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Protein

Dihydroxy-acid dehydratase

Gene

ilvD

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvI)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE2)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvI)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE2)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi195 – 1951Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-1715-MONOMER.
UniPathwayiUPA00047; UER00057.
UPA00049; UER00061.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxy-acid dehydrataseUniRule annotation (EC:4.2.1.9UniRule annotation)
Short name:
DADUniRule annotation
Gene namesi
Name:ilvDUniRule annotation
Ordered Locus Names:RHE_CH01704
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000001936 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Dihydroxy-acid dehydratasePRO_1000001043Add
BLAST

Proteomic databases

PRIDEiQ2K9I7.

Interactioni

Protein-protein interaction databases

STRINGi347834.RHE_CH01704.

Family & Domainsi

Sequence similaritiesi

Belongs to the IlvD/Edd family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C01. Bacteria.
COG0129. LUCA.
HOGENOMiHOG000173155.
KOiK01687.
OMAiQGRNMAG.
OrthoDBiEOG6MSS24.

Family and domain databases

HAMAPiMF_00012. IlvD.
InterProiIPR015928. Aconitase/3IPM_dehydase_swvl.
IPR004404. DihydroxyA_deHydtase.
IPR000581. DiOHA_6PGluconate_deHydtase.
IPR020558. DiOHA_6PGluconate_deHydtase_CS.
[Graphical view]
PANTHERiPTHR21000. PTHR21000. 2 hits.
PfamiPF00920. ILVD_EDD. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00110. ilvD. 1 hit.
PROSITEiPS00886. ILVD_EDD_1. 1 hit.
PS00887. ILVD_EDD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2K9I7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAYRSRTTT HGRNMAGARG LWRATGMKDS DFGKPIIAVV NSFTQFVPGH
60 70 80 90 100
VHLKDLGQLV AREIEAAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRELI
110 120 130 140 150
ADSVEYMVNA HCADAMVCIS NCDKITPGML MASLRLNIPT VFVSGGPMEA
160 170 180 190 200
GKVVLHGKQH ALDLVDAMVA AADDKISDED VKVIERSACP TCGSCSGMFT
210 220 230 240 250
ANSMNCLTEA LGLSLPGNGS TLATHADRKR LFVEAGHLIV DLARRYYEQD
260 270 280 290 300
DVKALPRTIA SKQAFENAMA LDIAMGGSTN TVLHILAAAH EGEVDFTMAD
310 320 330 340 350
IDALSRRVPC LSKVAPAKSD VHMEDVHRAG GIMSILGELD KGGLLNRDCP
360 370 380 390 400
TVHAETLGDA IDRWDITRTT SDTVRDFYRA APGGIPTQVA FSQEARWDEL
410 420 430 440 450
DTDRQNGVIR SVEHPFSKDG GLAVLKGNLA VDGCIVKTAG VDESILKFSG
460 470 480 490 500
PARVFESQDA SVKAILANEI KAGDVVVIRY EGPKGGPGMQ EMLYPTSYLK
510 520 530 540 550
SKGLGKACAL ITDGRFSGGT SGLSIGHVSP EAANGGTIGL VREGDIIDID
560 570 580 590 600
IPNRTISLRV GEAELAARRA EQDARGWRPT EVRKRNVTTA LKAYAAFATS
610
ADRGAVRDLD AR
Length:612
Mass (Da):65,216
Last modified:March 7, 2006 - v1
Checksum:iC28BA867988E8E38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC90499.1.
RefSeqiWP_011424997.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC90499; ABC90499; RHE_CH01704.
KEGGiret:RHE_CH01704.
PATRICi23084849. VBIRhiEtl108884_2088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC90499.1.
RefSeqiWP_011424997.1. NC_007761.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347834.RHE_CH01704.

Proteomic databases

PRIDEiQ2K9I7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC90499; ABC90499; RHE_CH01704.
KEGGiret:RHE_CH01704.
PATRICi23084849. VBIRhiEtl108884_2088.

Phylogenomic databases

eggNOGiENOG4105C01. Bacteria.
COG0129. LUCA.
HOGENOMiHOG000173155.
KOiK01687.
OMAiQGRNMAG.
OrthoDBiEOG6MSS24.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00057.
UPA00049; UER00061.
BioCyciRETL347834:GJJ0-1715-MONOMER.

Family and domain databases

HAMAPiMF_00012. IlvD.
InterProiIPR015928. Aconitase/3IPM_dehydase_swvl.
IPR004404. DihydroxyA_deHydtase.
IPR000581. DiOHA_6PGluconate_deHydtase.
IPR020558. DiOHA_6PGluconate_deHydtase_CS.
[Graphical view]
PANTHERiPTHR21000. PTHR21000. 2 hits.
PfamiPF00920. ILVD_EDD. 1 hit.
[Graphical view]
SUPFAMiSSF52016. SSF52016. 1 hit.
TIGRFAMsiTIGR00110. ilvD. 1 hit.
PROSITEiPS00886. ILVD_EDD_1. 1 hit.
PS00887. ILVD_EDD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.

Entry informationi

Entry nameiILVD_RHIEC
AccessioniPrimary (citable) accession number: Q2K9I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: February 17, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.