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Q2KHV7 (UBP2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 2
EC=3.4.19.12
Alternative name(s):
41 kDa ubiquitin-specific protease
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene names
Name:USP2
Synonyms:UBP41
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Possesses both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Cofactor

Binds 1 zinc ion By similarity.

Enzyme regulation

Cleavage is inhibited by ubiquitin in a dosage-dependent manner. Cleavage is blocked by ubiquitin aldehyde By similarity.

Subunit structure

Homooligomer By similarity. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C-terminus) with MDM2. Interacts with MDM4 By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasm › perinuclear region By similarity. Note: Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane By similarity.

Sequence similarities

Belongs to the peptidase C19 family. USP2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606Ubiquitin carboxyl-terminal hydrolase 2
PRO_0000395965

Regions

Region1 – 201201Necessary for interaction with MDM4 By similarity
Region404 – 504101Necessary for interaction with MDM4 By similarity

Sites

Active site2771Nucleophile By similarity
Active site5581Proton acceptor By similarity
Metal binding4261Zinc By similarity
Metal binding4291Zinc By similarity
Metal binding4771Zinc By similarity
Metal binding4801Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2KHV7 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 4713919E8BDEE909

FASTA60668,101
        10         20         30         40         50         60 
MSQLSSTLKR YTESARFTDA PFTKSSYGTY TPSSYGTNLA ASFLEKEKFG FKPSPPTSYL 

        70         80         90        100        110        120 
TRPRTYGPPS ILDYDRGRPL LRPDVIGGGK RAESQTRGTE RPSGSGLSGG SGFSYGVTTS 

       130        140        150        160        170        180 
SVSYLPVSAR DQGVTLTQKK SNSQSDLARD FSSLQTSDSY RLDSGNLGRS PMLARTRKEL 

       190        200        210        220        230        240 
CALQGLYQAA SRSEYLADYL ENYGRKASAP QVPTPTPPSR APEVLSPTYR PSGRYSLWEK 

       250        260        270        280        290        300 
GKGQALVSSR SSSPGRDTMN SKSAQGLAGL RNLGNTCFMN SILQCLSNTR ELRDYCLQRL 

       310        320        330        340        350        360 
YLRDLSHSSR AHTALMEEFA KLIQTIWTSS PNDVVSPSEF KTQIQRYAPR FVGYNQQDAQ 

       370        380        390        400        410        420 
EFLRFLLDGL HNEVNRVIAR PKSNTENLDH LPDDEKGRQM WRKYLEREDS RIGDLFVGQL 

       430        440        450        460        470        480 
KSSLTCTDCG YCSTVFDPFW DLSLPITKRG YPEVTLMDCM RLFTKEDVLD GDEKPTCCRC 

       490        500        510        520        530        540 
RARKRCIKKF SIQRFPKILV LHLKRFSESR IRTSKLTAFV NFPLRDLDLR EFASENTNHA 

       550        560        570        580        590        600 
VYNLYAVSNH SGTTMGGHYT AYCRSPVTGE WHTFNDSSVS PMSSSQVRTS DAYLLFYELA 


SPPSRM

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Heart ventricle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC112866 mRNA. Translation: AAI12867.1.
IPIIPI00694687.
RefSeqNP_001039728.1. NM_001046263.1.
UniGeneBt.21721.

3D structure databases

ProteinModelPortalQ2KHV7.
SMRQ2KHV7. Positions 264-601.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000012857.

Protein family/group databases

MEROPSC19.013.

Proteomic databases

PRIDEQ2KHV7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000012857; ENSBTAP00000012857; ENSBTAG00000009749.
GeneID522980.
KEGGbta:522980.

Organism-specific databases

CTD9099.

Phylogenomic databases

eggNOGCOG5533.
GeneTreeENSGT00670000098024.
HOGENOMHOG000048720.
HOVERGENHBG011164.
InParanoidQ2KHV7.
KOK11833.
OMAGFKPGPP.
OrthoDBEOG47M1XH.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. 1 hit.
PS50235. UCH_2_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20873641.

Entry information

Entry nameUBP2_BOVIN
AccessionPrimary (citable) accession number: Q2KHV7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: March 7, 2006
Last modified: April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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