN

Accession

Primary (citable) accession number: Q2KHZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	March 7, 2006
Last modified:	October 13, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)