Q2KDD4 (DUT_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	RHE_CH00330

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_1000015500
Regions
	Region	76 – 78	3	Substrate binding By similarity
	Region	93 – 95	3	Substrate binding By similarity
Sites
	Binding site	89	1	Substrate By similarity
	Binding site	103	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KDD4 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 3B16A4C63D5AC3EF	FASTA	156	16,285
	10 20 30 40 50 60 MTIHHDTPPT LNLIRLANGE GLDLPAYESK GAAGMDLRAA VDGDAPLTLA PGERALLPTG 70 80 90 100 110 120 FIFEIPEGFE GQVRPRSGLA FKHGITCLNS PGTVDSDYRG EVKVLLANLG EEAFVIERGM 130 140 150 RIAQMVIAPV TQARVTEIAA ASETARGAGG FGSTGV

References

[1]

"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons." Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G. Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed: 16505379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000133 Genomic DNA. Translation: ABC89152.1.
RefSeq	YP_467879.1. NC_007761.1.
3D structure databases
ProteinModelPortal	Q2KDD4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KDD4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3891120.
GenomeReviews	Gene locus RHE_CH00330 in contig CP000133_GR.
KEGG	ret:RHE_CH00330.
PATRIC	23081958. VBIRhiEtl108884_0663.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	LDLRACI.
PhylomeDB	Q2KDD4.
ProtClustDB	PRK00601.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_RHIEC

Accession

Primary (citable) accession number: Q2KDD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program