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Q2KHZ8 (GLCM_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucosylceramidase

EC=3.2.1.45
Alternative name(s):
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Gene names
Name:GBA
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length536 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Subunit structure

Interacts with SCARB2 By similarity.

Subcellular location

Lysosome membrane; Peripheral membrane protein By similarity. Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Ontologies

Keywords
   Biological processLipid metabolism
Sphingolipid metabolism
   Cellular componentLysosome
Membrane
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

sphingolipid metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentlysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucosylceramidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 By similarity
Chain40 – 536497Glucosylceramidase
PRO_0000278648

Sites

Active site2741Proton donor By similarity
Active site3791Nucleophile By similarity

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1851N-linked (GlcNAc...) Potential
Glycosylation2081N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation5011N-linked (GlcNAc...) Potential
Disulfide bond43 ↔ 55 By similarity
Disulfide bond57 ↔ 62 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2KHZ8 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 1260A9C428E5D3FE

FASTA53659,855
        10         20         30         40         50         60 
MELSSPSREE YPMPRGRVGI MAASLMGLLL LHTVSWVSGA RPCSPKSFGY SSVVCVCNGT 

        70         80         90        100        110        120 
YCDSLDPLTL PDPGTFSRFE STRSGRRMEL SLGTIQANRT GTGLLLTLQP DQKFQKVKGF 

       130        140        150        160        170        180 
GGAMTDAAAL NILALSPAAR NLLLKSYFSE EGIEYNIIRV PMASCDFSIR TYTYDDSPDD 

       190        200        210        220        230        240 
FQLLNFSLPE EDVKLKIPLI HQALELANRS VSLFASPWTS PTWLKTNGAV NGKGTLKGQA 

       250        260        270        280        290        300 
GDLYHKTWAR YFVKFLDAYA EHKLRFWAVT AENEPTAGLL TGYPFQCLGF TPEHQRDFIA 

       310        320        330        340        350        360 
RDLGPILANS THRDVRLLML DDQRLLLPRW AQVVLADPEA AKYVHGIAVH WYLDFLAPAK 

       370        380        390        400        410        420 
ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM RYSHSIITNL LYHVVGWTDW 

       430        440        450        460        470        480 
NLALNPEGGP NWVRNFVDSP IIVDIAKDTF YKQPMFYHLG HFSKFIPEGS QRVGLVASKK 

       490        500        510        520        530 
SDLDTVALLR PDGSAVAVVL NRSSKDVPLT IKDPAVGFME TVSPGYSIHT YLWRRQ 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Heart ventricle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC112823 mRNA. Translation: AAI12824.1.
RefSeqNP_001039886.1. NM_001046421.2.
UniGeneBt.5413.

3D structure databases

ProteinModelPortalQ2KHZ8.
SMRQ2KHZ8. Positions 40-536.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000019765.

Chemistry

BindingDBQ2KHZ8.
ChEMBLCHEMBL1275219.

Protein family/group databases

CAZyGH30. Glycoside Hydrolase Family 30.

Proteomic databases

PRIDEQ2KHZ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID537087.
KEGGbta:537087.

Organism-specific databases

CTD2629.

Phylogenomic databases

eggNOGCOG5520.
HOGENOMHOG000270031.
HOVERGENHBG002285.
InParanoidQ2KHZ8.
KOK01201.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR11069. PTHR11069. 1 hit.
PfamPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSPR00843. GLHYDRLASE30.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

NextBio20877067.

Entry information

Entry nameGLCM_BOVIN
AccessionPrimary (citable) accession number: Q2KHZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 7, 2006
Last modified: January 22, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries