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Q2K8V9

- LIPA_RHIEC

UniProt

Q2K8V9 - LIPA_RHIEC

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Protein

Lipoyl synthase

Gene

lipA

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi91 – 911Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-1952-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:RHE_CH01941
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000001936: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Lipoyl synthasePRO_1000012259Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi347834.RHE_CH01941.

Structurei

3D structure databases

ProteinModelPortaliQ2K8V9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPEEPYNT.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2K8V9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTILDTINP DAKRVRHPEK AHRPDTEVMR KPDWIRVKAP TSKGYAETRA
60 70 80 90 100
IVKEHKLVTV CEEAGCPNIG ECWDKKHATF MIMGEICTRA CAFCNVATGK
110 120 130 140 150
PNALDMDEPE NVAKAVREMG LSHVVITSVD RDDLEDGGAE HFEKVIWAIR
160 170 180 190 200
SASPATTIEI LTPDFLKKPG ALERVVAAKP DVFNHNMETV AGNYLTVRPG
210 220 230 240 250
ARYFHSIRLL QRVKELDPTM FTKSGIMVGL GEERNEVLQL MDDLRTADVD
260 270 280 290 300
FLTIGQYLQP TRKHHKVESF VTPEEFKSYE TVAYSKGFLM VASSPLTRSS
310 320
HHAGDDFARL RAAREKKLLM AAE
Length:323
Mass (Da):36,218
Last modified:March 7, 2006 - v1
Checksum:i22B1C443F471E041
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921A → R in CAA72400. (PubMed:9141657)Curated
Sequence conflicti100 – 1001K → Q in CAA72400. (PubMed:9141657)Curated
Sequence conflicti118 – 1225EMGLS → RWAQ in CAA72400. (PubMed:9141657)Curated
Sequence conflicti140 – 1401E → Q in CAA72400. (PubMed:9141657)Curated
Sequence conflicti317 – 3182KL → NV in CAA72400. (PubMed:9141657)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11708 Genomic DNA. Translation: CAA72400.1.
CP000133 Genomic DNA. Translation: ABC90727.1.
RefSeqiYP_469454.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC90727; ABC90727; RHE_CH01941.
GeneIDi3893979.
KEGGiret:RHE_CH01941.
PATRICi23085366. VBIRhiEtl108884_2339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11708 Genomic DNA. Translation: CAA72400.1 .
CP000133 Genomic DNA. Translation: ABC90727.1 .
RefSeqi YP_469454.1. NC_007761.1.

3D structure databases

ProteinModelPortali Q2K8V9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 347834.RHE_CH01941.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC90727 ; ABC90727 ; RHE_CH01941 .
GeneIDi 3893979.
KEGGi ret:RHE_CH01941.
PATRICi 23085366. VBIRhiEtl108884_2339.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi PEEPYNT.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci RETL347834:GJJ0-1952-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and transcriptional analysis of the lipA (lipoic acid synthetase) gene from Rhizobium etli."
    Tate R., Riccio A., Iaccarino M., Patriarca E.J.
    FEMS Microbiol. Lett. 149:165-172(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CE3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.

Entry informationi

Entry nameiLIPA_RHIEC
AccessioniPrimary (citable) accession number: Q2K8V9
Secondary accession number(s): O05941
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3