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Q2KHV7

- UBP2_BOVIN

UniProt

Q2KHV7 - UBP2_BOVIN

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Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

USP2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Possesses both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Enzyme regulationi

Cleavage is inhibited by ubiquitin in a dosage-dependent manner. Cleavage is blocked by ubiquitin aldehyde (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei277 – 2771NucleophilePROSITE-ProRule annotation
Metal bindingi426 – 4261ZincBy similarity
Metal bindingi429 – 4291ZincBy similarity
Metal bindingi477 – 4771ZincBy similarity
Metal bindingi480 – 4801ZincBy similarity
Active sitei558 – 5581Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: Ensembl
  3. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. muscle organ development Source: UniProtKB-KW
  3. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  4. positive regulation of mitotic cell cycle Source: UniProtKB
  5. protein deubiquitination Source: UniProtKB
  6. protein stabilization Source: UniProtKB
  7. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Myogenesis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Alternative name(s):
41 kDa ubiquitin-specific protease
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene namesi
Name:USP2
Synonyms:UBP41
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 15

Subcellular locationi

Cytoplasm By similarity. Cytoplasmperinuclear region By similarity
Note: Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane.By similarity

GO - Cellular componenti

  1. cell cortex Source: Ensembl
  2. perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Ubiquitin carboxyl-terminal hydrolase 2PRO_0000395965Add
BLAST

Proteomic databases

PRIDEiQ2KHV7.

Interactioni

Subunit structurei

Homooligomer (By similarity). Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C-terminus) with MDM2. Interacts with MDM4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012857.

Structurei

3D structure databases

ProteinModelPortaliQ2KHV7.
SMRiQ2KHV7. Positions 264-601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini268 – 600333USPAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 201201Necessary for interaction with MDM4By similarityAdd
BLAST
Regioni404 – 504101Necessary for interaction with MDM4By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP2 subfamily.Curated
Contains 1 USP domain.Curated

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00760000119208.
HOGENOMiHOG000048720.
HOVERGENiHBG011164.
InParanoidiQ2KHV7.
KOiK11833.
OMAiKSKGQAP.
OrthoDBiEOG7FR7GN.
TreeFamiTF106277.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KHV7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQLSSTLKR YTESARFTDA PFTKSSYGTY TPSSYGTNLA ASFLEKEKFG
60 70 80 90 100
FKPSPPTSYL TRPRTYGPPS ILDYDRGRPL LRPDVIGGGK RAESQTRGTE
110 120 130 140 150
RPSGSGLSGG SGFSYGVTTS SVSYLPVSAR DQGVTLTQKK SNSQSDLARD
160 170 180 190 200
FSSLQTSDSY RLDSGNLGRS PMLARTRKEL CALQGLYQAA SRSEYLADYL
210 220 230 240 250
ENYGRKASAP QVPTPTPPSR APEVLSPTYR PSGRYSLWEK GKGQALVSSR
260 270 280 290 300
SSSPGRDTMN SKSAQGLAGL RNLGNTCFMN SILQCLSNTR ELRDYCLQRL
310 320 330 340 350
YLRDLSHSSR AHTALMEEFA KLIQTIWTSS PNDVVSPSEF KTQIQRYAPR
360 370 380 390 400
FVGYNQQDAQ EFLRFLLDGL HNEVNRVIAR PKSNTENLDH LPDDEKGRQM
410 420 430 440 450
WRKYLEREDS RIGDLFVGQL KSSLTCTDCG YCSTVFDPFW DLSLPITKRG
460 470 480 490 500
YPEVTLMDCM RLFTKEDVLD GDEKPTCCRC RARKRCIKKF SIQRFPKILV
510 520 530 540 550
LHLKRFSESR IRTSKLTAFV NFPLRDLDLR EFASENTNHA VYNLYAVSNH
560 570 580 590 600
SGTTMGGHYT AYCRSPVTGE WHTFNDSSVS PMSSSQVRTS DAYLLFYELA

SPPSRM
Length:606
Mass (Da):68,101
Last modified:March 7, 2006 - v1
Checksum:i4713919E8BDEE909
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112866 mRNA. Translation: AAI12867.1.
RefSeqiNP_001039728.1. NM_001046263.1.
XP_005216024.1. XM_005215967.1.
UniGeneiBt.21721.

Genome annotation databases

EnsembliENSBTAT00000012857; ENSBTAP00000012857; ENSBTAG00000009749.
GeneIDi522980.
KEGGibta:522980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112866 mRNA. Translation: AAI12867.1 .
RefSeqi NP_001039728.1. NM_001046263.1.
XP_005216024.1. XM_005215967.1.
UniGenei Bt.21721.

3D structure databases

ProteinModelPortali Q2KHV7.
SMRi Q2KHV7. Positions 264-601.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000012857.

Protein family/group databases

MEROPSi C19.013.

Proteomic databases

PRIDEi Q2KHV7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000012857 ; ENSBTAP00000012857 ; ENSBTAG00000009749 .
GeneIDi 522980.
KEGGi bta:522980.

Organism-specific databases

CTDi 9099.

Phylogenomic databases

eggNOGi COG5533.
GeneTreei ENSGT00760000119208.
HOGENOMi HOG000048720.
HOVERGENi HBG011164.
InParanoidi Q2KHV7.
KOi K11833.
OMAi KSKGQAP.
OrthoDBi EOG7FR7GN.
TreeFami TF106277.

Miscellaneous databases

NextBioi 20873641.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Heart ventricle.

Entry informationi

Entry nameiUBP2_BOVIN
AccessioniPrimary (citable) accession number: Q2KHV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: March 7, 2006
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3