Reviewed, UniProtKB/Swiss-Prot Q2KHW5 (ACBG1_BOVIN)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--CoA ligase ACSBG1
    EC=6.2.1.3
Alternative name(s):
    Acyl-CoA synthetase bubblegum family member 1

Gene names

Name:

ACSBG1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Subcellular location	Cytoplasm By similarity. Cytoplasmic vesicle By similarity. Microsome By similarity. Endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.
Sequence caution	The sequence AAX46711.1 differs from that shown. Reason: Frameshift at position 705.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Cytoplasm Cytoplasmic vesicle Endoplasmic reticulum Microsome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	Long-chain-fatty-acid--CoA ligase ACSBG1		PRO_0000315807
Amino acid modifications
	Modified residue	118	1	Phosphotyrosine By similarity
	Modified residue	121	1	Phosphotyrosine By similarity
	Modified residue	122	1	Phosphotyrosine By similarity
	Modified residue	641	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KHW5-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 22EC0E08E143FB72	FASTA	726	80,585
	10 20 30 40 50 60 MPDSRAAPQE SLLDASLGTT QENVGTSSLT DGQTLSKEPL SHALKLSTPE KVKDAQLNPP 70 80 90 100 110 120 EEALWTTRAD GRVRLRIDPI CSQTPRTVHQ MFSTTLDKYG DLSAMGFKRQ GTWEHISYTQ 130 140 150 160 170 180 YYLLARKAAK GFLKLGLERA HSVAILAFNS PEWFFSAVGA VFGGGIITGI YTTSSPEACQ 190 200 210 220 230 240 YIAYDCRANI IVVDTQKQLE KILKIWKHLP HLKAVVIYRE APPMRMPSVY TMEELMELGN 250 260 270 280 290 300 EVPEEALDVI INAQKPNQCC ALVYTSGTTG NPKGVMLSQD NITWTARYGS QAGDIQPAEI 310 320 330 340 350 360 QQEVVVSYLP LSHIAAQIYD LWTGIQWGAQ VCFAEPDALK GSLVNTLREV EPTSHMGVPR 370 380 390 400 410 420 VWEKIMEQIQ EVAAQSGFIW RKMLLWAMSV TLEQNLTCPS SDLKPFTTRL ADYLVLAKVR 430 440 450 460 470 480 QALGFAKCQK NFYGAAPMTA ETQHFFLGLN IRLYAGYGLS ETSGPHFMSS PYNYRLYSSG 490 500 510 520 530 540 KVVPGCQVKL VNEDAEGIGE ICLWGRTIFM GYLNMEDKTC EAIDAEGWLH TGDTGRLDAD 550 560 570 580 590 600 GFLYITGRLK ELIITAGGEN VPPVPIEEAV KTELPIIRNA MLIGDQRKFL SMLLTLKCTL 610 620 630 640 650 660 DPDTFEPTDN LTEQAVEFCQ RVGSKATTVS EVVGKKDEAV YQAIEEGIQR VNMNAAARPY 670 680 690 700 710 720 HIQKWAILEK DFSISGGELG PTMKLKRLAV LEKYKDVIDS FYQEQKSKQG SSLPGFSLRW QTGASS

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT021864 mRNA. Translation: AAX46711.1. Frameshift. BC112858 mRNA. Translation: AAI12859.1.
IPI	IPI00710534.
RefSeq	NP_001019719.1.
UniGene	Bt.37833
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000006231. Bos taurus. [Contig view]
GeneID	515577.
KEGG	bta:515577.
Phylogenomic databases
HOVERGEN	Q2KHW5.
OMA	Q2KHW5. HMGVPRV.
Enzyme and pathway databases
BRENDA	6.2.1.3. 251.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACBG1_BOVIN

Accession

Primary (citable) accession number: Q2KHW5
Secondary accession number(s): Q58CT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)