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Protein

L-threonine 3-dehydrogenase

Gene

tdh

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD+-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.UniRule annotation

Catalytic activityi

L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: L-threonine degradation via oxydo-reductase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycine from L-threonine.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-threonine 3-dehydrogenase (tdh)
  2. 2-amino-3-ketobutyrate coenzyme A ligase (kbl)
This subpathway is part of the pathway L-threonine degradation via oxydo-reductase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-threonine, the pathway L-threonine degradation via oxydo-reductase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Zinc 1; catalyticUniRule annotation
Active sitei44 – 441Charge relay systemUniRule annotation
Active sitei47 – 471Charge relay systemUniRule annotation
Metal bindingi67 – 671Zinc 1; via tele nitrogen; catalyticUniRule annotation
Metal bindingi68 – 681Zinc 1; catalyticUniRule annotation
Metal bindingi97 – 971Zinc 2UniRule annotation
Metal bindingi100 – 1001Zinc 2UniRule annotation
Metal bindingi103 – 1031Zinc 2UniRule annotation
Metal bindingi111 – 1111Zinc 2UniRule annotation
Sitei152 – 1521Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atomUniRule annotation
Binding sitei179 – 1791NAD; via amide nitrogenUniRule annotation
Binding sitei199 – 1991NADUniRule annotation
Binding sitei204 – 2041NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi266 – 2683NADUniRule annotation
Nucleotide bindingi290 – 2912NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-2964-MONOMER.
UniPathwayiUPA00046; UER00505.

Names & Taxonomyi

Protein namesi
Recommended name:
L-threonine 3-dehydrogenaseUniRule annotation (EC:1.1.1.103UniRule annotation)
Short name:
TDHUniRule annotation
Gene namesi
Name:tdhUniRule annotation
Ordered Locus Names:RHE_CH02951
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000001936 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345L-threonine 3-dehydrogenasePRO_1000051648Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi347834.RHE_CH02951.

Structurei

3D structure databases

ProteinModelPortaliQ2K618.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the zinc-containing alcohol dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPQ. Bacteria.
COG1063. LUCA.
HOGENOMiHOG000294686.
KOiK00060.
OMAiINPHRLE.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
HAMAPiMF_00627. Thr_dehydrog. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR004627. L-Threonine_3-DHase.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00692. tdh. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2K618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNMMKALVK SKPEVGLWME NVPVPEVGPN DVLIRVKKSA ICGTDVHIWN
60 70 80 90 100
WDQWAQKTIP VPMVVGHEFS GEIAEIGSAV TRYHVGERVS GEGHIVCGKC
110 120 130 140 150
RNCRAGRGHL CRNTLGVGVN RPGSFGEFVC IPESNVVPIP DDISDEIAAI
160 170 180 190 200
FDPFGNAVHT ALSFDLVGED VLVTGAGPIG IMGALVAKRS GARKVVITDI
210 220 230 240 250
NPHRLDLARK LGIDHVVDAS KENLADVMKA IGMTEGFDVG LEMSGAAPAF
260 270 280 290 300
RDMIDKMNNG GKIAILGIAP AGFEIDWNKV IFKMLNLKGI YGREMFETWY
310 320 330 340
KMIAFVQGGL DLSPIITHRI KIDDFRDGFE AMRSGNSGKV VMDWM
Length:345
Mass (Da):37,530
Last modified:March 7, 2006 - v1
Checksum:i0E0B1A9CC9C1884C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC91718.1.
RefSeqiWP_011426194.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC91718; ABC91718; RHE_CH02951.
GeneIDi24299445.
KEGGiret:RHE_CH02951.
PATRICi23087517. VBIRhiEtl108884_3409.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC91718.1.
RefSeqiWP_011426194.1. NC_007761.1.

3D structure databases

ProteinModelPortaliQ2K618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347834.RHE_CH02951.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC91718; ABC91718; RHE_CH02951.
GeneIDi24299445.
KEGGiret:RHE_CH02951.
PATRICi23087517. VBIRhiEtl108884_3409.

Phylogenomic databases

eggNOGiENOG4105CPQ. Bacteria.
COG1063. LUCA.
HOGENOMiHOG000294686.
KOiK00060.
OMAiINPHRLE.

Enzyme and pathway databases

UniPathwayiUPA00046; UER00505.
BioCyciRETL347834:GJJ0-2964-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
HAMAPiMF_00627. Thr_dehydrog. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR004627. L-Threonine_3-DHase.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00692. tdh. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTDH_RHIEC
AccessioniPrimary (citable) accession number: Q2K618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.