Q2KBR2 (DXS_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	RHE_CH00913

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP]

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 638	638	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256467

Sequences

	Sequence		Length	Mass (Da)
	Q2KBR2 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: C5BE295EAE9F0D1C	FASTA	638	68,387
	10 20 30 40 50 60 MTHPPKTPLL DQVTYPADLR KLEDRDLPQL AREVRDEMID AVSRTGGHLG AGLGVVELTI 70 80 90 100 110 120 AIHSVFDTPN DRLIFDVGHQ CYPHKILTGR RDRIRTLRQE NGLSGFTRRA ESEYDPFGAA 130 140 150 160 170 180 HSSTSISAGL GMAIAADLEK TDRRVIAVIG DGAMSAGMAY EALNNAGALD ARLIVILNDN 190 200 210 220 230 240 DMSIAPPTGA MSAYLARLAS GRTYMGFRDF GKKLTAYLGK NIDRAITRAV EHARGYVTGG 250 260 270 280 290 300 TMFEEMGFYH IGPIDGHSFD HLLPVLRNVR DNARGPVLIH VVTQKGKGYP PAEAAADKYH 310 320 330 340 350 360 GVNKFDVITG AQARVKPNAP SYTSVFAEAL VQEAALDDKI VGITAAMPNG TGLDKLAEAF 370 380 390 400 410 420 PSRCFDVGIA EQHAVTFAAG LAAEGYKPFA ALYSTFLQRA YDQVVHDVAI QGLPVRFPID 430 440 450 460 470 480 RAGFVGADGP THAGSFDTAF LATLPGFVVM AAADEAELKH MVRTAAAYDA GPISFRYPRG 490 500 510 520 530 540 EGVGVDMPAR GEILQIGKGR IVKEGTKVAL LSFGTRLADC LLASEDLEAA GLSTTVADAR 550 560 570 580 590 600 FAKPLDHELL RQLARHHEML ITVEEGSVGG FGSQVMQYLS SEGLLDNGLK IRSLVMPDIW 610 620 630 MEQAKPEAMN AHAGLDRAGI VSTVFRALRR GVAVGVAG

References

[1]

"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons." Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G. Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed: 16505379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000133 Genomic DNA. Translation: ABC89724.1.
RefSeq	YP_468451.1. NC_007761.1.
3D structure databases
ProteinModelPortal	Q2KBR2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2KBR2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3891660.
GenomeReviews	Gene locus RHE_CH00913 in contig CP000133_GR.
KEGG	ret:RHE_CH00913.
PATRIC	23083142. VBIRhiEtl108884_1247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	EAMNAHA.
PhylomeDB	Q2KBR2.
ProtClustDB	PRK05444.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_RHIEC

Accession

Primary (citable) accession number: Q2KBR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program