Q2KHU0 (SERB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine phosphatase
Short name=PSP
Short name=PSPase
EC=3.1.3.3

Alternative name(s):
L-3-phosphoserine phosphatase
O-phosphoserine phosphohydrolase

Gene names

Name:

PSPH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates By similarity.
Catalytic activity	O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the serB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from sequence or structural similarity. Source: RefGenome
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: RefGenome
Molecular function	calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB phosphoserine phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Phosphoserine phosphatase		PRO_0000244407
Regions
	Region	109 – 110	2	Substrate binding By similarity
Sites
	Active site	20	1	Nucleophile By similarity
	Active site	22	1	Proton donor By similarity
	Metal binding	20	1	Magnesium By similarity
	Metal binding	22	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	179	1	Magnesium By similarity
	Binding site	29	1	Substrate By similarity
	Binding site	65	1	Substrate By similarity
	Binding site	158	1	Substrate By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KHU0 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: B7E176431231DE17	FASTA	225	24,850
	10 20 30 40 50 60 MVSHSELRNL FCSADAVCFD VDSTVIQEEG IDELAKFCGV EDAVSEMTRQ AMGGAVPFKA 70 80 90 100 110 120 ALTQRLALIQ PSREQVQRLL AEHPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA 130 140 150 160 170 180 SKLNIPSTNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIVMVGDG 190 200 210 220 ATDMEACPPA DAFIGFGGNV IRQQVKDNAE WYITDFVELL GALEE

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thymus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC112884 mRNA. Translation: AAI12885.1.
IPI	IPI00701167.
RefSeq	NP_001039820.1. NM_001046355.1.
UniGene	Bt.25099.
3D structure databases
ProteinModelPortal	Q2KHU0.
SMR	Q2KHU0. Positions 4-225.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KHU0.
Proteomic databases
PRIDE	Q2KHU0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000017392; ENSBTAP00000017392; ENSBTAG00000013081.
GeneID	533630.
KEGG	bta:533630.
Organism-specific databases
CTD	5723.
Phylogenomic databases
eggNOG	maNOG12671.
GeneTree	ENSGT00390000003115.
HOVERGEN	HBG057486.
InParanoid	Q2KHU0.
OrthoDB	EOG4NVZM5.
PhylomeDB	Q2KHU0.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR006383. HAD-SF_hydro_IB_PSP-like. IPR023190. Pser_Pase_dom_2. IPR004469. SerB. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits. G3DSA:1.10.150.210. Pser_Pase_dom_2. 1 hit.
KO	K01079.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01488. HAD-SF-IB. 1 hit. TIGR00338. SerB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SERB_BOVIN

Accession

Primary (citable) accession number: Q2KHU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 7, 2006
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program