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Q2K8D5 (Q2K8D5_RHIEC) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase RuleBase RU003777 HAMAP-Rule MF_00168
EC=2.4.2.29 RuleBase RU003777 HAMAP-Rule MF_00168
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168 EMBL ABC90901.1
Ordered Locus Names:RHE_CH02117 EMBL ABC90901.1
OrganismRhizobium etli (strain CFN 42 / ATCC 51251) [Complete proteome] [HAMAP] EMBL ABC90901.1
Taxonomic identifier347834 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium ›

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. RuleBase RU003777 HAMAP-Rule MF_00168 SAAS SAAS004803

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. SAAS SAAS004803 HAMAP-Rule MF_00168

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. SAAS SAAS004803 HAMAP-Rule MF_00168

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. RuleBase RU003777 SAAS SAAS004803 HAMAP-Rule MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. SAAS SAAS004803 HAMAP-Rule MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. RuleBase RU003777 HAMAP-Rule MF_00168

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site931Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3071Zinc By similarity HAMAP-Rule MF_00168
Metal binding3361Zinc By similarity HAMAP-Rule MF_00168
Binding site941Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
Q2K8D5 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 155FE60FCCC6C4E0

FASTA37641,844
        10         20         30         40         50         60 
MTENFQFTLK KTDAGARLGE ISMPRGEIRT PAFMPVGTVG TVKAMYLDQV RETGADIILG 

        70         80         90        100        110        120 
NTYHLMLRPT AERVARLGGL HKLIRWEHPI LTDSGGFQVM SLSGLRKLDE QGVTFKSHVD 

       130        140        150        160        170        180 
GSLHHMSPER SIEIQGLLGS DIQMQLDECV ALPAEPKEIE RAMEMSLRWA ERCRVAFGEQ 

       190        200        210        220        230        240 
PGKAMFGIVQ GGDIPALRIR SAEALSQLDL KGYAVGGLAV GEPQDVMLRM LETTLPVLPS 

       250        260        270        280        290        300 
EKPRYLMGVG TPDDILKSVA RGIDMFDCVM PTRSGRHGLA FTRRGKVNIR NARHAEDMRP 

       310        320        330        340        350        360 
LDEQSNCPAS RDYSRAYLHH LVRANEALGG MLLSWHNLAY YQELMQGIRK AIAEGRFADF 

       370 
MAETMEEWAR GDLDPV

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References

[1]"The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons."
Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I., Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A., Jimenez-Jacinto V., Collado-Vides J., Davila G.
Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFN 42 / ATCC 51251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000133 Genomic DNA. Translation: ABC90901.1.
RefSeqYP_469628.1. NC_007761.1.

3D structure databases

ProteinModelPortalQ2K8D5.
SMRQ2K8D5. Positions 5-360.
ModBaseSearch...

Protein-protein interaction databases

STRING347834.RHE_CH02117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC90901; ABC90901; RHE_CH02117.
GeneID3892195.
KEGGret:RHE_CH02117.
PATRIC23085736. VBIRhiEtl108884_2523.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycRETL347834:GJJ0-2128-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ2K8D5_RHIEC
AccessionPrimary (citable) accession number: Q2K8D5
Entry history
Integrated into UniProtKB/TrEMBL: March 7, 2006
Last sequence update: March 7, 2006
Last modified: May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info