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Q2KA25

- GLYA_RHIEC

UniProt

Q2KA25 - GLYA_RHIEC

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Protein

Serine hydroxymethyltransferase

Gene
glyA, RHE_CH01508
Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

Pyridoxal phosphate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411Pyridoxal phosphate By similarity
Binding sitei61 – 611Pyridoxal phosphate By similarity
Binding sitei63 – 631Substrate By similarity
Binding sitei70 – 701Substrate By similarity
Binding sitei71 – 711Pyridoxal phosphate By similarity
Binding sitei105 – 1051Pyridoxal phosphate By similarity
Binding sitei127 – 1271Substrate; via carbonyl oxygen By similarity
Binding sitei182 – 1821Pyridoxal phosphate By similarity
Binding sitei210 – 2101Pyridoxal phosphate By similarity
Binding sitei235 – 2351Pyridoxal phosphate By similarity
Binding sitei242 – 2421Pyridoxal phosphate By similarity
Binding sitei268 – 2681Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei368 – 3681Pyridoxal phosphate By similarity

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-1517-MONOMER.
UniPathwayiUPA00193.
UPA00288; UER01023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferase (EC:2.1.2.1)
Short name:
SHMT
Short name:
Serine methylase
Gene namesi
Name:glyA
Ordered Locus Names:RHE_CH01508
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000001936: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Serine hydroxymethyltransferaseUniRule annotationPRO_1000006303Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361N6-(pyridoxal phosphate)lysine By similarity

Proteomic databases

PRIDEiQ2KA25.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi347834.RHE_CH01508.

Structurei

3D structure databases

ProteinModelPortaliQ2KA25.
SMRiQ2KA25. Positions 12-423.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 1333Substrate binding By similarity

Sequence similaritiesi

Belongs to the SHMT family.

Phylogenomic databases

eggNOGiCOG0112.
HOGENOMiHOG000239404.
KOiK00600.
OMAiCAYVNVQ.
OrthoDBiEOG6Z0QB2.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2KA25-1 [UniParc]FASTAAdd to Basket

« Hide

MTNASTESFF NRSLADVDPE IFGAIGKELG RQRHEIELIA SENIVSRAVL    50
EAQGSIMTNK YAEGYPGKRY YGGCQFVDIA EELAIERAKK LFGVNFANVQ 100
PNSGSQMNQA VFLALLQPGD TFMGLDLNSG GHLTHGSPVN MSGKWFNVVS 150
YGVREGDNLL DMDEVARKAE EHKPKVIIAG GTAYSRIWDW KRFREIADSV 200
GAYLMVDMAH IAGLVAGGQH PSPFPHCHVA TTTTHKSLRG PRGGVILTNE 250
EDLAKKFNSA VFPGLQGGPL MHIIAAKAVA FGEALQPEFK EYAAQIVKNA 300
RALAETLIAG GLDVVSGGTD NHLMLVDLRK KNATGKRAEA ALGRAYITCN 350
KNGIPFDPEK PFVTSGVRLG APAGTTRGFK EAEFREIGNL IVEVLDGLKV 400
ANSDDGNAAV EAAVRGKVVN LTDRFPMYDY MG 432
Length:432
Mass (Da):46,598
Last modified:March 7, 2006 - v1
Checksum:iF8CF2FDAD295117D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000133 Genomic DNA. Translation: ABC90311.1.
RefSeqiWP_011424840.1. NC_007761.1.
YP_469038.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC90311; ABC90311; RHE_CH01508.
GeneIDi3890943.
KEGGiret:RHE_CH01508.
PATRICi23084429. VBIRhiEtl108884_1886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000133 Genomic DNA. Translation: ABC90311.1 .
RefSeqi WP_011424840.1. NC_007761.1.
YP_469038.1. NC_007761.1.

3D structure databases

ProteinModelPortali Q2KA25.
SMRi Q2KA25. Positions 12-423.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 347834.RHE_CH01508.

Proteomic databases

PRIDEi Q2KA25.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC90311 ; ABC90311 ; RHE_CH01508 .
GeneIDi 3890943.
KEGGi ret:RHE_CH01508.
PATRICi 23084429. VBIRhiEtl108884_1886.

Phylogenomic databases

eggNOGi COG0112.
HOGENOMi HOG000239404.
KOi K00600.
OMAi CAYVNVQ.
OrthoDBi EOG6Z0QB2.

Enzyme and pathway databases

UniPathwayi UPA00193 .
UPA00288 ; UER01023 .
BioCyci RETL347834:GJJ0-1517-MONOMER.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00051. SHMT.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view ]
PANTHERi PTHR11680. PTHR11680. 1 hit.
Pfami PF00464. SHMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000412. SHMT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00096. SHMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.

Entry informationi

Entry nameiGLYA_RHIEC
AccessioniPrimary (citable) accession number: Q2KA25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: September 3, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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