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Protein

Oxygen-dependent coproporphyrinogen-III oxidase

Gene

hemF

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation

Catalytic activityi

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171SubstrateUniRule annotation
Metal bindingi121 – 1211Divalent cationUniRule annotation
Active sitei131 – 1311Proton donorUniRule annotation
Metal bindingi131 – 1311Divalent cationUniRule annotation
Metal bindingi169 – 1691Divalent cationUniRule annotation
Metal bindingi199 – 1991Divalent cationUniRule annotation
Sitei199 – 1991Important for dimerizationUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciRETL347834:GJJ0-3060-MONOMER.
UniPathwayiUPA00251; UER00322.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)
Short name:
CPOUniRule annotation
Short name:
Coprogen oxidaseUniRule annotation
Short name:
CoproporphyrinogenaseUniRule annotation
Gene namesi
Name:hemFUniRule annotation
Ordered Locus Names:RHE_CH03046
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000001936 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Oxygen-dependent coproporphyrinogen-III oxidasePRO_1000119814Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi347834.RHE_CH03046.

Structurei

3D structure databases

ProteinModelPortaliQ2K5S3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1353Substrate bindingUniRule annotation
Regioni268 – 30336Important for dimerizationUniRule annotationAdd
BLAST
Regioni286 – 2883Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DBS. Bacteria.
COG0408. LUCA.
HOGENOMiHOG000262768.
KOiK00228.
OMAiMNTRMFW.
OrthoDBiEOG67T5GJ.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas.
InterProiIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2K5S3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERPELPIGL PEDIEEKKTA ARNWFEGLRD TICASFEALE DELQGPLSDQ
60 70 80 90 100
EPGRFVAKDW SREEGAGGGG RMSMMEGRVF EKVGVHTSTV HGEFSPDFSA
110 120 130 140 150
QIPGAKEDPR FWASGISLIA HPVNPNVPAV HMNTRMVVTS SRWFGGGADL
160 170 180 190 200
TPVLSRRRTQ EDEDSQLFHK AMEIACRNHA VADYDAYKAW CDDYFFLKHR
210 220 230 240 250
NEPRGIGGIF YDWLHSSEEA GGWNADFAFT RDVGRAFAMV YPKIVRSNFN
260 270 280 290 300
KLWTEADRDE QLIRRGRYVE FNLLYDRGTI FGLKTGGNVE SILSSLPPVV

RWP
Length:303
Mass (Da):34,265
Last modified:March 7, 2006 - v1
Checksum:iC90941194A65A2AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC91813.1.
RefSeqiWP_011426286.1. NC_007761.1.

Genome annotation databases

EnsemblBacteriaiABC91813; ABC91813; RHE_CH03046.
GeneIDi24301161.
KEGGiret:RHE_CH03046.
PATRICi23087721. VBIRhiEtl108884_3511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000133 Genomic DNA. Translation: ABC91813.1.
RefSeqiWP_011426286.1. NC_007761.1.

3D structure databases

ProteinModelPortaliQ2K5S3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347834.RHE_CH03046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC91813; ABC91813; RHE_CH03046.
GeneIDi24301161.
KEGGiret:RHE_CH03046.
PATRICi23087721. VBIRhiEtl108884_3511.

Phylogenomic databases

eggNOGiENOG4105DBS. Bacteria.
COG0408. LUCA.
HOGENOMiHOG000262768.
KOiK00228.
OMAiMNTRMFW.
OrthoDBiEOG67T5GJ.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00322.
BioCyciRETL347834:GJJ0-3060-MONOMER.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas.
InterProiIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFN 42 / ATCC 51251.

Entry informationi

Entry nameiHEM6_RHIEC
AccessioniPrimary (citable) accession number: Q2K5S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 7, 2006
Last modified: July 6, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.