Reviewed, UniProtKB/Swiss-Prot Q2KCH8 (CHEB1_RHIEC)

Names and origin

Protein names

Recommended name:
    Probable chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	RHE_CH00642
ORF Names:	RHE_CH00643

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Probable chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000239043
Regions
	Domain	6 – 123	118	Response regulatory
	Domain	150 – 342	193	CheB-type methylesterase
Sites
	Active site	162	1	By similarity
	Active site	188	1	By similarity
	Active site	284	1	By similarity
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KCH8-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 7CA9F79F8D7EF8E3	FASTA	347	36,566
	10 20 30 40 50 60 MSAPARVLVV DDSPTMRGLI TAVLSSDPEV SVIGQAGDAL EAREAIKRLN PDVVTLDIEM 70 80 90 100 110 120 PNMNGLDFLE KIMTLRPMPV IMVSTMTHRG AEATLAALEI GAFDCVGKPG PGEHRPFGDL 130 140 150 160 170 180 AEKVKAAART QRQFSQPAAA VAPPPSVADF RVGRKIVAIG SSTGGVEALI AVLQKFPANC 190 200 210 220 230 240 PPTVITQHMP PTFTKSFAER LNRLCAPVVQ EATDGARLEI GKIYLAPGGE RHLQVSGGSA 250 260 270 280 290 300 PCCRLVDRAP VNGHRPSVDV LFDSVAELAG RNAVGVILTG MGRDGAAGLL KMRHAGARTL 310 320 330 340 GQTEKTCVVY GMPRVAHELG AVEQQLPLTA IGEEILKLTA ARKEGTE

References

[1]

"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons." Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G. Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed: 16505379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000133 Genomic DNA. Translation: ABC89458.1.
RefSeq	YP_468185.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KCH8.
Genome annotation databases
GeneID	3893041.
GenomeReviews	Gene locus RHE_CH00642 in contig CP000133_GR.
KEGG	ret:RHE_CH00642.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KCH8.
OMA	GKIYLAP.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_RHIEC

Accession

Primary (citable) accession number: Q2KCH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)