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Q2KE51

- MASZ_RHIEC

UniProt

Q2KE51 - MASZ_RHIEC

Protein

Malate synthase G

Gene

glcB

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei274 – 2741Acetyl-CoAUniRule annotation
    Binding sitei311 – 3111Acetyl-CoAUniRule annotation
    Active sitei338 – 3381Proton acceptorUniRule annotation
    Binding sitei338 – 3381GlyoxylateUniRule annotation
    Metal bindingi430 – 4301MagnesiumUniRule annotation
    Binding sitei430 – 4301GlyoxylateUniRule annotation
    Metal bindingi458 – 4581MagnesiumUniRule annotation
    Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei629 – 6291Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciRETL347834:GJJ0-52-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:RHE_CH00052
    OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
    Taxonomic identifieri347834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
    ProteomesiUP000001936: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 723723Malate synthase GPRO_1000056921Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei615 – 6151Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi347834.RHE_CH00052.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2KE51.
    SMRiQ2KE51. Positions 9-722.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni123 – 1242Acetyl-CoA bindingUniRule annotation
    Regioni455 – 4584Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiARTENVD.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2KE51-1 [UniParc]FASTAAdd to Basket

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    MSRIDKNGLA IETILHDFLT QEVLPGLAID ADRFFADFSA IVHDLAPRNR    50
    ALLVKRDELQ TKIDDWYRQH GAPSDMDDYQ SFLRSIGYLL PEGSDFQVST 100
    ANVDPEIASI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIPEND 150
    GAEKGKGYNP KRGEKVIAWV RDFLDAAAPL QDSRWKDVGS FFVKDGMLVV 200
    RSVDGEQTML GDGGHFAGYR GDAAAPAHIL LKNNGIHIDI VIDPTTAIGK 250
    ADPAHISDVW LESAITTIMD CEDSIAAVDA EDKVVVYRNW LGLMKGDLQE 300
    EVAKGGTTFL RKLNPDLDYT SPDGASFELH RRSLMLVRNV GHLMTNPAIL 350
    DKDGNEVPEG IMDAVITGLI ALYDIGPTGR KKNSRTGSMY VVKPKMHGPE 400
    EVAFAVEIFS RVEDALGMAR NTIKMGIMDE ERRTTINLKE CIRAARERVV 450
    FINTGFLDRT GDEIHTSMEA GPMIRKGDMR QAAWISAYEN WNVDIGLECG 500
    LSGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH 550
    YHRVNVAKVQ QGLKDRARAR LSDILSVPVA VRPNWTPEEI QRELDNNAQG 600
    ILGYVVRWID QGVGCSKVPD INNIGLMEDR ATLRISAQHM ANWLHHKVVT 650
    EAQIVETMKR MAAIVDRQNE SDPAYRPMAG NFDDSIAFQA ALDLVLKGRE 700
    QPNGYTEPVL HRRRLELKAK QSA 723
    Length:723
    Mass (Da):79,899
    Last modified:March 7, 2006 - v1
    Checksum:i01C9C009C1A9E813
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000133 Genomic DNA. Translation: ABC88885.1.
    RefSeqiWP_011423456.1. NC_007761.1.
    YP_467612.1. NC_007761.1.

    Genome annotation databases

    EnsemblBacteriaiABC88885; ABC88885; RHE_CH00052.
    GeneIDi3893196.
    KEGGiret:RHE_CH00052.
    PATRICi23081380. VBIRhiEtl108884_0382.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000133 Genomic DNA. Translation: ABC88885.1 .
    RefSeqi WP_011423456.1. NC_007761.1.
    YP_467612.1. NC_007761.1.

    3D structure databases

    ProteinModelPortali Q2KE51.
    SMRi Q2KE51. Positions 9-722.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 347834.RHE_CH00052.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC88885 ; ABC88885 ; RHE_CH00052 .
    GeneIDi 3893196.
    KEGGi ret:RHE_CH00052.
    PATRICi 23081380. VBIRhiEtl108884_0382.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi ARTENVD.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci RETL347834:GJJ0-52-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CFN 42 / ATCC 51251.

    Entry informationi

    Entry nameiMASZ_RHIEC
    AccessioniPrimary (citable) accession number: Q2KE51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3