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Q2KA25

- GLYA_RHIEC

UniProt

Q2KA25 - GLYA_RHIEC

Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411Pyridoxal phosphateUniRule annotation
    Binding sitei61 – 611Pyridoxal phosphateUniRule annotation
    Binding sitei63 – 631SubstrateUniRule annotation
    Binding sitei70 – 701SubstrateUniRule annotation
    Binding sitei71 – 711Pyridoxal phosphateUniRule annotation
    Binding sitei105 – 1051Pyridoxal phosphateUniRule annotation
    Binding sitei127 – 1271Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei182 – 1821Pyridoxal phosphateUniRule annotation
    Binding sitei210 – 2101Pyridoxal phosphateUniRule annotation
    Binding sitei235 – 2351Pyridoxal phosphateUniRule annotation
    Binding sitei242 – 2421Pyridoxal phosphateUniRule annotation
    Binding sitei268 – 2681Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei368 – 3681Pyridoxal phosphateUniRule annotation

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciRETL347834:GJJ0-1517-MONOMER.
    UniPathwayiUPA00193.
    UPA00288; UER01023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
    Short name:
    SHMTUniRule annotation
    Short name:
    Serine methylaseUniRule annotation
    Gene namesi
    Name:glyAUniRule annotation
    Ordered Locus Names:RHE_CH01508
    OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
    Taxonomic identifieri347834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
    ProteomesiUP000001936: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 432432Serine hydroxymethyltransferasePRO_1000006303Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei236 – 2361N6-(pyridoxal phosphate)lysineUniRule annotation

    Proteomic databases

    PRIDEiQ2KA25.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi347834.RHE_CH01508.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2KA25.
    SMRiQ2KA25. Positions 12-423.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni131 – 1333Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the SHMT family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0112.
    HOGENOMiHOG000239404.
    KOiK00600.
    OMAiCAYVNVQ.
    OrthoDBiEOG6Z0QB2.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2KA25-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNASTESFF NRSLADVDPE IFGAIGKELG RQRHEIELIA SENIVSRAVL    50
    EAQGSIMTNK YAEGYPGKRY YGGCQFVDIA EELAIERAKK LFGVNFANVQ 100
    PNSGSQMNQA VFLALLQPGD TFMGLDLNSG GHLTHGSPVN MSGKWFNVVS 150
    YGVREGDNLL DMDEVARKAE EHKPKVIIAG GTAYSRIWDW KRFREIADSV 200
    GAYLMVDMAH IAGLVAGGQH PSPFPHCHVA TTTTHKSLRG PRGGVILTNE 250
    EDLAKKFNSA VFPGLQGGPL MHIIAAKAVA FGEALQPEFK EYAAQIVKNA 300
    RALAETLIAG GLDVVSGGTD NHLMLVDLRK KNATGKRAEA ALGRAYITCN 350
    KNGIPFDPEK PFVTSGVRLG APAGTTRGFK EAEFREIGNL IVEVLDGLKV 400
    ANSDDGNAAV EAAVRGKVVN LTDRFPMYDY MG 432
    Length:432
    Mass (Da):46,598
    Last modified:March 7, 2006 - v1
    Checksum:iF8CF2FDAD295117D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000133 Genomic DNA. Translation: ABC90311.1.
    RefSeqiWP_011424840.1. NC_007761.1.
    YP_469038.1. NC_007761.1.

    Genome annotation databases

    EnsemblBacteriaiABC90311; ABC90311; RHE_CH01508.
    GeneIDi3890943.
    KEGGiret:RHE_CH01508.
    PATRICi23084429. VBIRhiEtl108884_1886.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000133 Genomic DNA. Translation: ABC90311.1 .
    RefSeqi WP_011424840.1. NC_007761.1.
    YP_469038.1. NC_007761.1.

    3D structure databases

    ProteinModelPortali Q2KA25.
    SMRi Q2KA25. Positions 12-423.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 347834.RHE_CH01508.

    Proteomic databases

    PRIDEi Q2KA25.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC90311 ; ABC90311 ; RHE_CH01508 .
    GeneIDi 3890943.
    KEGGi ret:RHE_CH01508.
    PATRICi 23084429. VBIRhiEtl108884_1886.

    Phylogenomic databases

    eggNOGi COG0112.
    HOGENOMi HOG000239404.
    KOi K00600.
    OMAi CAYVNVQ.
    OrthoDBi EOG6Z0QB2.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    UPA00288 ; UER01023 .
    BioCyci RETL347834:GJJ0-1517-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CFN 42 / ATCC 51251.

    Entry informationi

    Entry nameiGLYA_RHIEC
    AccessioniPrimary (citable) accession number: Q2KA25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3