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Q2KHV7

- UBP2_BOVIN

UniProt

Q2KHV7 - UBP2_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

USP2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Possesses both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Cofactori

    Binds 1 zinc ion.By similarity

    Enzyme regulationi

    Cleavage is inhibited by ubiquitin in a dosage-dependent manner. Cleavage is blocked by ubiquitin aldehyde By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei277 – 2771NucleophilePROSITE-ProRule annotation
    Metal bindingi426 – 4261ZincBy similarity
    Metal bindingi429 – 4291ZincBy similarity
    Metal bindingi477 – 4771ZincBy similarity
    Metal bindingi480 – 4801ZincBy similarity
    Active sitei558 – 5581Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. muscle organ development Source: UniProtKB-KW
    3. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    4. positive regulation of mitotic cell cycle Source: UniProtKB
    5. protein deubiquitination Source: UniProtKB
    6. protein stabilization Source: UniProtKB
    7. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, Myogenesis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.013.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
    Alternative name(s):
    41 kDa ubiquitin-specific protease
    Deubiquitinating enzyme 2
    Ubiquitin thioesterase 2
    Ubiquitin-specific-processing protease 2
    Gene namesi
    Name:USP2
    Synonyms:UBP41
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 15

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmperinuclear region By similarity
    Note: Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane.By similarity

    GO - Cellular componenti

    1. cell cortex Source: Ensembl
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 606606Ubiquitin carboxyl-terminal hydrolase 2PRO_0000395965Add
    BLAST

    Proteomic databases

    PRIDEiQ2KHV7.

    Interactioni

    Subunit structurei

    Homooligomer By similarity. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C-terminus) with MDM2. Interacts with MDM4 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000012857.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2KHV7.
    SMRiQ2KHV7. Positions 264-601.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini268 – 600333USPAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 201201Necessary for interaction with MDM4By similarityAdd
    BLAST
    Regioni404 – 504101Necessary for interaction with MDM4By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP2 subfamily.Curated
    Contains 1 USP domain.Curated

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5533.
    GeneTreeiENSGT00750000117710.
    HOGENOMiHOG000048720.
    HOVERGENiHBG011164.
    InParanoidiQ2KHV7.
    KOiK11833.
    OMAiKSKGQAP.
    OrthoDBiEOG7FR7GN.
    TreeFamiTF106277.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2KHV7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQLSSTLKR YTESARFTDA PFTKSSYGTY TPSSYGTNLA ASFLEKEKFG    50
    FKPSPPTSYL TRPRTYGPPS ILDYDRGRPL LRPDVIGGGK RAESQTRGTE 100
    RPSGSGLSGG SGFSYGVTTS SVSYLPVSAR DQGVTLTQKK SNSQSDLARD 150
    FSSLQTSDSY RLDSGNLGRS PMLARTRKEL CALQGLYQAA SRSEYLADYL 200
    ENYGRKASAP QVPTPTPPSR APEVLSPTYR PSGRYSLWEK GKGQALVSSR 250
    SSSPGRDTMN SKSAQGLAGL RNLGNTCFMN SILQCLSNTR ELRDYCLQRL 300
    YLRDLSHSSR AHTALMEEFA KLIQTIWTSS PNDVVSPSEF KTQIQRYAPR 350
    FVGYNQQDAQ EFLRFLLDGL HNEVNRVIAR PKSNTENLDH LPDDEKGRQM 400
    WRKYLEREDS RIGDLFVGQL KSSLTCTDCG YCSTVFDPFW DLSLPITKRG 450
    YPEVTLMDCM RLFTKEDVLD GDEKPTCCRC RARKRCIKKF SIQRFPKILV 500
    LHLKRFSESR IRTSKLTAFV NFPLRDLDLR EFASENTNHA VYNLYAVSNH 550
    SGTTMGGHYT AYCRSPVTGE WHTFNDSSVS PMSSSQVRTS DAYLLFYELA 600
    SPPSRM 606
    Length:606
    Mass (Da):68,101
    Last modified:March 7, 2006 - v1
    Checksum:i4713919E8BDEE909
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC112866 mRNA. Translation: AAI12867.1.
    RefSeqiNP_001039728.1. NM_001046263.1.
    XP_005216024.1. XM_005215967.1.
    UniGeneiBt.21721.

    Genome annotation databases

    EnsembliENSBTAT00000012857; ENSBTAP00000012857; ENSBTAG00000009749.
    GeneIDi522980.
    KEGGibta:522980.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC112866 mRNA. Translation: AAI12867.1 .
    RefSeqi NP_001039728.1. NM_001046263.1.
    XP_005216024.1. XM_005215967.1.
    UniGenei Bt.21721.

    3D structure databases

    ProteinModelPortali Q2KHV7.
    SMRi Q2KHV7. Positions 264-601.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000012857.

    Protein family/group databases

    MEROPSi C19.013.

    Proteomic databases

    PRIDEi Q2KHV7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000012857 ; ENSBTAP00000012857 ; ENSBTAG00000009749 .
    GeneIDi 522980.
    KEGGi bta:522980.

    Organism-specific databases

    CTDi 9099.

    Phylogenomic databases

    eggNOGi COG5533.
    GeneTreei ENSGT00750000117710.
    HOGENOMi HOG000048720.
    HOVERGENi HBG011164.
    InParanoidi Q2KHV7.
    KOi K11833.
    OMAi KSKGQAP.
    OrthoDBi EOG7FR7GN.
    TreeFami TF106277.

    Miscellaneous databases

    NextBioi 20873641.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Heart ventricle.

    Entry informationi

    Entry nameiUBP2_BOVIN
    AccessioniPrimary (citable) accession number: Q2KHV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3