Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent Clp protease proteolytic subunit, mitochondrial

Gene

CLPP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149NucleophileBy similarity1
Active sitei174By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS14.003.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit, mitochondrial (EC:3.4.21.92)
Alternative name(s):
Endopeptidase Clp
Gene namesi
Name:CLPP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000260318? – 272ATP-dependent Clp protease proteolytic subunit, mitochondrial
Transit peptidei1 – 52MitochondrionBy similarityAdd BLAST52

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei196N6-succinyllysineBy similarity1
Modified residuei207N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ2KHU4.
PeptideAtlasiQ2KHU4.
PRIDEiQ2KHU4.

Expressioni

Gene expression databases

BgeeiENSBTAG00000014712.

Interactioni

Subunit structurei

Fourteen CLPP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity. Component of the Clp complex formed by the assembly of two CLPP heptameric rings with two CLPX hexameric rings, giving rise to a symmetrical structure with two central CLPP rings flanked by a CLPX ring at either end of the complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019577.

Structurei

3D structure databases

ProteinModelPortaliQ2KHU4.
SMRiQ2KHU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0840. Eukaryota.
COG0740. LUCA.
GeneTreeiENSGT00390000005830.
HOGENOMiHOG000285833.
HOVERGENiHBG001689.
InParanoidiQ2KHU4.
KOiK01358.
OMAiLFLQSEN.
OrthoDBiEOG091G0KCO.
TreeFamiTF105002.

Family and domain databases

CDDicd07017. S14_ClpP_2. 1 hit.
HAMAPiMF_00444. ClpP. 1 hit.
InterProiView protein in InterPro
IPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom_sf.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiView protein in Pfam
PF00574. CLP_protease. 1 hit.
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiView protein in PROSITE
PS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2KHU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWPKILLRGG RVAAGLCPAL GPRLAARFPP QRTPENRLAP QRNLHATAAR
60 70 80 90 100
ALPLIPIVVE QTGRGERAYD IYSRLLRERI VCVMGPIDDS VASLVIAQLL
110 120 130 140 150
FLQSESNKKP IHMYINSPGG VVTSGLAIYD TMQYILNPIC TWCVGQAASM
160 170 180 190 200
GSLLLAAGTP GMRHSLPNSR IMIHQPSGGA RGQATDIAIQ AEEIMKLKKQ
210 220 230 240 250
LYSIYAKHTK QSLQVIESAM ERDRYMSPME AQEFGILDKV LVHPPQDGED
260 270
EPELVQKEPG EPTAVEPAPA SA
Length:272
Mass (Da):29,704
Last modified:March 7, 2006 - v1
Checksum:i093E2BF04EA98EDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC112880 mRNA. Translation: AAI12881.1.
RefSeqiNP_001039879.1. NM_001046414.2.
UniGeneiBt.54443.

Genome annotation databases

EnsembliENSBTAT00000019577; ENSBTAP00000019577; ENSBTAG00000014712.
GeneIDi535981.
KEGGibta:535981.

Similar proteinsi

Entry informationi

Entry nameiCLPP_BOVIN
AccessioniPrimary (citable) accession number: Q2KHU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: March 7, 2006
Last modified: November 22, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families