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Protein

Presenilins-associated rhomboid-like protein, mitochondrial

Gene

PARL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity).By similarity

Catalytic activityi

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei275 – 2751NucleophileBy similarity
Active sitei333 – 3331By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilins-associated rhomboid-like protein, mitochondrial (EC:3.4.21.105)
Alternative name(s):
Mitochondrial intramembrane cleaving protease PARL
Cleaved into the following chain:
P-beta
Short name:
Pbeta
Gene namesi
Name:PARL
Synonyms:PSARL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Subcellular locationi

  • Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity
P-beta :
  • Nucleus By similarity

  • Note: Translocated into the nucleus by an unknown mechanism.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini53 – 9947Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei100 – 11920HelicalSequence analysisAdd
BLAST
Topological domaini120 – 16546Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei166 – 18520HelicalSequence analysisAdd
BLAST
Topological domaini186 – 20520Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei206 – 22823HelicalSequence analysisAdd
BLAST
Topological domaini229 – 24214Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei243 – 26018HelicalSequence analysisAdd
BLAST
Topological domaini261 – 27010Mitochondrial matrixSequence analysis
Transmembranei271 – 28717HelicalSequence analysisAdd
BLAST
Topological domaini288 – 2936Mitochondrial intermembraneSequence analysis
Transmembranei294 – 31623HelicalSequence analysisAdd
BLAST
Topological domaini317 – 33014Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei331 – 35222HelicalSequence analysisAdd
BLAST
Topological domaini353 – 37725Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252MitochondrionBy similarityAdd
BLAST
Chaini53 – 377325Presenilins-associated rhomboid-like protein, mitochondrialPRO_0000245093Add
BLAST
Peptidei53 – 7523P-betaBy similarityPRO_0000245094Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651PhosphoserineBy similarity
Modified residuei68 – 681PhosphoserineBy similarity

Post-translational modificationi

P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2KHV4.
PRIDEiQ2KHV4.

Interactioni

Subunit structurei

Interacts with PSEN1 and PSEN2. Binds OPA1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019227.

Structurei

3D structure databases

ProteinModelPortaliQ2KHV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2980. Eukaryota.
COG0705. LUCA.
GeneTreeiENSGT00390000013063.
HOGENOMiHOG000230670.
HOVERGENiHBG082107.
InParanoidiQ2KHV4.
KOiK09650.
OMAiHEMRTNS.
OrthoDBiEOG7NGQBT.
TreeFamiTF313603.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KHV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWRGWAQRG WGCGQAWTLP VCGGSYEELT AALAPSRLLR RRFNFFIQQK
60 70 80 90 100
CGFRKAPRKV EPRRSDTSSE AYKRSALIPP VEETAFYPSP YPIRTLVKPL
110 120 130 140 150
FFTVGFTGCA FGSAAIWQYE SLKSKVQSYF DGIKADWLDS IRPQKEGDFR
160 170 180 190 200
KEINKWWNNL SDGQRTVTGI IAANVFVFCL WRVPSLQRTM IRYFTSNPAS
210 220 230 240 250
KVLCSPMLLS TFSHFSLFHM AANMYVLWSF SSSIVNILGQ EQFMAVYLSA
260 270 280 290 300
GVISTFVSYV CKVATGRYGP SLGASGAIMT VLAAVCTKIP EGRLAIIFLP
310 320 330 340 350
MFTFTAGNAL KAIIAMDTAG MILGWKFFDH AAHLGGALFG IWYITYGHEL
360 370
IWKNREPLVK IWHEMRTNSP KKGGGSK
Length:377
Mass (Da):42,301
Last modified:March 7, 2006 - v1
Checksum:i657E9D56D803203D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41R → G in AAX08693 (PubMed:16305752).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020676 mRNA. Translation: AAX08693.1.
BT026291 mRNA. Translation: ABG81447.1.
BT029889 mRNA. Translation: ABM06139.1.
BC112869 mRNA. Translation: AAI12870.1.
RefSeqiNP_001015596.1. NM_001015596.1.
UniGeneiBt.4331.

Genome annotation databases

EnsembliENSBTAT00000019227; ENSBTAP00000019227; ENSBTAG00000014460.
GeneIDi514191.
KEGGibta:514191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020676 mRNA. Translation: AAX08693.1.
BT026291 mRNA. Translation: ABG81447.1.
BT029889 mRNA. Translation: ABM06139.1.
BC112869 mRNA. Translation: AAI12870.1.
RefSeqiNP_001015596.1. NM_001015596.1.
UniGeneiBt.4331.

3D structure databases

ProteinModelPortaliQ2KHV4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019227.

Proteomic databases

PaxDbiQ2KHV4.
PRIDEiQ2KHV4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000019227; ENSBTAP00000019227; ENSBTAG00000014460.
GeneIDi514191.
KEGGibta:514191.

Organism-specific databases

CTDi55486.

Phylogenomic databases

eggNOGiKOG2980. Eukaryota.
COG0705. LUCA.
GeneTreeiENSGT00390000013063.
HOGENOMiHOG000230670.
HOVERGENiHBG082107.
InParanoidiQ2KHV4.
KOiK09650.
OMAiHEMRTNS.
OrthoDBiEOG7NGQBT.
TreeFamiTF313603.

Miscellaneous databases

NextBioi20871217.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiPARL_BOVIN
AccessioniPrimary (citable) accession number: Q2KHV4
Secondary accession number(s): A1L5A2, Q0V8C8, Q5EA93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 7, 2006
Last modified: May 11, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.