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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi487MagnesiumUniRule annotation1
Metal bindingi493MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:RHE_CH00111
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000001936 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003297991 – 712Polyribonucleotide nucleotidyltransferaseAdd BLAST712

Proteomic databases

PRIDEiQ2KE00.

Interactioni

Protein-protein interaction databases

STRINGi347834.RHE_CH00111.

Structurei

3D structure databases

ProteinModelPortaliQ2KE00.
SMRiQ2KE00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini554 – 613KHUniRule annotationAdd BLAST60
Domaini623 – 691S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRYMHNYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2KE00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDTHTVEIE WAGRPLKLET GKIARQADGA VLATYGETVV LATVVSAKAP
60 70 80 90 100
KPGQDFFPLT VNYQEKTYAA GKIPGGYFKR EGRPSENETL VSRLIDRPIR
110 120 130 140 150
PLFPEGYKND TQVVVTVVQH DLENNPDILS MIASSAALTL SGVPFMGPVG
160 170 180 190 200
GARVGYINGE YVLNPHLDEM DESSLDLVVA GTYDAVLMVE SEAKELPEDV
210 220 230 240 250
MLGAVMFGHK GFQPVLDAII KLAEVAAKEP RDFQPEDYSE LEGEMLKHFE
260 270 280 290 300
AELREAYKIT QKADRYAAVD AVKAKVKAHF LPEEGEAKYT AEEVGAIFKH
310 320 330 340 350
LQAKIVRWNI LDTKSRIDGR DLETVRPIVS EVGLLPRTHG SALFTRGETQ
360 370 380 390 400
AIVVATLGTG EDEQYVDSLT GMYKERFLLH YNFPPFSVGE TGRMGSPGRR
410 420 430 440 450
EIGHGKLAWR AIRPMLPSAE QFPYTLRVVS EITESNGSSS MATVCGTSLA
460 470 480 490 500
LMDAGVPLAK PVAGIAMGLI LEGDRFAVLS DILGDEDHLG DMDFKVAGTA
510 520 530 540 550
DGITSLQMDI KIAGITEEIM KVALGQAKGG RAHILGEMAK AITESRGQLG
560 570 580 590 600
EFAPRIEVMN IPVDKIREVI GSGGKVIREI VEKTGAKINI EDDGTVKIAS
610 620 630 640 650
SSGKEIEAAR KWIHSIVAEP EIGQIYEGTV VKTADFGAFV NFFGARDGLV
660 670 680 690 700
HISQLASERV AKTTDVVKEG DKVWVKLLGF DERGKVRLSM KVVDQATGQE
710
IPNEKKKEEA AE
Length:712
Mass (Da):77,483
Last modified:March 7, 2006 - v1
Checksum:iB518696E2D51255F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC88936.1.
RefSeqiWP_011423507.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC88936; ABC88936; RHE_CH00111.
GeneIDi24298735.
KEGGiret:RHE_CH00111.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNP_RHIEC
AccessioniPrimary (citable) accession number: Q2KE00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 7, 2006
Last modified: June 7, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families