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Protein

Oxygen-dependent coproporphyrinogen-III oxidase

Gene

hemF

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation

Catalytic activityi

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117SubstrateUniRule annotation1
Metal bindingi121Divalent metal cationUniRule annotation1
Active sitei131Proton donorUniRule annotation1
Metal bindingi131Divalent metal cationUniRule annotation1
Metal bindingi169Divalent metal cationUniRule annotation1
Metal bindingi199Divalent metal cationUniRule annotation1
Sitei199Important for dimerizationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandMetal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00322

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)
Short name:
CPOUniRule annotation
Short name:
Coprogen oxidaseUniRule annotation
Short name:
CoproporphyrinogenaseUniRule annotation
Gene namesi
Name:hemFUniRule annotation
Ordered Locus Names:RHE_CH03046
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000001936 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001198141 – 303Oxygen-dependent coproporphyrinogen-III oxidaseAdd BLAST303

Proteomic databases

PRIDEiQ2K5S3

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi347834.RHE_CH03046

Structurei

3D structure databases

ProteinModelPortaliQ2K5S3
SMRiQ2K5S3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 135Substrate bindingUniRule annotation3
Regioni268 – 303Important for dimerizationUniRule annotationAdd BLAST36
Regioni286 – 288Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DBS Bacteria
COG0408 LUCA
HOGENOMiHOG000262768
KOiK00228
OMAiMDLTPYY

Family and domain databases

Gene3Di3.40.1500.10, 1 hit
HAMAPiMF_00333 Coprogen_oxidas, 1 hit
InterProiView protein in InterPro
IPR001260 Coprogen_oxidase_aer
IPR036406 Coprogen_oxidase_aer_sf
IPR018375 Coprogen_oxidase_CS
PANTHERiPTHR10755 PTHR10755, 1 hit
PfamiView protein in Pfam
PF01218 Coprogen_oxidas, 1 hit
PIRSFiPIRSF000166 Coproporphyri_ox, 1 hit
PRINTSiPR00073 COPRGNOXDASE
SUPFAMiSSF102886 SSF102886, 1 hit
PROSITEiView protein in PROSITE
PS01021 COPROGEN_OXIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q2K5S3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERPELPIGL PEDIEEKKTA ARNWFEGLRD TICASFEALE DELQGPLSDQ
60 70 80 90 100
EPGRFVAKDW SREEGAGGGG RMSMMEGRVF EKVGVHTSTV HGEFSPDFSA
110 120 130 140 150
QIPGAKEDPR FWASGISLIA HPVNPNVPAV HMNTRMVVTS SRWFGGGADL
160 170 180 190 200
TPVLSRRRTQ EDEDSQLFHK AMEIACRNHA VADYDAYKAW CDDYFFLKHR
210 220 230 240 250
NEPRGIGGIF YDWLHSSEEA GGWNADFAFT RDVGRAFAMV YPKIVRSNFN
260 270 280 290 300
KLWTEADRDE QLIRRGRYVE FNLLYDRGTI FGLKTGGNVE SILSSLPPVV

RWP
Length:303
Mass (Da):34,265
Last modified:March 7, 2006 - v1
Checksum:iC90941194A65A2AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA Translation: ABC91813.1
RefSeqiWP_011426286.1, NC_007761.1

Genome annotation databases

EnsemblBacteriaiABC91813; ABC91813; RHE_CH03046
GeneIDi24301161
KEGGiret:RHE_CH03046

Similar proteinsi

Entry informationi

Entry nameiHEM6_RHIEC
AccessioniPrimary (citable) accession number: Q2K5S3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 7, 2006
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health